Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F30 I1 R1	21	20.2	686622	99.1%	680442	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,477,797	G→T	S172* (TCG→TAG)	argI ←	ornithine carbamoyltransferase ArgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,477,797	0	G	T	100.0%	91.4 / NA	27	S172* (TCG→TAG)	argI	ornithine carbamoyltransferase ArgI
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/16); total (11/16)

AGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGC  >  NC_000913/4477664‑4477929

agtcagCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGc                                                                                                                                <  1:60175/140‑1 (MQ=255)

  tcagCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGccc                                                                                                                              <  2:80580/140‑1 (MQ=255)

                     aTTTTGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCAc                                                                                                           <  2:113705/140‑1 (MQ=255)

                         tGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGc                                                                                                       >  2:217128/1‑140 (MQ=255)

                         tGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTCGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGc                                                                                                       >  2:328499/1‑140 (MQ=255)

                              tgCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCTGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGa                                                                                                  >  1:251859/1‑140 (MQ=255)

                              tgCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGa                                                                                                  >  2:251559/1‑140 (MQ=255)

                                ccAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGAcc                                                                                                >  2:269251/1‑140 (MQ=255)

                                               ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg                                                                                 <  2:64321/140‑1 (MQ=255)

                                                         cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCGCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                                       <  1:169631/140‑1 (MQ=255)

                                                         cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                                       >  1:104521/1‑140 (MQ=255)

                                                         cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                                       >  1:237270/1‑140 (MQ=255)

                                                         cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc                                                                       >  2:292161/1‑140 (MQ=255)

                                                               ttCCGGCCAGCACGCTCGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa                                                                 <  1:328499/140‑1 (MQ=255)

                                                                        gCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCt                                                        >  1:75744/1‑140 (MQ=255)

                                                                         cACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg                                                       >  1:34991/1‑140 (MQ=255)

                                                                                        aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                                     >  2:25368/1‑127 (MQ=255)

                                                                                        aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa                                                     <  1:25368/127‑1 (MQ=255)

                                                                                                cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcagca                                <  2:242384/140‑1 (MQ=255)

                                                                                                   aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCt                             <  2:37994/140‑1 (MQ=255)

                                                                                                      cAGACCGGTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg                          <  1:34410/140‑1 (MQ=255)

                                                                                                             gTAAGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg                   <  1:292161/140‑1 (MQ=255)

                                                                                                               aaGCGCCGCAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGa                 <  1:217128/140‑1 (MQ=255)

                                                                                                                    ccgcAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa            <  1:92179/140‑1 (MQ=255)

                                                                                                                    ccgcAGCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa            <  1:184377/140‑1 (MQ=255)

                                                                                                                         gCTTCGAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGt       <  1:251559/140‑1 (MQ=255)

                                                                                                                              gAGCATCTAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc  <  1:269251/140‑1 (MQ=255)

AGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGC  >  NC_000913/4477664‑4477929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A