Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F30 I1 R1	26	45.9	1554102	99.2%	1541669	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,192,822	G→A	W205* (TGG→TGA)	yqiJ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,192,822	0	G	A	100.0%	122.1 / NA	36	W205* (TGG→TGA)	yqiJ	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/22); total (14/22)

ACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTAT  >  NC_000913/3192691‑3192937

aCCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAat                                                                                                             <  2:170843/140‑1 (MQ=255)

            ggCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATttat                                                                                                 <  2:431139/140‑1 (MQ=255)

                       ttattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      <  2:226536/140‑1 (MQ=255)

                        tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGa                                                                                     >  2:581445/1‑140 (MQ=255)

                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  1:281579/1‑140 (MQ=255)

                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  2:286847/1‑140 (MQ=255)

                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  1:435546/1‑140 (MQ=255)

                               tactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaa                                                                              <  1:286847/140‑1 (MQ=255)

                                 ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                            <  2:281579/140‑1 (MQ=255)

                                 ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                            <  1:634820/140‑1 (MQ=255)

                                    gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:294227/1‑140 (MQ=255)

                                    gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:704673/1‑140 (MQ=255)

                                    gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:698535/1‑140 (MQ=255)

                                    gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGCAAATTTAATAATg                                                                         >  2:394303/1‑140 (MQ=255)

                                      gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      >  1:22067/1‑125 (MQ=255)

                                      gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      <  2:22067/125‑1 (MQ=255)

                                      gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGga                                                                       <  1:333446/140‑1 (MQ=255)

                                       ccTGAAGAAGAAAAAATTTT‑AATAAAAGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatg                                                                     <  1:391394/140‑1 (MQ=255)

                                          gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatgat                                                                   <  1:215156/140‑1 (MQ=255)

                                                      aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCt                                                       <  1:581445/140‑1 (MQ=255)

                                                       ttttCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATGCtg                                                      <  2:698535/140‑1 (MQ=255)

                                                           cACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                  <  1:516078/140‑1 (MQ=255)

                                                           cACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                  <  1:467866/140‑1 (MQ=255)

                                                             cTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcct                                                <  2:294227/140‑1 (MQ=255)

                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctcc                                              <  2:516438/140‑1 (MQ=255)

                                                                        aaaGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGttt                                     >  1:500794/1‑140 (MQ=255)

                                                                               tAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCaa                              >  2:755475/1‑140 (MQ=255)

                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                           >  2:643859/1‑136 (MQ=255)

                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                           <  1:643859/136‑1 (MQ=255)

                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                       <  2:421203/140‑1 (MQ=255)

                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                       <  1:295923/140‑1 (MQ=255)

                                                                                        gCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCg                     >  2:422419/1‑140 (MQ=255)

                                                                                            aCTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATg                 <  1:422419/140‑1 (MQ=255)

                                                                                                tCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCAtt             <  2:704673/140‑1 (MQ=255)

                                                                                                        gcgATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttat     >  1:26678/1‑140 (MQ=255)

                                                                                                           atatTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTtattat  <  2:26678/140‑1 (MQ=255)

ACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTAT  >  NC_000913/3192691‑3192937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A