Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F30 I1 R1	26	45.9	1554102	99.2%	1541669	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,387	G→T	R134L (CGA→CTA)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,387	0	G	T	100.0%	147.1 / NA	42	R134L (CGA→CTA)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/17); total (25/17)

TGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGT  >  NC_000913/3063250‑3063520

tGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCCGGCGCGTGCCGAAGCGGCGGTTATTCGCCCGGTACCATCCTCCGGTCATCTTGGCGGTGCCAGTCagctag                                                                                                                                     >  1:552938/1‑140 (MQ=255)

          cccAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAAtt                                                                                                                           >  1:686997/1‑140 (MQ=255)

              gcagcCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATg                                                                                                                       <  2:543229/140‑1 (MQ=255)

                  cccGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGAGAATTAATGCTGt                                                                                                                   >  2:74798/1‑140 (MQ=255)

                     ggTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTAt                                                                                                                >  2:756693/1‑140 (MQ=255)

                       tCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGc                                                                                                              >  2:38866/1‑140 (MQ=255)

                            ggCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGGGCCAGTCAGCTAGCGCGGGGATTAATGCTGTTATGCGAagc                                                                                                         >  2:492775/1‑140 (MQ=255)

                              cGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagcag                                                                                                       <  1:38866/140‑1 (MQ=255)

                              cGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagcag                                                                                                       <  1:756693/140‑1 (MQ=255)

                                 tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagca                                                                                                        >  2:651343/1‑136 (MQ=255)

                                 tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagca                                                                                                        <  1:651343/136‑1 (MQ=255)

                                 tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGGTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCgg                                                                                                    >  2:390341/1‑140 (MQ=255)

                                     aTTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGTGAAGCAGCGGGTTa                                                                                                <  2:686997/140‑1 (MQ=255)

                                     aTTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGAGAATTAATGCTGTTATGCGAAGCAGCGGGTTa                                                                                                <  1:74798/140‑1 (MQ=255)

                                                        gCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaa                                                                             <  1:655123/140‑1 (MQ=255)

                                                         cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:655484/1‑140 (MQ=255)

                                                         cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:694377/1‑140 (MQ=255)

                                                         cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:541372/1‑140 (MQ=255)

                                                         cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:476838/1‑140 (MQ=255)

                                                             aatgaCCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGt                                                                        <  1:177077/140‑1 (MQ=255)

                                                                      gcgcGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCgg                                                               >  2:613156/1‑140 (MQ=255)

                                                                       cgcgTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCggg                                                              <  1:293919/140‑1 (MQ=255)

                                                                           tGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGt                                                          >  1:687183/1‑140 (MQ=255)

                                                                                 aGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGaaa                                                    >  1:96407/1‑140 (MQ=255)

                                                                                 aGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGaaa                                                    <  2:21773/140‑1 (MQ=255)

                                                                                      cgTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGaa                                               <  1:613156/140‑1 (MQ=255)

                                                                                             tCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGccc                                        <  2:96407/140‑1 (MQ=255)

                                                                                             tCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGccc                                        <  2:655484/140‑1 (MQ=255)

                                                                                                  cGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtg                                   <  1:411043/140‑1 (MQ=255)

                                                                                                     tACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtggtg                                >  2:154385/1‑140 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGa                              >  2:397305/1‑132 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGa                              <  1:397305/132‑1 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  1:195741/1‑140 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:465283/1‑140 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:723910/1‑140 (MQ=255)

                                                                                                               cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:269347/1‑140 (MQ=255)

                                                                                                                  tCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTAtct                   >  2:698099/1‑140 (MQ=255)

                                                                                                                    aTCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCg                 >  2:662580/1‑140 (MQ=255)

                                                                                                                     tCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                >  1:755059/1‑140 (MQ=255)

                                                                                                                      cTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGtt               >  1:520633/1‑140 (MQ=255)

                                                                                                                         ggCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCa            >  2:451239/1‑140 (MQ=255)

                                                                                                                              tGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                >  2:132658/1‑131 (MQ=255)

                                                                                                                              tGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                <  1:132658/131‑1 (MQ=255)

                                                                                                                                ccAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGcc     >  1:544838/1‑140 (MQ=255)

                                                                                                                                ccAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGcc     >  2:712409/1‑140 (MQ=255)

                                                                                                                                   gTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGt  <  2:614072/140‑1 (MQ=255)

TGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGT  >  NC_000913/3063250‑3063520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A