Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,715,561	G→T	F278L (TTC→TTA)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,715,561	0	G	T	100.0%	81.8 / NA	25	F278L (TTC→TTA)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/12); total (13/12)

CTGCCATCAGCATGGTGGTATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTCCATTTT  >  NC_000913/3715425‑3715692

cTGCCATCAGCATGGTGGTATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTTGCCCAGCAAATAAGAGGCTAAg                                                                                                                                  <  1:303799/140‑1 (MQ=255)

cTGCCATCAGCATGGTGGTATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAg                                                                                                                                  <  1:44105/140‑1 (MQ=255)

         gCATGGTGGTATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATaa                                                                                                                         >  2:703703/1‑140 (MQ=255)

                  tATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTTGCTATTTCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTgtggtg                                                                                                                >  1:486506/1‑140 (MQ=255)

                            gAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGGTTTCGCTATTCCGTCACTCTCGCCCAACAAATAAGAAGCCAAGACGGCACAAACTGTAGTGCAACGccccc                                                                                                      >  2:547081/1‑139 (MQ=255)

                                    ccgccAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTATGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCATCGCGCCAGAAACGCt                                                                                              <  2:492596/140‑1 (MQ=255)

                                        cAGCACGCGGTTTTTGGCTGCGCTAACACCACAAAGCTCTGCTGCTCATTCGGCAGTTTTCGCTATTCCGTCTCTCTCGCCCAGCAAATAAGTGGCTAAGACGGAATAACTTGTGGTGCAACGCGCCAGAAACGCTtcgt                                                                                          <  2:600654/140‑1 (MQ=255)

                                               cgGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCt                                                                                              >  1:409801/1‑129 (MQ=255)

                                               cgGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCt                                                                                              <  2:409801/129‑1 (MQ=255)

                                                 gATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAAc                                                                                 <  2:384998/140‑1 (MQ=255)

                                                                               tgctgcCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATcccc                                                    >  1:50181/1‑139 (MQ=255)

                                                                               tgctgcCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATcccc                                                    <  2:50181/139‑1 (MQ=255)

                                                                               tgctgcCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCa                                                   >  2:439483/1‑140 (MQ=255)

                                                                                    cccATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCAt                                               <  1:517154/139‑1 (MQ=255)

                                                                                    cccATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCAt                                               >  2:517154/1‑139 (MQ=255)

                                                                                                            cGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCAtgtg                       >  2:304810/1‑139 (MQ=255)

                                                                                                            cGTCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCAtgtg                       <  1:304810/139‑1 (MQ=255)

                                                                                                              tCACTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCgg                    >  1:170047/1‑140 (MQ=255)

                                                                                                                tctcaCGCCCAGCAAAGAAGGGGCTAAGGTGGCATAACCTATGGTGCAACGCGCCAGAAGCGGTGCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACAGCGGTGCCCATGTGCGGTg                  <  2:133581/139‑1 (MQ=255)

                                                                                                                aCTCTCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTg                  <  2:170047/140‑1 (MQ=255)

                                                                                                                 ctctCGCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCACAAACGCTTCGTCGTGCCAACCATTTTCCACCCGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGc                 >  2:24660/1‑140 (MQ=255)

                                                                                                                      gCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGcc                >  2:624480/1‑136 (MQ=255)

                                                                                                                      gCCCAGCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGcc                <  1:624480/136‑1 (MQ=255)

                                                                                                                           gCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCAtgtg                       >  1:561198/1‑124 (MQ=255)

                                                                                                                           gCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCAtgtg                       <  2:561198/124‑1 (MQ=255)

                                                                                                                           gCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGccacccac          <  1:436745/137‑1 (MQ=255)

                                                                                                                           gCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGccacccac          >  2:436745/1‑137 (MQ=255)

                                                                                                                           gCAAATAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTcc       >  2:488028/1‑140 (MQ=255)

                                                                                                                                tAAGAGGCTAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTCCAtttt  >  2:394174/1‑140 (MQ=255)

CTGCCATCAGCATGGTGGTATTTTGGTGGAAAATAGCCGCCAGCTCGCGGATTTTCGCTGCGCCAACACCACAAATCTCTGCTGCCCATTCGGCGGTTTTCGCTATTCCGTCACTCTCGCCCAGCAAATAAGAGGCGAAGACGGCATAACCTGTGGTGCAACGCGCCAGAAACGCTTCGTCGTGCCAACCATTTTCCACCAGCGTATGGGCGATCCCCAGCATCAGCGCAACATCGGTGCCCATGTGCGGTGCCACCCACTCCATTTT  >  NC_000913/3715425‑3715692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A