Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,709,561 |
(C)6→7 |
coding (915/1692 nt) |
eptB ← |
Kdo2‑lipid A phosphoethanolamine 7''‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,709,555 | 1 | . | C | 100.0%
| 84.9
/ NA
| 25 | G307G (GGC→GGG) | eptB | Kdo2‑lipid A phosphoethanolamine 7''‑transferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (9/16); total (9/16) |
ATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAC > NC_000913/3709433‑3709685
|
aTCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa < 1:345142/140‑1 (MQ=255)
tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaaa > 2:165461/1‑140 (MQ=255)
gCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa < 2:193224/140‑1 (MQ=255)
acaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGagag < 1:216444/140‑1 (MQ=255)
aTTTCGCTCTGCATAGCGTAGAGGTCAGAGCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGtt > 2:210144/1‑140 (MQ=255)
cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:391965/140‑1 (MQ=255)
cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 2:409190/140‑1 (MQ=255)
ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg < 1:223069/140‑1 (MQ=255)
ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg < 2:445516/140‑1 (MQ=255)
tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 2:325609/140‑1 (MQ=255)
tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc > 1:418389/1‑140 (MQ=255)
tGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:227737/140‑1 (MQ=255)
aGCGTAGAGGTCAGAGCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 1:210144/140‑1 (MQ=255)
aGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 1:165461/140‑1 (MQ=255)
aGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 2:283191/140‑1 (MQ=255)
gCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac < 2:418389/140‑1 (MQ=255)
agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTTAGAGTTTGGTTGCGGTATCACAGGa > 2:509501/1‑140 (MQ=255)
agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt > 2:60796/1‑140 (MQ=255)
aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAg > 1:435385/1‑140 (MQ=255)
ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTg > 2:137217/1‑140 (MQ=255)
gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg < 1:169330/140‑1 (MQ=255)
ttttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTa > 2:463226/1‑140 (MQ=255)
gcgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa < 2:461120/140‑1 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCAt > 2:204450/1‑140 (MQ=255)
tCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc < 1:463226/140‑1 (MQ=255)
|
ATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAC > NC_000913/3709433‑3709685
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
