Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F30 I1 R1	26	45.9	1554102	99.2%	1541669	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,971,608	C→A	H4Q (CAC→CAA)	tas →	putative NADP(H)‑dependent aldo‑keto reductase Tas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,971,608	0	C	A	100.0%	108.5 / NA	32	H4Q (CAC→CAA)	tas	putative NADP(H)‑dependent aldo‑keto reductase Tas
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/19); total (13/19)

CAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCA  >  NC_000913/2971472‑2971721

cAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGt                                                                                                                <  2:43820/140‑1 (MQ=255)

 aaaTTATTGAACGTTAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGta                                                                                                               <  2:543031/140‑1 (MQ=255)

               tAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCg                                                                                                 >  1:52845/1‑140 (MQ=255)

               tAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCg                                                                                                 >  1:762324/1‑140 (MQ=255)

                    aaTAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGa                                                                                            >  2:284450/1‑140 (MQ=255)

                         ggTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGtt                                                                                                   <  1:275399/128‑1 (MQ=255)

                         ggTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGtt                                                                                                   >  2:275399/1‑128 (MQ=255)

                               cATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCa                                                                                       >  2:518756/1‑134 (MQ=255)

                               cATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCa                                                                                       <  1:518756/134‑1 (MQ=255)

                               cATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGc                                                                                 >  1:139370/1‑140 (MQ=255)

                                    ggCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTgggg                                                                            >  1:700865/1‑140 (MQ=255)

                                      cTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCt                                                                          <  2:700865/140‑1 (MQ=255)

                                          ccTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGc                                                                      <  1:376750/140‑1 (MQ=255)

                                          ccTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGc                                                                      >  1:775414/1‑140 (MQ=255)

                                               aGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGAt                                                                 <  1:158048/140‑1 (MQ=255)

                                                   ttttCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACg                                                             >  2:641086/1‑140 (MQ=255)

                                                            tcccttttcccttCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAGGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTgaa                                                    <  1:745418/140‑1 (MQ=255)

                                                                  ttcccttcccTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTgaacagaac                                              >  2:537670/1‑140 (MQ=255)

                                                                             ctGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGa                                   <  1:184347/140‑1 (MQ=255)

                                                                                     tttATATTCGTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACg                           <  1:677404/140‑1 (MQ=255)

                                                                                     tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACg                           <  2:139053/140‑1 (MQ=255)

                                                                                     tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACg                           <  2:701969/140‑1 (MQ=255)

                                                                                     tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACg                           <  2:374135/140‑1 (MQ=255)

                                                                                     tttATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGACACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACg                           <  2:166824/140‑1 (MQ=255)

                                                                                          atTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACaa                      <  2:610497/140‑1 (MQ=255)

                                                                                                tATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGAc                <  1:641086/140‑1 (MQ=255)

                                                                                                      gTGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGcc          >  2:13364/1‑140 (MQ=255)

                                                                                                       tGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCg         >  1:82813/1‑140 (MQ=255)

                                                                                                       tGATTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCg         <  2:762324/140‑1 (MQ=255)

                                                                                                         aTTATAAAAAGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGGTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTc       >  2:351056/1‑140 (MQ=255)

                                                                                                              aaaaaGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCa  <  2:775414/140‑1 (MQ=255)

                                                                                                              aaaaaGGAAACGGCTATGCAATATCAACGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCa  <  2:82813/140‑1 (MQ=255)

CAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGCTGGCCTTAAGATTTTTCTCTTTCCCTTTTCCCTTCCCTCTGCCATTTTTATATTCCTTATGTCGTGATTATAAAAAGGAAACGGCTATGCAATATCACCGTATACCCCACAGTTCGCTGGAAGTCAGCACGCTGGGGCTTGGCACGATGACGTTTGGTGAACAGAACAGCGAAGCCGACGCCCACGCACAACTCGACTATGCCGTCGCTCA  >  NC_000913/2971472‑2971721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A