Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A32 F30 I1 R1 30 51.0 1723162 99.3% 1711099 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,382,280 G→T A13A (GCC→GCA)  frdA ← fumarate reductase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,382,2800GT100.0% 213.7 / NA 60A13A (GCC→GCA) frdAfumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/31);  total (29/31)

TGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATA  >  NC_000913/4382146‑4382415
                                                                                                                                      |                                                                                                                                       
tGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGcc                                                                                                                                    <  1:358756/140‑1 (MQ=255)
 gcgcGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCt                                                                                                                                   >  1:12148/1‑140 (MQ=255)
 gcgcGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCt                                                                                                                                   >  2:557966/1‑140 (MQ=255)
   gcgACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTAc                                                                                                                                 >  2:817467/1‑140 (MQ=255)
       cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  1:77950/1‑140 (MQ=255)
       cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  1:373034/1‑140 (MQ=255)
       cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  2:70001/1‑140 (MQ=255)
            gcggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAg                                                                                                                        >  1:430467/1‑140 (MQ=255)
               gAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     <  2:175827/140‑1 (MQ=255)
                 gCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCgg                                                                                                                   >  1:485957/1‑140 (MQ=255)
                       ttCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGaa                                                                                                             <  1:606665/140‑1 (MQ=255)
                           gcagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGt                                                                                                         <  2:294487/140‑1 (MQ=255)
                            cagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     <  2:513089/127‑1 (MQ=255)
                            cagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     >  1:513089/1‑127 (MQ=255)
                                 aCGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCAc                                                                                                   >  2:830154/1‑140 (MQ=255)
                                   ggTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGa                                                                                                 <  2:12148/140‑1 (MQ=255)
                                          cTACGCATC‑GGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATtcctc                                                                                         >  2:133462/1‑140 (MQ=255)
                                             cGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCa                                                                                       >  2:248900/1‑140 (MQ=255)
                                              gCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAg                                                                                      >  2:77818/1‑140 (MQ=255)
                                               cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  1:606367/140‑1 (MQ=255)
                                               cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  1:830154/140‑1 (MQ=255)
                                               cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  2:110146/140‑1 (MQ=255)
                                                aTCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGAt                                                                                    <  2:394704/140‑1 (MQ=255)
                                                             ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCca                                                                       >  1:651410/1‑140 (MQ=255)
                                                               gagaTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCcaca                                                                     >  2:348747/1‑140 (MQ=255)
                                                               gagaTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACCACATTCCTCCAGATTGTTTTTATCCcaca                                                                     >  2:58294/1‑140 (MQ=255)
                                                                  aTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGcc                                                                  >  2:365004/1‑140 (MQ=255)
                                                                    tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCAc                                                                <  1:557966/140‑1 (MQ=255)
                                                                    tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCAc                                                                <  2:688454/140‑1 (MQ=255)
                                                                       tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTa                                                             >  2:198321/1‑140 (MQ=255)
                                                                           aTTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTc                                                         <  2:624432/140‑1 (MQ=255)
                                                                            tttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCa                                                        >  2:448111/1‑140 (MQ=255)
                                                                               ttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAggg                                                     >  1:257029/1‑140 (MQ=255)
                                                                                tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                                                               >  1:570573/1‑129 (MQ=255)
                                                                                tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                                                               <  2:570573/129‑1 (MQ=255)
                                                                                tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGt                                                    <  2:454773/140‑1 (MQ=255)
                                                                                  cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTaa                                                  <  2:485957/140‑1 (MQ=255)
                                                                                   aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAg                                                 <  1:817467/140‑1 (MQ=255)
                                                                                            tGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCCCAGCCACGTACTTCAGGGGAAGTACCTGaaa                                        >  2:410438/1‑140 (MQ=255)
                                                                                               cTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGtt                                     <  2:430467/140‑1 (MQ=255)
                                                                                                tGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTa                                    <  1:627463/140‑1 (MQ=255)
                                                                                                   gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 >  1:39841/1‑140 (MQ=255)
                                                                                                   gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 <  1:77818/140‑1 (MQ=255)
                                                                                                   gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 >  1:167965/1‑140 (MQ=255)
                                                                                                    cgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGt                                <  1:70001/140‑1 (MQ=255)
                                                                                                             ttGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACg                       >  1:301439/1‑140 (MQ=255)
                                                                                                              tGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGc                      <  1:198321/140‑1 (MQ=255)
                                                                                                                 agcaCGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTAt                   <  2:39841/140‑1 (MQ=255)
                                                                                                                   caCGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTc                 >  1:820658/1‑140 (MQ=255)
                                                                                                                       tAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt             <  2:301439/140‑1 (MQ=255)
                                                                                                                        aaTCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTg            <  2:651410/140‑1 (MQ=255)
                                                                                                                         aTCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGc           <  1:133462/140‑1 (MQ=255)
                                                                                                                          tCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCTGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCt          >  1:344711/1‑140 (MQ=255)
                                                                                                                          tCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCt          <  2:820658/140‑1 (MQ=255)
                                                                                                                               cgcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc         <  2:191230/136‑1 (MQ=255)
                                                                                                                               cgcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc         >  1:191230/1‑136 (MQ=255)
                                                                                                                                gcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTta    <  1:24950/140‑1 (MQ=255)
                                                                                                                                gcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGCTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTta    >  2:99502/1‑140 (MQ=255)
                                                                                                                                 ccACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTtat   <  1:248900/140‑1 (MQ=255)
                                                                                                                                  cACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTtata  <  1:348747/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATA  >  NC_000913/4382146‑4382415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A