Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F30 I1 R1	19	54.8	1864302	98.6%	1838201	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,405,459	G→T	R258L (CGT→CTT)	purA →	adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,405,459	0	G	T	100.0%	139.2 / NA	40	R258L (CGT→CTT)	purA	adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (21/19); total (21/19)

ATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAC  >  NC_000913/4405336‑4405591

aTTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGtt                                                                                                                      <  1:659798/140‑1 (MQ=255)

            ttGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTc                                                                                                          <  1:75798/140‑1 (MQ=255)

               aaGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCaaa                                                                                                       >  1:729532/1‑140 (MQ=255)

                  gTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCt                                                                                                    <  2:691800/140‑1 (MQ=255)

                        aGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTcc                                                                                              <  2:473783/140‑1 (MQ=255)

                           gTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACt                                                                                           <  2:715536/140‑1 (MQ=255)

                                 tgctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCgtgt                                                                                      >  2:831588/1‑139 (MQ=255)

                                 tgctgGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCgtgt                                                                                      <  1:831588/139‑1 (MQ=255)

                                      gATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGc                                                                                <  1:705532/140‑1 (MQ=255)

                                               cACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGtt                                                                       <  2:729532/140‑1 (MQ=255)

                                                aCGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTg                                                                                 <  1:395621/129‑1 (MQ=255)

                                                aCGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTg                                                                                 >  2:395621/1‑129 (MQ=255)

                                                 cGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTcc                                                                     <  2:52017/140‑1 (MQ=255)

                                                   gTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCccg                                                                   >  1:60235/1‑140 (MQ=255)

                                                         aTCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGCGTAGGTGCAGGTCCGTTCCCGACCGaa                                                             >  2:365485/1‑140 (MQ=255)

                                                             gtacgtaACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGt                                                         <  2:303096/140‑1 (MQ=255)

                                                              tacgtaACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGtt                                                        >  2:694533/1‑140 (MQ=255)

                                                              tacgtaACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGtt                                                        >  1:121640/1‑140 (MQ=255)

                                                                   aaCTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTtgatg                                                   >  1:221324/1‑140 (MQ=255)

                                                                    aCTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTtgatga                                                  >  1:649752/1‑140 (MQ=255)

                                                                        cttcCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACt                                              >  1:408779/1‑140 (MQ=255)

                                                                          tcCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTgg                                            >  2:206696/1‑140 (MQ=255)

                                                                            cAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCg                                          <  1:694533/140‑1 (MQ=255)

                                                                              acacCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAg                                        <  2:649752/140‑1 (MQ=255)

                                                                                  cacTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTcc                                    >  2:692720/1‑140 (MQ=255)

                                                                                       ctgGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGc                               <  2:408779/140‑1 (MQ=255)

                                                                                          gtggcgtggcGACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAg                            >  1:336050/1‑140 (MQ=255)

                                                                                            ggcgtggcgACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCa                          <  1:353425/140‑1 (MQ=255)

                                                                                              cgtggcgACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAgg                        <  1:692720/140‑1 (MQ=255)

                                                                                               gtggcgACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAggg                       >  1:609442/1‑140 (MQ=255)

                                                                                                 ggcgACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTa                     <  2:221324/140‑1 (MQ=255)

                                                                                                 ggcgACCGGTTCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTa                     >  2:434336/1‑140 (MQ=255)

                                                                                                           tCCGGCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCgg           <  1:206696/140‑1 (MQ=255)

                                                                                                               gCCTGGGCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCa       >  1:918138/1‑140 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTTCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAAc     >  2:343736/1‑137 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTTCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAAc     <  1:343736/137‑1 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAc  >  2:532090/1‑140 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAc  >  2:481044/1‑140 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAc  >  2:404271/1‑140 (MQ=255)

                                                                                                                    ggCCCGCTTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAc  >  1:182225/1‑140 (MQ=255)

ATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATTACGTTCTGGGTATCCTCAAAGCTTACTCCACTCGTGTAGGTGCAGGTCCGTTCCCGACCGAACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTAC  >  NC_000913/4405336‑4405591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A