Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,221,548	C→A	V314F (GTT→TTT)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,221,548	0	C	A	96.4%	186.5 / 0.4	55	V314F (GTT→TTT)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (20/33); total (21/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTA  >  NC_000913/4221412‑4221681

aGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAga                                                                                                                                    <  2:674154/140‑1 (MQ=255)

     cGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAgattgat                                                                                                                               >  2:412946/1‑140 (MQ=255)

      gTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAgattgatt                                                                                                                              <  2:696397/140‑1 (MQ=255)

             ggTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACa                                                                                                                       >  1:142835/1‑140 (MQ=255)

               tGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATg                                                                                                                     <  2:142835/140‑1 (MQ=255)

                 aTTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGAt                                                                                                                   >  1:502436/1‑140 (MQ=255)

                  ttCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATc                                                                                                                  >  2:569133/1‑140 (MQ=255)

                     tGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACTTGATCAt                                                                                                                <  1:156466/139‑1 (MQ=255)

                     tGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACTTGATCAt                                                                                                                >  2:156466/1‑139 (MQ=255)

                       aCCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTg                                                                                                             <  1:412946/140‑1 (MQ=255)

                         cAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGAc                                                                                                           <  2:657155/140‑1 (MQ=255)

                               ccATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTc                                                                                                     >  1:376701/1‑140 (MQ=255)

                                  ttAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCagg                                                                                                  <  2:228597/140‑1 (MQ=255)

                                   tAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCagga                                                                                                 <  2:475592/140‑1 (MQ=255)

                                       aaaTTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCt                                                                                             <  2:675879/140‑1 (MQ=255)

                                          ttCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCtata                                                                                          >  1:430686/1‑140 (MQ=255)

                                            cATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        >  1:284392/1‑140 (MQ=255)

                                               cGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTa                                                                                     >  2:166320/1‑140 (MQ=255)

                                                         gATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATaaa                                                                           >  1:31736/1‑140 (MQ=255)

                                                          aTAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATaaaa                                                                          <  2:376701/140‑1 (MQ=255)

                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        <  1:405998/124‑1 (MQ=255)

                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        >  2:405998/1‑124 (MQ=255)

                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATc                                                                        >  1:613917/1‑140 (MQ=255)

                                                                 aGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCa                                                                   >  2:218563/1‑140 (MQ=255)

                                                                 aGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCa                                                                   <  1:205029/140‑1 (MQ=255)

                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATATTCTACATTCATAAAATCCa                                                                      >  2:99705/1‑135 (MQ=255)

                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCa                                                                      <  1:651325/135‑1 (MQ=255)

                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCa                                                                      >  2:651325/1‑135 (MQ=255)

                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCaga                                                                 <  1:218563/140‑1 (MQ=255)

                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAAAAACATGATCATTTGACCATTTCAGGAGGCTATATTCTACATTCATAAAATCCa                                                                      <  1:99705/135‑1 (MQ=255)

                                                                      ttATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGaa                                                              <  2:488730/140‑1 (MQ=255)

                                                                        aTTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            <  1:700067/140‑1 (MQ=255)

                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  2:267427/140‑1 (MQ=255)

                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  1:301963/140‑1 (MQ=255)

                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  2:414014/140‑1 (MQ=255)

                                                                            cATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgtgt                                                        <  2:635988/140‑1 (MQ=255)

                                                                             aTCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGtt                                                       <  1:378566/140‑1 (MQ=255)

                                                                                     aTCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            >  2:407450/1‑127 (MQ=255)

                                                                                     aTCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            <  1:407450/127‑1 (MQ=255)

                                                                                        cTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTctct                                            <  2:570321/140‑1 (MQ=255)

                                                                                           ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTg                                         >  2:301255/1‑140 (MQ=255)

                                                                                             aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCt                                       >  2:386545/1‑140 (MQ=255)

                                                                                             aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCt                                       <  1:479430/140‑1 (MQ=255)

                                                                                               cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCttt                                     >  2:54471/1‑140 (MQ=255)

                                                                                                aTCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTc                                    <  2:430686/140‑1 (MQ=255)

                                                                                                         tGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAAt                           >  1:61049/1‑140 (MQ=255)

                                                                                                         tGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAAt                           <  2:30634/140‑1 (MQ=255)

                                                                                                             cTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCtt                       >  1:183708/1‑140 (MQ=255)

                                                                                                                       tGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgt             <  1:54471/140‑1 (MQ=255)

                                                                                                                          tGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttag          <  2:61049/140‑1 (MQ=255)

                                                                                                                          tGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttag          <  2:613917/140‑1 (MQ=255)

                                                                                                                           ggAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttagt         <  2:284392/140‑1 (MQ=255)

                                                                                                                                tattGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACAt    <  1:597464/140‑1 (MQ=255)

                                                                                                                                  ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTa  <  2:183708/140‑1 (MQ=255)

                                                                                                                                  ttGCAAAAGATTGATTATTAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTa  <  2:403865/140‑1 (MQ=255)

AGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTA  >  NC_000913/4221412‑4221681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A