Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A35 F30 I1 R1	29	21.9	747794	99.4%	743307	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,621	G→T	L95F (TTG→TTT)	yibA →	putative lyase containing HEAT‑repeat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,621	0	G	T	100.0%	85.9 / NA	26	L95F (TTG→TTT)	yibA	putative lyase containing HEAT‑repeat
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/12); total (14/12)

GAGGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTG  >  NC_000913/3766485‑3766751

gagGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAAc                                                                                                                                 >  1:207479/1‑140 (MQ=255)

     gggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAatat                                                                                                                            >  2:329133/1‑140 (MQ=255)

      ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAat                                                                                                                              <  2:96832/137‑1 (MQ=255)

      ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAat                                                                                                                              >  1:96832/1‑137 (MQ=255)

      ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATg                                                                                                                           >  1:40537/1‑140 (MQ=255)

         aaGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCa                                                                                                                        <  2:75728/140‑1 (MQ=255)

          aGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCAt                                                                                                                       <  2:165022/140‑1 (MQ=255)

           gACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCAtt                                                                                                                      >  2:55526/1‑140 (MQ=255)

            aCGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTg                                                                                                                     <  2:306219/140‑1 (MQ=255)

                            aaTTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCtt                                                                                                     >  2:322409/1‑140 (MQ=255)

                                 aGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGtt                                                                                                <  2:69665/140‑1 (MQ=255)

                                     cTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAg                                                                                            >  1:104243/1‑140 (MQ=255)

                                             aTAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCa                                                                                    <  1:92988/140‑1 (MQ=37)

                                                aaaaTTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATc                                                                                 <  2:6472/140‑1 (MQ=25)

                                                    ttATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGt                                                                             >  2:205355/1‑140 (MQ=25)

                                                       tatCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCaa                                                                          >  1:299883/1‑140 (MQ=25)

                                                       tatCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCaa                                                                          <  2:104243/140‑1 (MQ=25)

                                                                    atCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATg                                                             >  2:239537/1‑140 (MQ=255)

                                                                                   cTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                              <  1:337490/140‑1 (MQ=255)

                                                                                     cGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAttt                                            >  2:187825/1‑140 (MQ=255)

                                                                                      gggCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                           >  1:308104/1‑140 (MQ=255)

                                                                                        gCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTAtt                                         >  2:4216/1‑140 (MQ=255)

                                                                                              aaaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTa                                   <  2:49391/140‑1 (MQ=255)

                                                                                               aaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                                  <  2:255768/140‑1 (MQ=255)

                                                                                               aaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                                  <  2:135673/140‑1 (MQ=255)

                                                                                                                               tAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTg  >  2:264842/1‑140 (MQ=255)

GAGGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTG  >  NC_000913/3766485‑3766751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A