Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F30 I1 R1	18	41.0	1418494	98.5%	1397216	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,074,162	T→G	A176A (GCA→GCC)	yggC ←	P‑loop NTPase domain‑containing protein YggC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,074,162	0	T	G	100.0%	154.1 / NA	42	A176A (GCA→GCC)	yggC	P‑loop NTPase domain‑containing protein YggC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/25); total (17/25)

TTGGCCTGACGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGTGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTT  >  NC_000913/3074025‑3074298

ttGGCCTGACGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGc                                                                                                                                        >  2:406072/1‑140 (MQ=255)

 tGGCCTGACGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCa                                                                                                                                       <  2:411025/140‑1 (MQ=255)

    ccTGACGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGa                                                                                                                                    >  1:627741/1‑140 (MQ=255)

        aCGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATaa                                                                                                                                >  2:647007/1‑140 (MQ=255)

             tATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATa                                                                                                                           >  1:241239/1‑140 (MQ=255)

                  atcaGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGaaaa                                                                                                                      <  1:525330/140‑1 (MQ=255)

                       cacaCGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGc                                                                                                                 >  2:443301/1‑140 (MQ=255)

                           cGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGaa                                                                                                             >  2:418119/1‑140 (MQ=255)

                                   aTTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCa                                                                                                     <  1:406072/140‑1 (MQ=255)

                                      ggggCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACt                                                                                                  >  1:466017/1‑140 (MQ=255)

                                           cATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGc                                                                                             <  2:643504/140‑1 (MQ=255)

                                           cATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGc                                                                                             <  2:466017/140‑1 (MQ=255)

                                            aTCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGcc                                                                                            >  1:499141/1‑140 (MQ=255)

                                            aTCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGcc                                                                                            >  1:252331/1‑140 (MQ=255)

                                             tCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCa                                                                                           <  2:120497/140‑1 (MQ=255)

                                                   cGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGGAGAAAGATGCCAACTCCAGCCACTTTtc                                                                                     >  2:614769/1‑140 (MQ=255)

                                                    gggCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTtca                                                                                    <  2:252331/140‑1 (MQ=255)

                                                    gggCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTtca                                                                                    <  2:627741/140‑1 (MQ=255)

                                                    gggCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTtca                                                                                    <  1:577247/140‑1 (MQ=255)

                                                     ggCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTtcat                                                                                   >  2:562429/1‑140 (MQ=255)

                                                         tAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCatcatc                                                                               <  2:234529/140‑1 (MQ=255)

                                                                cTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCatcatc                                                                               >  2:585767/1‑133 (MQ=255)

                                                                cTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCatcatc                                                                               <  1:585767/133‑1 (MQ=255)

                                                                cTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTaac                                                                        >  1:427830/1‑140 (MQ=255)

                                                                cTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTaac                                                                        >  2:438552/1‑140 (MQ=255)

                                                                 ttCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTaaca                                                                       >  1:34909/1‑140 (MQ=255)

                                                                      cTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAg                                                                  <  1:438552/140‑1 (MQ=255)

                                                                       tACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGt                                                                 <  2:333806/140‑1 (MQ=255)

                                                                                cgTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTcga                                                        >  2:476698/1‑140 (MQ=255)

                                                                                  ttAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTcgacg                                                      <  1:194918/140‑1 (MQ=255)

                                                                                  ttAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTcgacg                                                      <  2:241239/140‑1 (MQ=255)

                                                                                          cccGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAg                                              <  2:34909/140‑1 (MQ=255)

                                                                                          cccGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAg                                              <  2:562245/140‑1 (MQ=255)

                                                                                          cccGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAg                                              <  2:64085/140‑1 (MQ=255)

                                                                                          cccGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAg                                              <  1:418119/140‑1 (MQ=255)

                                                                                               aaTTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAgtggtg                                         <  2:499141/140‑1 (MQ=255)

                                                                                               aaTTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAgtggtg                                         <  1:580474/140‑1 (MQ=255)

                                                                                                    tACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTa                                    <  1:682893/140‑1 (MQ=255)

                                                                                                               aaGCGCTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGc                         <  2:427830/140‑1 (MQ=255)

                                                                                                                  cgcTCCCGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATc                      <  1:562429/140‑1 (MQ=255)

                                                                                                                      cccGCAGAATTTGAGCAGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCa                  >  1:341522/1‑140 (MQ=255)

                                                                                                                                      aGGGGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCtttt  <  1:683422/140‑1 (MQ=255)

TTGGCCTGACGGCTATTCATCAGCACACGCTCGACATTGGGGCCATCGGTGCGGGCATAAAATGCTTCGGCTACTTGCCGCGTTAATCCTCCCGCAATTTTACGACTAATCAAGCGCTCCCGCAGAATTTGAGCAGGTGCATGAATAAAAATAGAAAAATCGCAGAAAGATGCCAACTCCAGCCACTTTTCATCATCCAGTAACAACCAGTTTCCTTCGACGATGACGAGTGGTGCGGTAACGTGCAACGCATCTTCAACAGGATCATGCTTTT  >  NC_000913/3074025‑3074298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A