Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A30 F30 I1 R1	25	26.9	915936	99.4%	910440	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,523	G→T	G185V (GGC→GTC)	ilvC →	ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,523	0	G	T	100.0%	98.3 / NA	30	G185V (GGC→GTC)	ilvC	ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/13); total (17/13)

CGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTG  >  NC_000913/3958389‑3958646

cGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAg                                                                                                                        >  1:163595/1‑140 (MQ=255)

        tCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCg                                                                                                                <  2:182119/140‑1 (MQ=255)

         ccGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGa                                                                                                               <  2:444438/140‑1 (MQ=255)

                 atatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCaaa                                                                                                       >  2:58709/1‑140 (MQ=255)

                 atatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCaaa                                                                                                       >  2:338370/1‑140 (MQ=255)

                  tatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAg                                                                                                      >  1:379431/1‑140 (MQ=255)

                   atCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGc                                                                                                     >  1:215101/1‑140 (MQ=255)

                               aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         >  1:26531/1‑140 (MQ=255)

                               aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         >  1:292722/1‑140 (MQ=255)

                               aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         <  1:338370/140‑1 (MQ=255)

                                tGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCa                                                                                        >  2:326387/1‑140 (MQ=255)

                                      cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                  >  2:361195/1‑140 (MQ=255)

                                         cGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggtggt                                                                               <  2:118536/140‑1 (MQ=255)

                                                   aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  1:361195/140‑1 (MQ=255)

                                                   aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  2:36945/140‑1 (MQ=255)

                                                   aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  1:208533/140‑1 (MQ=255)

                                                           aaGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGgtg                                                                  <  1:285686/135‑1 (MQ=255)

                                                           aaGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGgtg                                                                  >  2:285686/1‑135 (MQ=255)

                                                                 gTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCg                                                       >  2:337024/1‑140 (MQ=255)

                                                                                   ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTg                                     >  2:56027/1‑140 (MQ=255)

                                                                                        ggCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATc                                >  2:188313/1‑140 (MQ=255)

                                                                                                  cGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCAACGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATg                      <  2:379431/140‑1 (MQ=255)

                                                                                                       aTTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                 <  2:292722/140‑1 (MQ=255)

                                                                                                        ttGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAg                <  1:170404/140‑1 (MQ=255)

                                                                                                             gTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATgg                     <  1:40038/130‑1 (MQ=255)

                                                                                                             gTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATgg                     >  2:40038/1‑130 (MQ=255)

                                                                                                              ttCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAAcc          <  1:56027/140‑1 (MQ=255)

                                                                                                              ttCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAAcc          >  1:396409/1‑140 (MQ=255)

                                                                                                                 aCCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATc       <  2:213043/140‑1 (MQ=255)

                                                                                                                   ccGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCt     >  1:324437/1‑140 (MQ=255)

                                                                                                                      gAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg  >  2:160937/1‑140 (MQ=255)

                                                                                                                      gAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg  >  1:294030/1‑140 (MQ=255)

CGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTG  >  NC_000913/3958389‑3958646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A