Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A35 F30 I1 R1 29 21.9 747794 99.4% 743307 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,194,562 C→A G513V (GGG→GTG)  thiC ← phosphomethylpyrimidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,194,5620CA100.0% 51.1 / NA 17G513V (GGG→GTG) thiCphosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/9);  total (8/9)

TCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAATA  >  NC_000913/4194427‑4194696
                                                                                                                                       |                                                                                                                                      
tCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTGCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGcacccg                                                                                                                                    >  2:257558/1‑138 (MQ=255)
                       ttCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCa                                                                                                             <  1:45600/140‑1 (MQ=255)
                                   ttCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGct                                                                                                 >  2:143935/1‑140 (MQ=255)
                                          cGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTgg                                                                                          <  2:157906/140‑1 (MQ=255)
                                                     cATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAAc                                                                               <  1:6614/140‑1 (MQ=255)
                                                                                 ggATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAat                                                   <  1:143935/140‑1 (MQ=255)
                                                                                           tCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCa                                         <  1:66734/140‑1 (MQ=255)
                                                                                                            tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCAc                                        <  2:31128/124‑1 (MQ=255)
                                                                                                            tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCAc                                        >  1:31128/1‑124 (MQ=255)
                                                                                                            tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGaa                        <  1:233068/140‑1 (MQ=255)
                                                                                                                                gATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCaa    >  2:7439/1‑140 (MQ=255)
                                                                                                                                gATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCaa    <  2:136541/140‑1 (MQ=255)
                                                                                                                                 aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCgg         <  2:257373/134‑1 (MQ=255)
                                                                                                                                 aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCgg         >  1:257373/1‑134 (MQ=255)
                                                                                                                                 aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  1:295243/1‑140 (MQ=255)
                                                                                                                                 aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  2:279320/1‑140 (MQ=255)
                                                                                                                                 aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  1:120838/1‑140 (MQ=255)
                                                                                                                                  tAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAata  >  1:128332/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAATA  >  NC_000913/4194427‑4194696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A