Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F30 I1 R1	18	41.0	1418494	98.5%	1397216	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,366,690	C→A	H261N (CAC→AAC)	arnB →	UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,366,690	0	C	A	100.0%	155.4 / NA	46	H261N (CAC→AAC)	arnB	UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (29/17); total (29/17)

ACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATGAACA  >  NC_000913/2366553‑2366826

aCGGTCTGGGTGTCGATGCCTAAGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAAc                                                                                                                                        <  1:509724/140‑1 (MQ=255)

        ggTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAacac                                                                                                                                >  1:24787/1‑140 (MQ=255)

                tGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGgc                                                                                                                        >  2:692169/1‑140 (MQ=255)

                             caAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCa                                                                                                           <  1:383725/140‑1 (MQ=255)

                                aCCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGcc                                                                                                             >  2:688385/1‑135 (MQ=255)

                                aCCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGcc                                                                                                             <  1:688385/135‑1 (MQ=255)

                                           tGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAata                                                                                                     >  1:106285/1‑132 (MQ=255)

                                           tGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAata                                                                                                     <  2:106285/132‑1 (MQ=255)

                                            gCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCagcaagc                                                                                            >  2:415665/1‑140 (MQ=255)

                                              aCCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCAc                                                                                          <  2:83064/140‑1 (MQ=255)

                                                 gCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTgg                                                                                       <  2:79992/140‑1 (MQ=255)

                                                                      gggCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCAt                                                                  >  2:685790/1‑140 (MQ=255)

                                                                      gggCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCAt                                                                  >  1:674225/1‑140 (MQ=255)

                                                                       ggCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCAtt                                                                 >  2:339472/1‑140 (MQ=255)

                                                                            taAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGcc                                                            >  2:636503/1‑140 (MQ=255)

                                                                                 aCAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTccc                                                       >  1:138262/1‑140 (MQ=255)

                                                                                  cAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCg                                                      >  1:641212/1‑140 (MQ=255)

                                                                                   aaTCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGc                                                     <  2:24787/140‑1 (MQ=255)

                                                                                      cTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTg                                                  >  1:250415/1‑140 (MQ=255)

                                                                                      cTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTg                                                  >  2:29737/1‑140 (MQ=255)

                                                                                         aCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGcc                                               >  1:20276/1‑140 (MQ=255)

                                                                                         aCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGcc                                               >  2:502574/1‑140 (MQ=255)

                                                                                                ttAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGtt                                        <  1:15320/140‑1 (MQ=255)

                                                                                                      ccgcGATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGcc                                  <  1:685790/140‑1 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGtt                                        >  1:126419/1‑132 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGtt                                        <  2:126419/132‑1 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  1:585211/1‑140 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  1:570262/1‑140 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  2:103898/1‑140 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  1:109889/1‑140 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  2:260147/1‑140 (MQ=255)

                                                                                                        gcgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                                >  2:221865/1‑140 (MQ=255)

                                                                                                         cgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTgg                               >  1:270589/1‑140 (MQ=255)

                                                                                                         cgATTGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTgg                               <  2:20276/140‑1 (MQ=255)

                                                                                                            ttGCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                            <  1:636503/140‑1 (MQ=255)

                                                                                                              gCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt                          <  2:674225/140‑1 (MQ=255)

                                                                                                              gCCCTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt                          >  1:324491/1‑140 (MQ=255)

                                                                                                               cccTGACACAGTTAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTg                         >  1:367523/1‑140 (MQ=255)

                                                                                                                          ttAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg              >  1:97329/1‑140 (MQ=255)

                                                                                                                          ttAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg              >  1:124702/1‑140 (MQ=255)

                                                                                                                          ttAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg              <  1:260147/140‑1 (MQ=255)

                                                                                                                          ttAGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg              <  1:103898/140‑1 (MQ=255)

                                                                                                                            aGTCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCgtg            <  2:124702/140‑1 (MQ=255)

                                                                                                                              tCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTc          >  2:502864/1‑140 (MQ=255)

                                                                                                                              tCAAATTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTc          <  2:641212/140‑1 (MQ=255)

                                                                                                                                  aTTAGAGAACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATg      >  1:695992/1‑140 (MQ=255)

                                                                                                                                      gagaACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATGaaca  <  2:250415/140‑1 (MQ=255)

                                                                                                                                      gagaACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATGaaca  <  2:270589/140‑1 (MQ=255)

                                                                                                                                      gagaACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTAGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATGaaca  <  1:692169/140‑1 (MQ=255)

ACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCGTGTCGATGAACA  >  NC_000913/2366553‑2366826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A