Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A26 F30 I1 R1	19	50.2	1707380	99.2%	1693720	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,893,898	C→A	G414G (GGC→GGA)	ygcN →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,893,898	0	C	A	100.0%	150.1 / NA	44	G414G (GGC→GGA)	ygcN	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/22); total (22/22)

GATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCT  >  NC_000913/2893762‑2894031

gATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    <  2:480022/140‑1 (MQ=255)

      aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    >  2:63710/1‑134 (MQ=255)

      aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGAt                                                                                                                                    <  1:63710/134‑1 (MQ=255)

          tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  1:849740/1‑140 (MQ=255)

          tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  1:110034/1‑140 (MQ=255)

          tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  2:816549/1‑140 (MQ=255)

          tACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGa                                                                                                                          >  2:549655/1‑140 (MQ=255)

               ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  2:278498/1‑140 (MQ=255)

               ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  2:302646/1‑140 (MQ=255)

               ggCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTg                                                                                                                     >  1:758068/1‑140 (MQ=255)

                gCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    <  1:575063/140‑1 (MQ=255)

                   tGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGAt                                                                                                                 <  1:816549/140‑1 (MQ=255)

                     cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGt                                                                                                               >  2:222170/1‑140 (MQ=255)

                     cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGt                                                                                                               >  1:650749/1‑140 (MQ=255)

                         aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGt                                                                                                           >  2:616846/1‑140 (MQ=255)

                           tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    >  2:806957/1‑129 (MQ=255)

                           tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGc                                                                                                                    <  1:806957/129‑1 (MQ=255)

                           tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAg                                                                                                         <  2:756715/140‑1 (MQ=255)

                               ggATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCg                                                                                                     >  1:312253/1‑140 (MQ=255)

                                  tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTaa                                                                                                  >  1:566300/1‑140 (MQ=255)

                                         cgcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTgg                                                                                           <  2:849740/140‑1 (MQ=255)

                                          gcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGgc                                                                                          >  2:473854/1‑140 (MQ=255)

                                           cgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGgcg                                                                                         <  2:432469/140‑1 (MQ=255)

                                                   gggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTg                                                                                 <  2:566300/140‑1 (MQ=255)

                                                   gggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTg                                                                                 <  2:110034/140‑1 (MQ=255)

                                                        cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATgcg                                                                            <  1:616846/140‑1 (MQ=255)

                                                        cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCATTGCTGATgcg                                                                            <  1:473854/140‑1 (MQ=255)

                                                               aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGcgcc                                                                          <  2:262074/135‑1 (MQ=255)

                                                               aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGcgcc                                                                          >  1:262074/1‑135 (MQ=255)

                                                               aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGcaca                                                                     >  2:758353/1‑140 (MQ=255)

                                                               aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGcaca                                                                     <  1:278498/140‑1 (MQ=255)

                                                                    ccTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGtt                                                                <  2:147556/140‑1 (MQ=255)

                                                                       gTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCg                                                             >  1:780244/1‑140 (MQ=255)

                                                                                           aTTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCa                                         <  2:312253/140‑1 (MQ=255)

                                                                                           aTTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCa                                         <  1:549655/140‑1 (MQ=255)

                                                                                            ttCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAg                                        <  1:758353/140‑1 (MQ=255)

                                                                                                      atTTACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAAc                              >  2:331076/1‑140 (MQ=255)

                                                                                                        ttACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAACGCCAGACGGCAGAACgg                            <  2:841726/140‑1 (MQ=255)

                                                                                                         tACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGt                           <  2:758068/140‑1 (MQ=255)

                                                                                                         tACGTCGTCACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGt                           <  1:302646/140‑1 (MQ=255)

                                                                                                                 cACGGCCTGTGGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAAc                   >  1:852006/1‑140 (MQ=255)

                                                                                                                          tgGCATCTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCg          >  1:125761/1‑140 (MQ=255)

                                                                                                                                cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGt    <  2:780244/140‑1 (MQ=255)

                                                                                                                                cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGt    >  1:387921/1‑140 (MQ=255)

                                                                                                                                cTGGCGGGAGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTCGATTAACGCCTGTCCGGCAggc    >  2:473427/1‑139 (MQ=255)

                                                                                                                                  ggcgggagATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGCCt  >  1:533108/1‑140 (MQ=255)

GATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCT  >  NC_000913/2893762‑2894031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A