Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F30 I1 R1	24	51.0	1727166	99.1%	1711621	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469,892	G→T	M254I (ATG→ATT)	yfdI →	CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469,892	0	G	T	100.0%	181.1 / NA	52	M254I (ATG→ATT)	yfdI	CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (29/23); total (29/23)

ATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATGCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCA  >  NC_000913/2469757‑2470027

aTCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCAt                                                                                                                                     >  1:576210/1‑140 (MQ=255)

   tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTcc                                                                                                                                       >  2:389982/1‑135 (MQ=255)

   tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGt                                                                                                                                  <  1:157447/140‑1 (MQ=255)

   tGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGt                                                                                                                                  <  1:528513/140‑1 (MQ=255)

      aaTATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATg                                                                                                                               <  2:373559/140‑1 (MQ=255)

      aaTATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATg                                                                                                                               <  2:838831/140‑1 (MQ=255)

          tGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtga                                                                                                                           >  2:190889/1‑140 (MQ=255)

          tGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtga                                                                                                                           <  1:559965/140‑1 (MQ=255)

           gCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          <  1:227866/140‑1 (MQ=255)

            cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  1:165987/1‑140 (MQ=255)

            cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  2:64852/1‑140 (MQ=255)

            cGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgatg                                                                                                                         >  1:526750/1‑140 (MQ=255)

                 tgtgCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          >  1:144250/1‑134 (MQ=255)

                 tgtgCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTtgat                                                                                                                          <  2:144250/134‑1 (MQ=255)

                    gcttgcttGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgt                                                                                                                 <  1:170455/140‑1 (MQ=255)

                      ttgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgtgt                                                                                                               <  2:291321/140‑1 (MQ=255)

                      ttgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAgtgtgt                                                                                                               <  2:171759/140‑1 (MQ=255)

                       tgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAg                                                                                                                    >  1:411031/1‑134 (MQ=255)

                       tgcttgcGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAg                                                                                                                    <  2:411031/134‑1 (MQ=255)

                             cGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTa                                                                                                        >  1:471554/1‑140 (MQ=255)

                                  atatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGc                                                                                                   >  2:791291/1‑140 (MQ=255)

                                   tatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGcc                                                                                                  <  1:466114/140‑1 (MQ=255)

                                   tatTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGcc                                                                                                  <  2:409461/140‑1 (MQ=255)

                                                 tATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAAtt                                                                                    <  1:190889/140‑1 (MQ=255)

                                                  aTTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTc                                                                                   >  1:361630/1‑140 (MQ=255)

                                                        aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCg                                                                                  <  2:434476/135‑1 (MQ=255)

                                                        aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCg                                                                                  >  1:434476/1‑135 (MQ=255)

                                                        aaGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACata                                                                             >  2:707683/1‑140 (MQ=255)

                                                        aaGGTGATGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACata                                                                             >  1:48836/1‑140 (MQ=255)

                                                              ttgttgAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCAttt                                                                       >  2:736781/1‑140 (MQ=255)

                                                              ttgttgAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCAttt                                                                       >  2:311069/1‑140 (MQ=255)

                                                                   gAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGgcgc                                                                  <  1:736781/140‑1 (MQ=255)

                                                                              gTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAAc                                                               <  2:801176/132‑1 (MQ=255)

                                                                              gTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAAc                                                               >  1:801176/1‑132 (MQ=255)

                                                                                   aTGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGtt                                                  >  2:634618/1‑140 (MQ=255)

                                                                                    tGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTg                                                 >  1:46592/1‑140 (MQ=255)

                                                                                    tGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTg                                                 >  1:784339/1‑140 (MQ=255)

                                                                                              ttCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATg                                       >  1:103987/1‑140 (MQ=255)

                                                                                                cGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTTGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGtt                                     >  1:540857/1‑140 (MQ=255)

                                                                                                    aGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGa                                 <  2:471554/140‑1 (MQ=255)

                                                                                                    aGTTATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGa                                 <  1:536126/140‑1 (MQ=255)

                                                                                                      ttATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAAt                               <  2:90891/140‑1 (MQ=255)

                                                                                                      ttATCTTTATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAAt                               <  2:70640/140‑1 (MQ=255)

                                                                                                            ttATATGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCaca                         <  1:528638/140‑3 (MQ=255)

                                                                                                                atGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGa                     >  2:9377/1‑140 (MQ=255)

                                                                                                                atGAAAAACAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGa                     >  2:135552/1‑140 (MQ=255)

                                                                                                                    aaaaCAACGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGaa                 >  1:397403/1‑140 (MQ=255)

                                                                                                                       acaacGGGTATAAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACg              >  1:500213/1‑140 (MQ=255)

                                                                                                                                ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  2:467027/1‑140 (MQ=255)

                                                                                                                                ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  1:793129/1‑140 (MQ=255)

                                                                                                                                ataAAATTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAAt     >  2:28983/1‑140 (MQ=255)

                                                                                                                                   aaaTTCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTTGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCa  <  2:540857/140‑1 (MQ=255)

ATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTGAATTAAATAAGTACCATGCAACATACTTCGGTAGTTATCTTTATATGAAAAACAACGGGTATAAAATGCCATCGTATGTTGATGATAAGTGTGTTGGGTTAGATGCCTGGGGTAATAAATTCGACATATCATTTGGCGCAACCCCAACAGAAGTTGGAACGGAATGTTTCGAATCTCATAAAGATGAAACGTTTTCGAATGCA  >  NC_000913/2469757‑2470027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A