Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F30 I1 R1	22	89.0	3054638	99.3%	3033255	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,592,222	C→A	G35V (GGG→GTG)	ugpB ←	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,592,222	0	C	A	98.8%	288.7 / ‑5.8	84	G35V (GGG→GTG)	ugpB	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (37/46); minor base G (0/1); total (37/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCCCTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATGCGCGAA  >  NC_000913/3592087‑3592358

ttGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTc                                                                                                                                      <  2:190492/140‑1 (MQ=255)

ttGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTc                                                                                                                                      <  1:173311/140‑1 (MQ=255)

 tGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTcc                                                                                                                                     >  1:1027327/1‑140 (MQ=255)

     ggTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAg                                                                                                                                 <  2:673433/140‑1 (MQ=255)

            aaTGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCGCTTCCATAGAATGCCa                                                                                                                          <  2:1464618/140‑1 (MQ=255)

                cGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAAc                                                                                                                      >  2:697921/1‑140 (MQ=255)

                 ggCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACg                                                                                                                     >  2:452418/1‑140 (MQ=255)

                     aTCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAAt                                                                                                                 >  2:1221053/1‑140 (MQ=255)

                      tCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATg                                                                                                                >  2:965949/1‑140 (MQ=255)

                      tCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATg                                                                                                                <  2:525116/140‑1 (MQ=255)

                       ccccGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATgg                                                                                                               <  2:732736/140‑1 (MQ=255)

                        cccGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgt                                                                                                              >  1:1274651/1‑140 (MQ=255)

                          cgcgCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcg                                                                                                            >  2:962359/1‑140 (MQ=255)

                          cgcgCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcg                                                                                                            >  2:530093/1‑140 (MQ=255)

                          cgcgCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcg                                                                                                            >  1:367431/1‑140 (MQ=255)

                            cgcTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcgtc                                                                                                          <  2:305982/140‑1 (MQ=255)

                             gcTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCTCCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCa                                                                                                         <  2:648212/140‑1 (MQ=255)

                              cTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCAc                                                                                                        <  1:1126183/140‑1 (MQ=255)

                              cTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACATCCATAGAATGCCAGAACGGAATGGTCGTCAc                                                                                                        <  2:745874/140‑1 (MQ=255)

                                       tGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATgg                                                                                                               >  2:394909/1‑124 (MQ=255)

                                       tGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATgg                                                                                                               <  1:394909/124‑1 (MQ=255)

                                         ttCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCATCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCAt                                                                                             >  2:1067070/1‑140 (MQ=255)

                                         ttCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATGGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCAt                                                                                             >  1:1426877/1‑140 (MQ=255)

                                         ttCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcgtc                                                                                                          >  1:151239/1‑127 (MQ=255)

                                         ttCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGgtcgtc                                                                                                          <  2:151239/127‑1 (MQ=255)

                                         ttCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCAt                                                                                             >  1:1306272/1‑140 (MQ=255)

                                            gTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCATAACGGAATGGTCGTCACTGCCTGTGCATTcc                                                                                          <  2:334930/140‑1 (MQ=255)

                                            gTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTcc                                                                                          <  2:182016/140‑1 (MQ=255)

                                                ttGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCAt                                                                                      <  1:1230130/140‑1 (MQ=255)

                                                       tataGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGcc                                                                               >  2:1229104/1‑140 (MQ=255)

                                                            gTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTcc                                                                          >  1:657024/1‑140 (MQ=255)

                                                            gTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTcc                                                                          >  1:742328/1‑140 (MQ=255)

                                                             tCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCg                                                                         >  2:903562/1‑140 (MQ=255)

                                                             tCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCg                                                                         >  2:1338994/1‑140 (MQ=255)

                                                              cGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGa                                                                        >  2:72769/1‑140 (MQ=255)

                                                               ggTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAg                                                                       >  1:53123/1‑140 (MQ=255)

                                                                gTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgc                                                                      <  2:657024/140‑1 (MQ=255)

                                                                 tACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcg                                                                     >  2:576500/1‑140 (MQ=255)

                                                                 tACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcg                                                                     >  1:363885/1‑140 (MQ=255)

                                                                 tACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcg                                                                     >  2:62462/1‑140 (MQ=255)

                                                                 tACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcg                                                                     >  1:396502/1‑140 (MQ=255)

                                                                 tACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcg                                                                     >  2:189754/1‑140 (MQ=255)

                                                                  aCAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAgcgc                                                                    <  1:698295/140‑1 (MQ=255)

                                                                   cAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGcc                                                                   <  1:434798/140‑1 (MQ=255)

                                                                    aaTTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCa                                                                  <  2:396502/140‑1 (MQ=255)

                                                                    aaTTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCa                                                                  <  2:1495809/140‑1 (MQ=255)

                                                                        ttGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGc                                                              <  1:1211141/140‑1 (MQ=255)

                                                                          gTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTg                                                            <  1:1338994/140‑1 (MQ=255)

                                                                           tAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGa                                                           >  2:1469252/1‑140 (MQ=255)

                                                                           tAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAAGGCCAGAACGGAATGGCCGCCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGACCGCCAGAGCGGa                                                           >  2:1427472/1‑140 (MQ=255)

                                                                            aaTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGaa                                                          <  1:189754/140‑1 (MQ=255)

                                                                            aaTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGaa                                                          <  1:576500/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:965949/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:1253532/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:1159887/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:697921/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:770335/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  2:1306272/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:1221053/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  1:903562/140‑1 (MQ=255)

                                                                             aTCCGGGTTTTCGGCGTTAAAACGATGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAg                                                         <  2:1274651/140‑1 (MQ=255)

                                                                                cGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGc                                                              >  2:1174169/1‑132 (MQ=255)

                                                                                cGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGc                                                              <  1:1174169/132‑1 (MQ=255)

                                                                                cGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTg                                                      >  1:21652/1‑140 (MQ=255)

                                                                                  ggTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGta                                                    <  2:1006120/140‑1 (MQ=255)

                                                                                         ggCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTa                                             <  1:72769/140‑1 (MQ=255)

                                                                                              tAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGt                                        >  2:1228333/1‑140 (MQ=255)

                                                                                                 aaCGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTc                                     <  1:62462/140‑1 (MQ=255)

                                                                                                        ggCCAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGttt                              <  2:1240022/140‑1 (MQ=255)

                                                                                                          ccAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAt                            <  2:367431/140‑1 (MQ=255)

                                                                                                          ccAGAGAATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAt                            <  1:1427472/140‑1 (MQ=255)

                                                                                                             gagaATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAtctc                         <  2:742328/140‑1 (MQ=255)

                                                                                                             gagaATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAtctc                         <  1:1228333/140‑1 (MQ=255)

                                                                                                             gagaATCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAtctc                         <  2:53123/140‑1 (MQ=255)

                                                                                                                 aTCCACCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCtt                     <  2:363885/140‑1 (MQ=255)

                                                                                                                     aCCTCTTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCttgttg                 >  2:10863/1‑140 (MQ=255)

                                                                                                                       ctctTTACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTa               <  1:1469252/140‑1 (MQ=255)

                                                                                                                          tttACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAtctc                         >  2:825849/1‑127 (MQ=255)

                                                                                                                          tttACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTAtctc                         <  1:825849/127‑1 (MQ=255)

                                                                                                                           ttACCCAGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGa           <  1:1229104/140‑1 (MQ=255)

                                                                                                                                 aGTTCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATgcgc     >  2:544698/1‑140 (MQ=255)

                                                                                                                                   ttCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATGCGCGa   >  2:283534/1‑140 (MQ=255)

                                                                                                                                   ttCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATGCGCGa   >  1:551798/1‑140 (MQ=255)

                                                                                                                                    tCCACTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATGCGCGaa  >  2:203168/1‑140 (MQ=255)

TTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAGAGAATCCACCTCTTTACCCAGTTCCCCTTCCATAGAATGCCAGAACGGAATGGTCGTCACTGCCTGTGCATTCCCCATTAACGCCAGTCCGAGCGCCAGTGCTGAAGCTGTATAATGTAACGGTTTCATCGTTTATCTCTCTTGTTGTACCGAATGCGCGAA  >  NC_000913/3592087‑3592358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A