Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F30 I1 R1	19	47.3	1605240	99.2%	1592398	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,952,237	G→T	I75I (ATC→ATA)	recD ←	exodeoxyribonuclease V subunit RecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,952,237	0	G	T	100.0%	95.5 / NA	28	I75I (ATC→ATA)	recD	exodeoxyribonuclease V subunit RecD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/13); total (15/13)

CCACTGTGCGCTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCGATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGATGTTCA  >  NC_000913/2952104‑2952372

ccACTGTGCGCTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCa                                                                                                                                   >  2:577021/1‑140 (MQ=255)

 cACTGTGCGCTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCAc                                                                                                                                  <  1:386401/140‑1 (MQ=255)

    tgtgCGCTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGa                                                                                                                               >  2:30195/1‑140 (MQ=255)

        cgcTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCA‑CTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAg                                                                                                                          <  1:686023/140‑1 (MQ=255)

                             ttCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGa                                                                                                      <  1:252070/140‑1 (MQ=255)

                                    aaaGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCg                                                                                               <  1:269063/140‑1 (MQ=255)

                                                           atcGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCgg                                                                        >  2:38476/1‑140 (MQ=255)

                                                            tcGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGc                                                                       >  1:308324/1‑140 (MQ=255)

                                                                 gTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGcaaaca                                                                  >  1:620765/1‑140 (MQ=255)

                                                                  tGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGcaaacaa                                                                 <  1:577021/140‑1 (MQ=255)

                                                                          tctcCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTcc                                                         <  1:15728/140‑1 (MQ=255)

                                                                          tctcCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTcc                                                         <  1:690651/140‑1 (MQ=255)

                                                                              cccTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCctct                                                     >  1:149603/1‑140 (MQ=255)

                                                                               ccTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGcaaa                                                                    <  2:117667/124‑1 (MQ=255)

                                                                               ccTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGcaaa                                                                    >  1:117667/1‑124 (MQ=255)

                                                                                        cGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGcatcat                                           >  1:528356/1‑140 (MQ=255)

                                                                                        cGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGcatcat                                           >  2:87692/1‑140 (MQ=255)

                                                                                                       caagcaTTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCTGGCATCATGACTTAACAGTgccg                            >  2:60892/1‑140 (MQ=255)

                                                                                                       caagcaTTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTgccg                            >  2:668722/1‑140 (MQ=255)

                                                                                                            aTTCTTCCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCcgcg                       <  2:528356/140‑1 (MQ=255)

                                                                                                                 tcCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGt                  <  1:38476/140‑1 (MQ=255)

                                                                                                                 tcCCAATTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGt                  <  2:308324/140‑1 (MQ=255)

                                                                                                                     aaTTTTGTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACg              <  1:398473/140‑1 (MQ=255)

                                                                                                                           gTAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGa        <  1:87692/140‑1 (MQ=255)

                                                                                                                            tAGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGAt       >  2:707965/1‑140 (MQ=255)

                                                                                                                             aGCTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGATg      >  2:793698/1‑140 (MQ=255)

                                                                                                                               cTCACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGATGtt    >  1:157057/1‑140 (MQ=255)

                                                                                                                                 cACCTATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGATGTTCa  >  1:573053/1‑140 (MQ=255)

CCACTGTGCGCTCGTTACACCACATGCGATTCAAATAAAGACGATCGCCACAGAGGATCATCGGCGTGGGTTCATCTCCCCTGCTGACCGCTTGAGAAGCCAGCAAGCATTCTTCCCAATTTTGTAGCTCACCGATTTCACTGACACAGGTCGCCAACAGCGGATGCGACGCCTCGTTATTTTCCAGTCGTGAAAGCGGCAAACAAACGTGTCCCTCTCCGGCATCATGACTTAACAGTGCCGCCGCGAGGGTGACGGCAGGATGTTCA  >  NC_000913/2952104‑2952372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A