Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,218,455	C→A	intergenic (+42/‑141)	aceA → / → aceK	isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,218,455	0	C	A	100.0%	180.6 / NA	52	intergenic (+42/‑141)	aceA/aceK	isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (29/23); total (29/23)

TACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGG  >  NC_000913/4218320‑4218588

tACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAAcgc                                                                                                                                   >  1:46461/1‑140 (MQ=255)

          ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                                         >  2:215290/1‑140 (MQ=255)

             gATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                      <  1:109746/140‑1 (MQ=255)

             gATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                      <  2:559097/140‑1 (MQ=255)

               tAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCg                                                                                                                    >  2:480167/1‑140 (MQ=255)

               tAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCg                                                                                                                    >  2:507457/1‑140 (MQ=255)

                  aGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCgg                                                                                                                   >  2:637297/1‑138 (MQ=255)

                  aGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCgg                                                                                                                   <  1:637297/138‑1 (MQ=255)

                      acgacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACg                                                                                                             <  1:480167/140‑1 (MQ=255)

                        gacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGAt                                                                                                           >  2:723408/1‑140 (MQ=255)

                         acTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                      <  1:251065/128‑1 (MQ=255)

                         acTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                      >  2:251065/1‑128 (MQ=255)

                          cTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGc                                                                                                         <  1:507457/140‑1 (MQ=255)

                           tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGcc                                                                                                        >  2:456327/1‑140 (MQ=255)

                            attattCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                                                                       <  2:377670/140‑1 (MQ=255)

                              tattCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGa                                                                                                     >  1:486476/1‑140 (MQ=255)

                                     ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACg                                                                                                             >  2:60741/1‑125 (MQ=255)

                                     ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACg                                                                                                             <  1:60741/125‑1 (MQ=255)

                                     ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACg                                                                                              >  2:72067/1‑140 (MQ=255)

                                     ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACg                                                                                              >  2:504287/1‑140 (MQ=255)

                                     ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACg                                                                                              >  2:675387/1‑140 (MQ=255)

                                       cggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCt                                                                                            >  2:7507/1‑140 (MQ=255)

                                          cACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgc                                                                                         >  1:503884/1‑140 (MQ=255)

                                          cACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgc                                                                                         >  2:712010/1‑140 (MQ=255)

                                          cACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgc                                                                                         >  1:313998/1‑140 (MQ=255)

                                             gTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGcgcg                                                                                      <  1:291772/140‑1 (MQ=255)

                                               cTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTc                                                                                    <  1:72067/140‑1 (MQ=255)

                                                         cgcgCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgc                                                                                         >  2:562161/1‑125 (MQ=255)

                                                         cgcgCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgc                                                                                         <  1:562161/125‑1 (MQ=255)

                                                         cgcgCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                                                          >  2:371684/1‑140 (MQ=255)

                                                         cgcgCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                                                          >  1:720972/1‑140 (MQ=255)

                                                          gcgcTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGACAATCGGTGCACGAGGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCt                                                                         <  2:367491/140‑1 (MQ=25)

                                                           cgcTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTa                                                                        >  2:648726/1‑140 (MQ=255)

                                                           cgcTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTa                                                                        <  1:456327/140‑1 (MQ=255)

                                                           cgcTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTa                                                                        <  1:215290/140‑1 (MQ=255)

                                                                 ccGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCg                                                                  <  2:46461/140‑1 (MQ=255)

                                                                     cTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                              <  1:648726/140‑1 (MQ=255)

                                                                          cTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                              >  2:667899/1‑135 (MQ=255)

                                                                          cTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                              <  1:667899/135‑1 (MQ=255)

                                                                               gaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAgg                                                    >  1:223510/1‑140 (MQ=255)

                                                                                     cAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt                                              <  1:371684/140‑1 (MQ=255)

                                                                                       gTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATaa                                            <  1:7507/140‑1 (MQ=255)

                                                                                                  caacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTAcgc                                 >  1:166576/1‑140 (MQ=255)

                                                                                                    acaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt                                              <  2:537348/125‑1 (MQ=255)

                                                                                                    acaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt                                              >  1:537348/1‑125 (MQ=255)

                                                                                                         cGTTGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATcc                          >  2:529513/1‑140 (MQ=255)

                                                                                                           ttGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCgg                        >  1:639289/1‑140 (MQ=255)

                                                                                                            tGCTGACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGc                       >  1:415660/1‑140 (MQ=255)

                                                                                                                gACTGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAtt                   <  2:720972/140‑1 (MQ=255)

                                                                                                                             aTAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCTTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtgat      <  1:479757/140‑1 (MQ=255)

                                                                                                                             aTAAGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtgat      >  2:185280/1‑140 (MQ=255)

                                                                                                                               aaGGCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtgatga    <  2:29552/140‑1 (MQ=255)

                                                                                                                                 ggCGTTAACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAgg  <  2:313998/140‑1 (MQ=255)

TACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGG  >  NC_000913/4218320‑4218588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A