Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A34 F30 I1 R1	17	31.2	1061324	99.4%	1054956	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,524,711	G→T	G56G (GGC→GGA)	xapA ←	xanthosine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,524,711	0	G	T	100.0%	106.7 / NA	31	G56G (GGC→GGA)	xapA	xanthosine phosphorylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (18/13); total (18/13)

CGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCA  >  NC_000913/2524574‑2524842

cGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATcc                                                                                                                                   >  1:74263/1‑140 (MQ=255)

                      tCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAAtt                                                                                                             >  1:17625/1‑140 (MQ=255)

                       cATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTg                                                                                                            >  1:427492/1‑140 (MQ=255)

                         ttCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCg                                                                                                          >  1:487284/1‑140 (MQ=255)

                            cACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACa                                                                                                       <  1:525100/140‑1 (MQ=255)

                             aCGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  1:195885/140‑1 (MQ=255)

                             aCGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGAGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  1:297773/140‑1 (MQ=255)

                              cGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  2:350520/139‑1 (MQ=255)

                              cGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      >  1:350520/1‑139 (MQ=255)

                               gACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCg                                                                                                    >  1:30865/1‑140 (MQ=255)

                                    tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtctc                                                                                               >  1:144104/1‑140 (MQ=255)

                                    tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtctc                                                                                               >  1:374060/1‑140 (MQ=255)

                                    tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtc                                                                                                 >  1:335791/1‑138 (MQ=255)

                                    tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtc                                                                                                 <  2:335791/138‑1 (MQ=255)

                                        aGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATc                                                                                           <  2:487284/140‑1 (MQ=255)

                                                 cgcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGcc                                                                                  >  1:361813/1‑140 (MQ=255)

                                                 cgcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGcc                                                                                  >  1:117000/1‑140 (MQ=255)

                                                                atacCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCg                                                                   <  2:361813/140‑1 (MQ=255)

                                                                 tacCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCgg                                                                  <  2:292803/140‑1 (MQ=255)

                                                                    caccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAc                                                               >  1:68091/1‑140 (MQ=255)

                                                                    caccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAc                                                               >  2:307359/1‑140 (MQ=255)

                                                                     accGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAcc                                                              <  2:17625/140‑1 (MQ=255)

                                                                         gTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTaaa                                                          >  2:101166/1‑140 (MQ=255)

                                                                                       tGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCg                                            >  1:421112/1‑140 (MQ=255)

                                                                                                  tAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAAt                                 <  2:144104/140‑1 (MQ=255)

                                                                                                     cTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAg                              <  2:451705/140‑1 (MQ=255)

                                                                                                                     tGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat              <  2:421112/140‑1 (MQ=255)

                                                                                                                           gTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATc        >  2:247091/1‑140 (MQ=255)

                                                                                                                                 aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  >  2:485870/1‑140 (MQ=255)

                                                                                                                                 aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  >  1:367912/1‑140 (MQ=255)

                                                                                                                                 aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  <  2:74263/140‑1 (MQ=255)

CGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCA  >  NC_000913/2524574‑2524842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A