Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F30 I1 R1	24	51.0	1727166	99.1%	1711621	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,236	C→A	E175D (GAG→GAT)	dapA ←	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,236	0	C	A	100.0%	150.0 / NA	43	E175D (GAG→GAT)	dapA	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/20); total (23/20)

TTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACA  >  NC_000913/2599104‑2599359

tttGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGa                                                                                                                      >  1:621737/1‑140 (MQ=255)

          tGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTac                                                                                                            <  2:621737/140‑1 (MQ=255)

            ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTacac                                                                                                          >  2:6525/1‑140 (MQ=255)

             gCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACAcg                                                                                                         <  2:610403/140‑1 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACAcg                                                                                                         <  2:671416/136‑1 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACAcg                                                                                                         >  1:671416/1‑136 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGtt                                                                                                     <  2:573317/140‑1 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGtt                                                                                                     >  2:220678/1‑140 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGtt                                                                                                     >  2:19450/1‑140 (MQ=255)

                 tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGtt                                                                                                     <  1:758270/140‑1 (MQ=255)

                        cgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTcc                                                                                              >  2:713902/1‑140 (MQ=255)

                           tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTg                                                                                           >  2:257912/1‑140 (MQ=255)

                             cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTGCCTGtt                                                                                         >  1:788043/1‑140 (MQ=255)

                             cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGtt                                                                                         <  2:622379/140‑1 (MQ=255)

                               aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGc                                                                                       <  1:6525/140‑1 (MQ=255)

                               aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGc                                                                                       <  1:713902/140‑1 (MQ=255)

                                        gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGAt                                                                              <  1:534581/140‑1 (MQ=255)

                                                              cGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTc                                                        >  2:485084/1‑140 (MQ=255)

                                                                cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                      >  1:409602/1‑140 (MQ=255)

                                                                cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                      >  2:458118/1‑140 (MQ=255)

                                                                cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                      >  2:345182/1‑140 (MQ=255)

                                                                cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                      >  2:124206/1‑140 (MQ=255)

                                                                cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                      >  1:540798/1‑140 (MQ=255)

                                                                         aTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGcc                                             <  1:485084/140‑1 (MQ=255)

                                                                             aGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAcc                                         <  1:220678/140‑1 (MQ=255)

                                                                               tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGcc                                             >  2:688879/1‑134 (MQ=255)

                                                                               tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGcc                                             <  1:688879/134‑1 (MQ=255)

                                                                               tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGt                                       >  1:852748/1‑140 (MQ=255)

                                                                                  aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGc                                              >  1:417911/1‑130 (MQ=255)

                                                                                  aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGc                                              <  2:417911/130‑1 (MQ=255)

                                                                                      cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                <  1:458118/140‑1 (MQ=255)

                                                                                      cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                >  2:570566/1‑140 (MQ=255)

                                                                                         tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGc                             <  1:124206/140‑1 (MQ=255)

                                                                                         tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGc                             >  1:608534/1‑140 (MQ=255)

                                                                                                   gccgcTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                   <  2:788043/140‑1 (MQ=255)

                                                                                                   gccgcTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                   >  1:125395/1‑140 (MQ=255)

                                                                                                   gccgcTCAGCAGAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                   <  2:540798/140‑1 (MQ=255)

                                                                                                            cagAACAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACggg          >  2:209706/1‑140 (MQ=255)

                                                                                                               aaCAAAATCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg       <  2:417472/140‑1 (MQ=255)

                                                                                                                    aaTCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca  <  2:125395/140‑1 (MQ=255)

                                                                                                                    aaTCATCTGAAACCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca  >  1:522527/1‑140 (MQ=255)

                                                                                                                               aCCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa         <  2:587170/122‑1 (MQ=255)

                                                                                                                               aCCAGATCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa         >  1:587170/1‑122 (MQ=255)

TTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACA  >  NC_000913/2599104‑2599359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A