Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F30 I1 R1	24	51.0	1727166	99.1%	1711621	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,588,041	G→T	G275G (GGC→GGA)	yjiA ←	P‑loop guanosine triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,588,041	0	G	T	100.0%	112.9 / NA	33	G275G (GGC→GGA)	yjiA	P‑loop guanosine triphosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/19); total (14/19)

CTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCA  >  NC_000913/4587908‑4588174

cTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTgg                                                                                                                                 >  2:353465/1‑140 (MQ=255)

      tCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAAcagc                                                                                                                           <  2:826795/140‑1 (MQ=255)

         tCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCCCCCCACGGCCTGTCCCAGTCGGCGCCGTAGAGGCGCTGGACCCCCCGGAACAGCAgg                                                                                                                        <  2:635817/140‑1 (MQ=255)

             ccccGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGt                                                                                                                    >  1:1548/1‑140 (MQ=255)

             ccccGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGt                                                                                                                    >  1:543894/1‑140 (MQ=255)

               ccGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTa                                                                                                                  >  2:197785/1‑140 (MQ=255)

                   aaaCGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGtt                                                                                                              <  1:436729/140‑1 (MQ=255)

                         aGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGt                                                                                                        <  1:218775/140‑1 (MQ=255)

                             cGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAAt                                                                                                    <  2:68706/140‑1 (MQ=255)

                              gAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATc                                                                                                   <  2:1548/140‑1 (MQ=255)

                              gAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATc                                                                                                   <  1:495132/140‑1 (MQ=255)

                                 tctcTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCcac                                                                                                >  1:238308/1‑140 (MQ=255)

                                   tctTCCTCCGGCAGCTGGGTCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAg                                                                                              <  2:238308/140‑1 (MQ=255)

                                   tctTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAg                                                                                              <  1:114207/140‑1 (MQ=255)

                                   tctTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAg                                                                                              <  1:643983/140‑1 (MQ=255)

                                   tctTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAg                                                                                              <  2:535024/140‑1 (MQ=255)

                                        ctccGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATcc                                                                                         <  1:536888/140‑1 (MQ=255)

                                        ctccGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATcc                                                                                         <  1:353465/140‑1 (MQ=255)

                                             gCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCCGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTg                                                                                    >  2:5789/1‑140 (MQ=255)

                                              cAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCCGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGt                                                                                   <  1:5789/140‑1 (MQ=255)

                                                           aTAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGttt                                                                      >  2:640889/1‑140 (MQ=255)

                                                                  ccATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATccgcc                                                               <  1:608656/140‑1 (MQ=255)

                                                                    aTCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGTATCCCTTTGGAACGGAGCAGTTTATCCGCCGa                                                             >  1:800456/1‑140 (MQ=255)

                                                                        tGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTcc                                                         >  1:218430/1‑140 (MQ=255)

                                                                                            ccccACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCcagcagc                                                   >  1:817555/1‑126 (MQ=255)

                                                                                            ccccACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCcagcagc                                                   <  2:817555/126‑1 (MQ=255)

                                                                                                    ccTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGaaa                             <  2:218430/140‑1 (MQ=255)

                                                                                                       gTCCCAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACtt                          >  2:463974/1‑140 (MQ=255)

                                                                                                         cccAGTCGGCGCTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                        <  1:640889/140‑1 (MQ=255)

                                                                                                                  cgcTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCt               >  2:538542/1‑140 (MQ=255)

                                                                                                                   gcTGTAGAGGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa              <  1:538542/140‑1 (MQ=255)

                                                                                                                         gagGCGCTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGt        <  1:463974/140‑1 (MQ=255)

                                                                                                                               cTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCa  >  2:128546/1‑140 (MQ=255)

                                                                                                                               cTGGACTCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCa  >  2:379384/1‑140 (MQ=255)

CTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCA  >  NC_000913/4587908‑4588174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A