Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F30 I1 R1
|
21 |
20.2 |
686622 |
99.1% |
680442 |
139.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,359,040 |
C→A |
L371L (CTG→CTT) |
yfaV ← |
putative transporter YfaV |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,359,040 | 0 | C | A | 100.0%
| 59.3
/ NA
| 18 | L371L (CTG→CTT) | yfaV | putative transporter YfaV |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/9); total (9/9) |
ACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTA > NC_000913/2358905‑2359167
|
acGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGt > 1:96536/1‑140 (MQ=255)
cAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCg > 2:137575/1‑140 (MQ=255)
gCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGca > 2:107282/1‑140 (MQ=255)
aacccGATGCCCGCCACCGCTCCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGcagc < 2:234887/137‑1 (MQ=255)
aCCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTccc > 2:300740/1‑133 (MQ=255)
aCCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTccc < 1:300740/133‑1 (MQ=255)
aTGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAgcgc < 1:326900/140‑1 (MQ=255)
gACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCg < 1:137575/140‑1 (MQ=255)
ccGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGaa < 2:188399/140‑1 (MQ=255)
cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCt > 1:317781/1‑140 (MQ=255)
cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCaa > 1:219900/1‑140 (MQ=255)
aTCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca < 2:96536/140‑1 (MQ=255)
aTCGGGGCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca < 2:219900/140‑1 (MQ=255)
ggCAATAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg > 1:247902/1‑140 (MQ=255)
tAAACCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg > 1:175890/1‑140 (MQ=255)
aCCCGCCCACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc < 2:175890/140‑1 (MQ=255)
ccACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAtt > 1:9038/1‑140 (MQ=255)
cACTGCACCAAAAAGGTTTACAAAACCAATTCCCGCAGCCAGCGCAGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTa < 2:6525/140‑1 (MQ=255)
|
ACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTA > NC_000913/2358905‑2359167
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
