Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F30 I1 R1	26	45.9	1554102	99.2%	1541669	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,437	G→T	K7N (AAG→AAT)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,437	0	G	T	100.0%	175.5 / NA	50	K7N (AAG→AAT)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (29/21); total (29/21)

AGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATG  >  NC_000913/3966306‑3966573

aGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGcc                                                                                                                                  <  2:121812/140‑1 (MQ=255)

aGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGcc                                                                                                                                  <  1:451632/140‑1 (MQ=255)

aGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGcc                                                                                                                                  >  1:150960/1‑140 (MQ=255)

  gCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCgg                                                                                                                                >  2:267408/1‑140 (MQ=255)

      cGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTc                                                                                                                            >  2:707074/1‑140 (MQ=255)

       gAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCt                                                                                                                           >  2:672017/1‑140 (MQ=255)

       gAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCt                                                                                                                           <  2:282333/140‑1 (MQ=255)

         aaaGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGa                                                                                                                         <  1:707074/140‑1 (MQ=255)

         aaaGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGa                                                                                                                         <  1:376592/140‑1 (MQ=255)

          aaGACATGATTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAg                                                                                                                        <  2:760861/140‑1 (MQ=255)

            gACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCt                                                                                                                      >  1:513232/1‑140 (MQ=255)

                     cGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCActct                                                                                                             >  1:570692/1‑140 (MQ=255)

                                     gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgggg                                                                                             >  1:178904/1‑140 (MQ=255)

                                     gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgggg                                                                                             >  1:451185/1‑140 (MQ=255)

                                         ggATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTgg                                                                                         >  2:56983/1‑140 (MQ=255)

                                               tCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                                                                                   <  1:99429/140‑1 (MQ=255)

                                               tCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                                                                                   >  1:312782/1‑140 (MQ=255)

                                                    ccATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                                                                              <  1:218864/140‑1 (MQ=255)

                                                       tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGt                                                                           <  1:672017/140‑1 (MQ=255)

                                                         ccATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAt                                                                         >  1:36326/1‑140 (MQ=255)

                                                         ccATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAt                                                                         >  2:414806/1‑140 (MQ=255)

                                                            ttCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg                                                                      >  1:185328/1‑140 (MQ=255)

                                                                 aacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc                                                                 <  2:185328/140‑1 (MQ=255)

                                                                   caTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCG‑AAGCAgg                                                              >  1:307291/1‑140 (MQ=255)

                                                                             gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt                                                     >  1:239723/1‑140 (MQ=255)

                                                                             gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGACCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTATGCAGGACATTAttt                                                     >  2:34829/1‑140 (MQ=255)

                                                                              agaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAtttt                                                    >  1:525892/1‑140 (MQ=255)

                                                                              agaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAtttt                                                    >  1:453749/1‑140 (MQ=255)

                                                                                aTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTg                                                  <  1:267408/140‑1 (MQ=255)

                                                                                 tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGc                                                 <  2:525892/140‑1 (MQ=255)

                                                                                   tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCa                                               >  1:154062/1‑140 (MQ=255)

                                                                                      cccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATcc                                            >  1:615333/1‑140 (MQ=255)

                                                                                        cAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTg                                          <  2:239723/140‑1 (MQ=255)

                                                                                           gTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAag                                       >  2:356989/1‑140 (MQ=255)

                                                                                             ttAAGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAagca                                     <  2:150960/140‑1 (MQ=255)

                                                                                                aGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTg                                                  >  2:207200/1‑124 (MQ=255)

                                                                                                aGAACTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTg                                                  <  1:207200/124‑1 (MQ=255)

                                                                                                    cTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCa                              >  1:36032/1‑140 (MQ=255)

                                                                                                    cTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCa                              <  2:570692/140‑1 (MQ=255)

                                                                                                    cTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCa                              >  2:592729/1‑140 (MQ=255)

                                                                                                    cTCACACCACTATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCa                              <  2:615333/140‑1 (MQ=255)

                                                                                                              tATGAATCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCg                    <  2:513232/140‑1 (MQ=255)

                                                                                                                   aTCTTACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGat               >  1:217047/1‑140 (MQ=255)

                                                                                                                       tACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGa                >  1:650433/1‑135 (MQ=255)

                                                                                                                       tACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGa                <  2:650433/135‑1 (MQ=255)

                                                                                                                       tACCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCt           >  1:196782/1‑140 (MQ=255)

                                                                                                                        aCCGAATTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCtt          >  1:331299/1‑140 (MQ=255)

                                                                                                                            aaTTAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGt      >  2:202254/1‑140 (MQ=255)

                                                                                                                              ttAAATAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGa    <  1:414806/140‑1 (MQ=255)

                                                                                                                                aaaTAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATg  <  2:178904/140‑1 (MQ=255)

AGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATG  >  NC_000913/3966306‑3966573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A