Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A35 F30 I1 R1
|
29 |
21.9 |
747794 |
99.4% |
743307 |
139.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,374,924 |
C→A |
R245L (CGG→CTG) |
epmB ← |
lysine 2,3‑aminomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,374,924 | 0 | C | A | 100.0%
| 85.2
/ NA
| 25 | R245L (CGG→CTG) | epmB | lysine 2,3‑aminomutase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/11); total (14/11) |
TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGA > NC_000913/4374793‑4375056
|
tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAAc > 1:86788/1‑140 (MQ=255)
aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCg > 2:341098/1‑140 (MQ=255)
tACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGaa > 2:83684/1‑140 (MQ=255)
cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATg < 1:269081/140‑1 (MQ=255)
tACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAt < 1:306176/140‑1 (MQ=255)
ccGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAc < 2:185966/140‑1 (MQ=255)
cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCa < 1:239322/140‑1 (MQ=255)
aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCAt > 1:228404/1‑140 (MQ=255)
acaacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCa < 1:58285/140‑1 (MQ=255)
aGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGAtt > 2:77606/1‑140 (MQ=255)
tGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCa > 1:289896/1‑140 (MQ=255)
aTCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAg < 2:357134/140‑1 (MQ=255)
tCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGa > 2:259464/1‑140 (MQ=255)
gTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGCAGAACGGGCAAAGCAt > 1:95693/1‑140 (MQ=255)
aaCGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCa > 1:43371/1‑140 (MQ=255)
aCGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAg < 1:89688/140‑1 (MQ=255)
aCGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAg < 2:228404/140‑1 (MQ=255)
aCGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAg > 1:70675/1‑140 (MQ=255)
aCGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAg > 1:333183/1‑140 (MQ=255)
cGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAgc > 2:295767/1‑140 (MQ=255)
tctGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGcc > 2:278374/1‑140 (MQ=255)
aGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGAt < 2:108828/140‑1 (MQ=255)
gTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGc < 2:96482/140‑1 (MQ=255)
cGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCggg > 1:121755/1‑140 (MQ=255)
gCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGa < 1:341098/140‑1 (MQ=255)
|
TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGGCATGGTTGATGTGATTCACCAGCAAGATTTGCAGCGTAGAACGGGCAAAGCATTCAACCAGCGCCTCGGTGATACGTGCCGGGA > NC_000913/4374793‑4375056
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
