Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,623	C→A	T141T (ACC→ACA)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,623	0	C	A	100.0%	110.4 / NA	33	T141T (ACC→ACA)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/16); total (17/16)

ATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCC  >  NC_000913/2601489‑2601758

aTCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTcgc                                                                                                                                    <  2:55718/140‑1 (MQ=255)

 tCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTcgcg                                                                                                                                   <  2:55922/140‑1 (MQ=255)

             gAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGAc                                                                                                                       >  1:149453/1‑140 (MQ=255)

                    tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAATGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:522260/1‑140 (MQ=255)

                    tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:552865/1‑140 (MQ=255)

                    tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:53546/1‑140 (MQ=255)

                    tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:343161/1‑140 (MQ=255)

                                  aTGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGaa                                                                                                  >  2:436460/1‑140 (MQ=255)

                                          tatGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGc                                                                                          >  2:27842/1‑140 (MQ=255)

                                                 ttCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCt                                                                                        >  2:65683/1‑135 (MQ=255)

                                                 ttCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCt                                                                                        <  1:65683/135‑1 (MQ=255)

                                                     ggCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTcgcg                                                                               <  2:149453/140‑1 (MQ=255)

                                                     ggCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTcgcg                                                                               <  1:27842/140‑1 (MQ=255)

                                                            ggCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGccc                                                                        <  1:359217/140‑1 (MQ=255)

                                                                 actcaAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATc                                                                   >  2:215206/1‑140 (MQ=255)

                                                                       aGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGt                                                             <  1:436460/140‑1 (MQ=255)

                                                                            gCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACgg                                                        <  1:123828/140‑1 (MQ=255)

                                                                                agaaAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGa                                                    <  1:522260/140‑1 (MQ=255)

                                                                                             acacaacaTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAg                                       <  1:215206/140‑1 (MQ=255)

                                                                                                 aacaTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTg                                   <  1:9500/140‑1 (MQ=255)

                                                                                                   caTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCa                                 >  1:307821/1‑140 (MQ=255)

                                                                                                       cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTg                                           >  2:378914/1‑126 (MQ=255)

                                                                                                       cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTg                                           <  1:378914/126‑1 (MQ=255)

                                                                                                       cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGt                             >  2:562566/1‑140 (MQ=255)

                                                                                                         ttGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCa                           <  1:53546/140‑1 (MQ=255)

                                                                                                           gctgcGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGa                         >  2:674219/1‑140 (MQ=255)

                                                                                                            ctgcGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGAt                        <  2:676919/140‑1 (MQ=255)

                                                                                                            ctgcGCTGGGAACCTGGTGGCCAGACAGTCGCTGTGAAATGGGACCTGTGTGATTTCTTGCCAGAAGGTCGGGCTTGCGTTCGCGCTTGCCAGAATCAGGCGTTGCGGCTGATTTCCGGTGATAGCCGGCAACGTCAGAt                        <  2:311180/140‑1 (MQ=255)

                                                                                                               cgcTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATgaa                     >  1:451751/1‑140 (MQ=255)

                                                                                                                            gtgtCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAgcgc        >  1:150751/1‑140 (MQ=255)

                                                                                                                             tgtCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGcc       <  1:674219/140‑1 (MQ=255)

                                                                                                                                 cAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCt      >  2:156446/1‑137 (MQ=255)

                                                                                                                                 cAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCt      <  1:156446/137‑1 (MQ=255)

                                                                                                                                  aGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGcc  >  1:393459/1‑140 (MQ=255)

ATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCC  >  NC_000913/2601489‑2601758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A