Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A34 F30 I1 R1	17	31.2	1061324	99.4%	1054956	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,292,111	C→A	L100L (CTG→CTT)	ccmH ←	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,292,111	0	C	A	100.0%	69.0 / NA	21	L100L (CTG→CTT)	ccmH	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/11); total (10/11)

GCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGT  >  NC_000913/2291977‑2292233

gCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCTAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAgcgg                                                                                                                       >  1:244092/1‑140 (MQ=255)

  aCGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAgcggcg                                                                                                                     >  1:199198/1‑140 (MQ=255)

      ttACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTaa                                                                                                                 >  1:366846/1‑140 (MQ=255)

                               gAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTg                                                                                        >  1:52719/1‑140 (MQ=255)

                               gAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTg                                                                                        >  1:377128/1‑140 (MQ=255)

                                  aCGCTCCCGGCACCACGCGTCCCCGACGCCGCGGGGGGGCTTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCg                                                                                     <  1:365014/140‑1 (MQ=255)

                                    gCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTa                                                                                   >  1:324811/1‑140 (MQ=255)

                                       cccGGCCCCCCGCGTCCCCGCCGCCGCGCGCGGGCGTATATCACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAAcg                                                                                <  1:520555/139‑1 (MQ=255)

                                         cGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAAcgcg                                                                              <  1:330911/140‑1 (MQ=255)

                                          ggCCCCACGGGTCCCCGACGCCGCGAGCGGGCGTATATGCCCAAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  1:86750/140‑1 (MQ=255)

                                          gcccccccgcgTCCCCGCCGCGGCGGGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:466511/133‑1 (MQ=255)

                                          gcccccccgcgTCCCCGCCGCCGGGGGGGGGCTTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:155876/133‑1 (MQ=255)

                                          gcccccacGCGTACCCGGCCCGGCGGGGGGGCGTATATGACCCACCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:366846/136‑1 (MQ=255)

                                            ccccacGCGTCCCGGACGCCGCGGGCGGGCGTATATGCCCAAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGcca                                                                           <  1:257492/138‑1 (MQ=255)

                                              ccccgcgTCCCCGCCGCCGCGGGCGGGCGTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGccacc                                                                         <  2:283442/137‑1 (MQ=255)

                                                                                      aCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCa                                 >  2:394602/1‑140 (MQ=255)

                                                                                        cGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATc                               <  2:13475/140‑1 (MQ=255)

                                                                                        cGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATc                               <  2:96779/140‑1 (MQ=255)

                                                                                                         actactGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCCTCCTGCATCAGTTCATACACTTTCTg              >  2:63666/1‑140 (MQ=255)

                                                                                                                gcagcaCCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAgg       >  2:330095/1‑140 (MQ=255)

                                                                                                                     aCCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCTTATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt  >  1:196880/1‑140 (MQ=255)

GCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGT  >  NC_000913/2291977‑2292233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A