Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F30 I1 R1 23 155.7 5253390 99.4% 5221869 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,223,254 IS5 (–) +4 bp coding (622‑625/3093 nt) ebgA → evolved beta‑D‑galactosidase subunit alpha

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3130146 =NA (NA)160 (1.040) 99/274 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3223254 = 0 (0.000)coding (622/3093 nt) ebgA evolved beta‑D‑galactosidase subunit alpha
* ? NC_000913 = 3131340NA (NA)178 (1.150) 84/276 0.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3223257 0 (0.000)coding (625/3093 nt) ebgA evolved beta‑D‑galactosidase subunit alpha

Only 100 of 171 total aligned reads displayed.

GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3130285‑3130146
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGAT  >  NC_000913/3223254‑3223391
                                                                                                                                                                                                                                                                                      
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  1:251096/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  1:1145264/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  1:1976212/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  1:1858636/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  1:405189/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            >  2:1190013/1‑140
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC                                                                                                                                            <  1:1707882/140‑1
 AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCC                                                                                                                                           >  1:2132345/1‑140
  AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCT                                                                                                                                          >  2:1794579/1‑140
    ATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAA                                                                                                                                        >  1:2539562/1‑140
      CTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACG                                                                                                                                      <  2:2328468/140‑1
           TTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATAT                                                                                                                                 >  2:1107801/1‑140
            TCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATT                                                                                                                                <  2:1286856/140‑1
             CTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTA                                                                                                                               >  2:767056/1‑140
              TGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAA                                                                                                                              <  1:1998570/140‑1
               GGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAAC                                                                                                                             >  2:1870754/1‑140
                GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCT                                                                                                                                          >  1:661035/1‑126
                   TGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATT                                                                                                                         <  1:2201717/140‑1
                          GCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGT                                                                                                                  <  2:1910207/140‑1
                          GCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAA                                                                                                                              >  2:2009407/1‑128
                           CTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTG                                                                                                                 >  1:398179/1‑140
                               CTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTA                                                                                                             >  1:643035/1‑140
                                    GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGAC                                                                                                        <  2:418225/140‑1
                                     AATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACT                                                                                                       <  2:44371/140‑1
                                      ATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTGACGCATATTAACGATTTCACTGTGCGTACCGACTT                                                                                                      <  2:643035/140‑1
                                        TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTG                                                                                                    <  2:1129801/140‑1
                                        TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTG                                                                                                    >  1:608794/1‑140
                                          ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC                                                                                                  >  1:1321120/1‑140
                                          ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC                                                                                                  <  1:2131135/140‑1
                                          ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC                                                                                                  >  2:500092/1‑140
                                            TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC                                                                                                  >  2:1016444/1‑138
                                            TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAC                                                                                                  <  1:1016444/138‑1
                                            TGTAGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGA                                                                                                <  1:2243376/140‑1
                                               CGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGC                                                                                             >  1:1193848/1‑140
                                                 GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCT                                                                                           >  1:2267894/1‑140
                                                 GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCT                                                                                           >  1:1366833/1‑140
                                                 GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCT                                                                                           >  1:1730480/1‑140
                                                        TAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGA                                                                                    >  2:2067468/1‑140
                                                                 GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCT                                                                           >  2:1588649/1‑140
                                                                 GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCT                                                                           >  2:753201/1‑140
                                                                 GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCT                                                                           >  1:1631755/1‑140
                                                                  ACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTT                                                                          <  2:116309/140‑1
                                                                       TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTG                                                                     <  2:2060396/140‑1
                                                                         ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACG                                                                             <  1:630288/130‑1
                                                                            AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAG                                                                <  1:2465512/140‑1
                                                                            AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAG                                                                <  2:15817/140‑1
                                                                             ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGT                                                               >  1:2363392/1‑140
                                                                              CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTG                                                              >  2:737264/1‑140
                                                                              CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGCACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTG                                                              >  2:328358/1‑140
                                                                                 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTG                                                           >  2:1137879/1‑140
                                                                                 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTG                                                           >  2:1412102/1‑140
                                                                                 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTG                                                           >  2:2531109/1‑140
                                                                                 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTG                                                           >  1:2037311/1‑140
                                                                                 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTG                                                           <  1:328358/140‑1
                                                                                  GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGC                                                          >  1:970793/1‑140
                                                                                  GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGC                                                          >  1:2014034/1‑140
                                                                                   TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCT                                                         >  1:1883158/1‑140
                                                                                        TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAA                                                    >  1:2121881/1‑140
                                                                                        TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAA                                                    <  1:1107801/140‑1
                                                                                         GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAA                                                   >  2:115227/1‑140
                                                                                           CTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATC                                                 >  1:1812702/1‑140
                                                                                             CCAGATGTCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTC                                               >  2:425680/1‑140
                                                                                             CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTC                                               >  2:2540238/1‑140
                                                                                             CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTC                                               >  2:1917820/1‑140
                                                                                               AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGC                                             <  1:701051/140‑1
                                                                                                  TGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGC                                          <  1:1588649/140‑1
                                                                                                        ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCC                                    >  1:1645646/1‑140
                                                                                                          ATGGTCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTG                                  >  1:2049607/1‑140
                                                                                                                TCATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCA                            <  2:2049607/140‑1
                                                                                                                 CATATCAGGGACTTGTTCGCACCTTCCCTAGCGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCAC                           <  2:548326/140‑1
                                                                                                                 CATATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCAC                           <  1:2483418/140‑1
                                                                                                                   TATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGA                         <  2:1631755/140‑1
                                                                                                                   TATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGA                         <  2:1730480/140‑1
                                                                                                                    ATCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGAC                        >  1:810868/1‑140
                                                                                                                     TCAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACG                       <  2:696098/140‑1
                                                                                                                      CAGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGC                      <  1:2227256/140‑1
                                                                                                                       AGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCT                     <  2:387386/140‑1
                                                                                                                       AGGGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCT                     <  1:425680/140‑1
                                                                                                                         GGACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGG                   >  1:1098342/1‑140
                                                                                                                          GACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCT                     <  1:1508846/137‑1
                                                                                                                          GACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCT                     >  2:1508846/1‑137
                                                                                                                          GACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCT                     <  1:513173/137‑1
                                                                                                                           ACTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAA                 <  2:303856/140‑1
                                                                                                                            CTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAAT                >  1:230765/1‑140
                                                                                                                            CTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGC                      <  2:604882/134‑1
                                                                                                                            CTTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGC                      >  1:604882/1‑134
                                                                                                                             TTGTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATA               <  2:2363392/140‑1
                                                                                                                               GTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATA             <  2:532287/140‑1
                                                                                                                               GTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATA             >  2:652718/1‑140
                                                                                                                               GTTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATA             <  1:737264/140‑1
                                                                                                                                TTCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATAC            >  2:298932/1‑140
                                                                                                                                 TCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACC           <  2:1366833/140‑1
                                                                                                                                 TCGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACC           <  2:2267894/140‑1
                                                                                                                                  CGCACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCC          <  2:533297/140‑1
                                                                                                                                    CACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTG        >  1:1834297/1‑140
                                                                                                                                    CACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTG        >  2:886139/1‑140
                                                                                                                                     ACCTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGT       >  2:2471428/1‑140
                                                                                                                                       CTTCCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTT     >  1:768074/1‑140
                                                                                                                                          CCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGAT  >  2:2285469/1‑140
                                                                                                                                          CCCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATA               <  1:1149126/127‑1
                                                                                                                                                                                                                                                                                      
GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3130285‑3130146
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGAT  >  NC_000913/3223254‑3223391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A