Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F30 I1 R1	18	47.5	1605326	99.2%	1592483	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,343,256	C→A	G305V (GGA→GTA)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,343,256	0	C	A	100.0%	113.2 / NA	34	G305V (GGA→GTA)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/8); total (26/8)

ATCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGC  >  NC_000913/2343124‑2343388

aTCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGc                                                                                                                               <  2:621447/140‑1 (MQ=255)

      ccAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATc                                                                                                                         <  2:313988/140‑1 (MQ=255)

                 aTTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACtt                                                                                                              <  2:197585/140‑1 (MQ=255)

                       aTCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTa                                                                                                        >  2:560871/1‑140 (MQ=255)

                       aTCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTa                                                                                                        >  1:759701/1‑140 (MQ=255)

                               tAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCgaga                                                                                                >  2:614198/1‑140 (MQ=255)

                               tAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCgaga                                                                                                <  1:678094/140‑1 (MQ=255)

                                      cAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACtt                                                                                                         <  2:285161/124‑1 (MQ=255)

                                      cAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACtt                                                                                                         >  1:285161/1‑124 (MQ=255)

                                         aTCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGc                                                                                      >  1:757291/1‑140 (MQ=255)

                                           cGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:614456/1‑140 (MQ=255)

                                           cGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:648004/1‑140 (MQ=255)

                                           cGCCGGAACCTGTTTTGGGGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa                                                                                    >  1:269500/1‑140 (MQ=255)

                                             ccGGACCTTTTTTTGGTGATTACCCCGGTACCAGAAATAGAGTCAACAGCCCCGTCATTC‑TCTGTATTGCCAAAAACCAGCGTTTTTCCGTCGGAAATACCAAAGGTAACTTCACTAAAGCCGAGATACAAAAAcccccc                                                                                  <  2:680915/140‑1 (MQ=255)

                                             ccGGAACCTGTTTTGGTGATTACCCCGGTACCAGAAAAAGAGTCAACAGCTCCTTCATTC‑TCTGTATTGCAAATAACCAGCTTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCAcc                                                                                  <  2:759701/140‑1 (MQ=255)

                                                         ttGGTGATTACCCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATACCCAGCGTTTTTACGTCGGCAATATCAAAGGTACCTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGagga                                                                      <  2:472567/140‑1 (MQ=255)

                                                           ggTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaag                                                                    >  1:154538/1‑140 (MQ=255)

                                                            gTGATTACCCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATACCCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaagg                                                                   <  1:529478/140‑1 (MQ=255)

                                                                 tAACCCGGTACCAGAAATAGAGTCAACACCTCCGTCTTTC‑TTTTTATTGCCAATACCCAGCTTTTTTCCGCCGGCAATATCAAAGGTACCTTCACTTAAGCCGAGATACAAAAACCCCCCCGCCGCAGAGGAAGGACCAt                                                              <  2:235650/140‑1 (MQ=255)

                                                                         tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:362304/1‑140 (MQ=255)

                                                                         tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:172212/1‑140 (MQ=255)

                                                                         tACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAACATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAg                                                      >  1:622446/1‑140 (MQ=255)

                                                                             aGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCctg                                                  >  1:481169/1‑140 (MQ=255)

                                                                                aaTAGAGTCAACAGCTCCGTCATTC‑TCTGTTTTGCAAATAACCAGCTTTTTTACGTCGGAAATATCAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgctg                                               <  2:479370/140‑1 (MQ=255)

                                                                                aaTAGAGACAACGGCT‑CGTTATTCTTCTGTATTCCCAATAACCAGCTTTTTCACGTCGCAAAGATAAAAGTTATCTTCTTTTAAGCCGAGATATAGAACCCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCctgctg                                               <  2:471545/140‑1 (MQ=255)

                                                                                  tAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTgcg                                             >  1:479370/1‑140 (MQ=255)

                                                                                  tAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTgcg                                             >  1:338140/1‑140 (MQ=255)

                                                                                     agTCACCAGCTCCGTCATTC‑TTTTTTTTAACAAAAACCAGCGTTTTCACGTCGACAATAAAAAAGGTACCTTCACTTACCCCGAGAAACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTa                                          <  2:690244/140‑1 (MQ=255)

                                                                                       tCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTAtt                                        >  2:777282/1‑140 (MQ=255)

                                                                                         aaCAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGt                                      >  1:681654/1‑140 (MQ=255)

                                                                                            aGCTCCTTCATTC‑TCTGTATTGCCAATAACCAGTTTTTTTACGTCGGCAATATAAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTtct                                   <  2:113017/140‑1 (MQ=255)

                                                                                            aGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTtct                                   >  1:85218/1‑140 (MQ=255)

                                                                                              cTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCa                                 >  1:113017/1‑140 (MQ=255)

                                                                                                 cGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCg                              >  2:280442/1‑140 (MQ=255)

                                                                                                 cGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCg                              >  1:330970/1‑140 (MQ=255)

                                                                                                   tctttC‑TCTGTATTGCAAATACCCAGCTTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTAAAGCCGAGATACATAAACCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGAc                            <  2:648004/137‑1 (MQ=255)

                                                                                                         gcttttATTCCAAATAACCTGCTTTTTTACGCCGAAAAAATAAAAGATACCTCGAGTTAAGCATAAAACTAAAAACCCAGCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAaca                       <  2:79782/135‑1 (MQ=255)

                                                                                                            tGTATTGCCAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGc                    >  2:216945/1‑140 (MQ=255)

                                                                                                                   ccAATAACCAGCTTTTTTACGTCGGCAATATCAAGGGTAACTTCTCTAAAGCCGAGATACAAAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGtt             <  1:560871/140‑1 (MQ=255)

                                                                                                                   ccAATAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGtt             >  1:410884/1‑140 (MQ=255)

                                                                                                                   caaaTACCCAGCGTTTTTACGTCGGCAATATAAAAGGTACCTTCACTAAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGACTCCGtt             <  2:481169/138‑1 (MQ=255)

                                                                                                                      aTAACCAGCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTcct          >  1:79782/1‑138 (MQ=255)

                                                                                                                          ccAGCTTTTTTACGTCGGAAATAAAAAAGGTAACTTCCCTTAAGCCGAGATAAATAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTg      <  2:338140/140‑1 (MQ=255)

                                                                                                                             gCGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAg   >  2:48723/1‑140 (MQ=255)

                                                                                                                              cGTTTTTACGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGc  >  1:144868/1‑140 (MQ=255)

ATCTCACCAGTAAAGTCATTGTTATCTGCATTAAGTACCAGATCGCCGGAACCTGTTTTGGTGATTAACCCGGTACCAGCAATAGAGTCAACAGCTCCGTCATTC‑TCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTCCGTTCTGGCTGTAGC  >  NC_000913/2343124‑2343388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A