Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F30 I1 R1 25 26.9 915936 99.4% 910440 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,545,816 C→A S15* (TCG→TAG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,545,8160CA100.0% 92.5 / NA 28S15* (TCG→TAG) murQN‑acetylmuramic acid 6‑phosphate etherase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/16);  total (12/16)

AAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCA  >  NC_000913/2545700‑2545945
                                                                                                                    |                                                                                                                                 
aaGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGa                                                                                                            <  2:404847/140‑1 (MQ=255)
    cTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCt                                                                                                        >  1:298557/1‑140 (MQ=255)
        cAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGaa                                                                                                    <  2:143464/140‑1 (MQ=255)
              gggATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGc                                                                                              <  1:52831/140‑1 (MQ=255)
               ggATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGcc                                                                                             <  1:407609/140‑1 (MQ=255)
                        aTTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCa                                                                                    >  2:156861/1‑140 (MQ=255)
                        aTTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCa                                                                                    <  2:390401/140‑1 (MQ=255)
                            cATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGa                                                                                >  1:52883/1‑140 (MQ=255)
                             aTACATCTGCAATTATTTGATCGTAAATAGTCAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTTGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                                                               >  2:337095/1‑140 (MQ=255)
                             aTACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                                                               <  2:266476/140‑1 (MQ=255)
                             aTACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                                                               <  1:427548/140‑1 (MQ=255)
                             aTACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                                                               <  1:72196/140‑1 (MQ=255)
                              tACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATg                                                                              >  1:352842/1‑140 (MQ=255)
                                   cTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGAt                                                                         >  2:245516/1‑140 (MQ=255)
                                            tttGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTa                                                                <  2:52883/140‑1 (MQ=255)
                                                           tAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAg                                                 >  2:139026/1‑140 (MQ=255)
                                                           tAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAg                                                 >  1:143234/1‑140 (MQ=255)
                                                               gTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg                                             <  2:42848/140‑1 (MQ=255)
                                                               gTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg                                             <  1:170003/140‑1 (MQ=255)
                                                               gTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg                                             <  2:143234/140‑1 (MQ=255)
                                                                   ccaccGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACt                                         >  1:333787/1‑140 (MQ=255)
                                                                         aTGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGa                                   >  2:297697/1‑140 (MQ=255)
                                                                                   aaaaGATGATTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCcgcg                         <  1:245516/140‑1 (MQ=255)
                                                                                        atgatTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgat                    <  2:418032/140‑1 (MQ=255)
                                                                                        atgatTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgat                    <  2:9571/140‑1 (MQ=255)
                                                                                           atTACTGAAGGCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgatcga                 >  2:54531/1‑140 (MQ=255)
                                                                                                    ggCTCGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCa        >  1:262640/1‑140 (MQ=255)
                                                                                                        cGAACACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGcc    <  1:312144/140‑1 (MQ=255)
                                                                                                          aaCACCGCCTAGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCa  >  1:265700/1‑140 (MQ=255)
                                                                                                                    |                                                                                                                                 
AAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCA  >  NC_000913/2545700‑2545945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A