Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F30 I1 R1	18	107.8	3626394	99.4%	3604635	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,809,405	C→A	pseudogene (1090/1178 nt)	ygaY →	putative transporter YgaY, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,405	0	C	A	100.0%	305.4 / NA	87	pseudogene (1090/1178 nt)	ygaY	putative transporter YgaY, N‑terminal fragment
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/68); total (19/68)

Only 100 of 114 total aligned reads displayed.

CTAACCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGCTGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGA  >  NC_000913/2809270‑2809541

 tAACCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑Gggtg‑gggtgggg                                                                                                                                            >  1:1120465/1‑138 (MQ=255)

   aCCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTT‑GGGTgggggt                                                                                                                                          >  1:1505547/1‑136 (MQ=255)

   aCCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGTGGCggt                                                                                                                                          >  1:1323608/1‑138 (MQ=255)

        aCGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGTGGCGgtttttt                                                                                                                                     >  1:1118817/1‑136 (MQ=255)

           gTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTctgg‑c                                                                                                                                 <  1:103069/140‑1 (MQ=255)

                 tATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGgcgc                                                                                                                           <  1:1661699/140‑1 (MQ=255)

                 tATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGgcgc                                                                                                                           <  1:1418955/140‑1 (MQ=255)

                 tATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGgcgc                                                                                                                           <  1:813047/140‑1 (MQ=255)

                      gATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGc‑gac‑ga                                                                                                                    <  1:894844/140‑1 (MQ=255)

                      gATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGc‑gac‑ga                                                                                                                    <  1:416738/140‑1 (MQ=255)

                      gATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGc‑gac‑ga                                                                                                                    <  1:804028/140‑1 (MQ=255)

                      gATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTT‑GGGGGGCGGTTTTTTGG‑CTGGGgc‑ggc‑gc                                                                                                                    >  2:103069/1‑139 (MQ=255)

                         aCATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTg                                                                                                                <  1:1655860/140‑1 (MQ=255)

                          cATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGc                                                                                                               <  2:56848/140‑1 (MQ=255)

                          cATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGc                                                                                                               <  1:362915/140‑1 (MQ=255)

                           aTCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGcc                                                                                                              <  1:749289/140‑1 (MQ=255)

                           aTCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGcc                                                                                                              <  1:1025444/140‑1 (MQ=255)

                             ccTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑t                                                                                                           <  1:1538506/140‑1 (MQ=255)

                             ccTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑t                                                                                                           <  1:1757366/140‑1 (MQ=255)

                             ccTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑t                                                                                                           <  1:362057/140‑1 (MQ=255)

                               tGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑Tgg                                                                                                         <  1:1022846/140‑1 (MQ=255)

                                 aTGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGGTTG‑GGGGGGGGTTTTTTTGG‑CGGGGGG‑GCG‑GT‑TTTCCC‑TGGt                                                                                                        >  2:723371/1‑140 (MQ=255)

                                 aTGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCCCCGGGTTGCTTAATTCAGCCCCAGGCCGGCGACATGGC‑GGTTGTGGCTGG‑GCTTGTTTGGACGGGCGG‑CCC‑GG‑TTGCCC‑TGGt                                                                                                        >  2:727815/1‑140 (MQ=255)

                                  tGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTa                                                                                                      <  2:1526392/140‑1 (MQ=255)

                                  tGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTa                                                                                                      <  1:1321481/140‑1 (MQ=255)

                                    cgcgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGTGGGGGTTGTTTGG‑GTGGCGC‑GACGGA‑TTGCCC‑GGGTTaa                                                                                                     >  1:1288808/1‑140 (MQ=255)

                                     gcgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTTAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGCGGTTTTCTGG‑GTGGGGC‑GGCGGG‑TTGCCT‑GGGTTaat                                                                                                    >  1:266255/1‑139 (MQ=255)

                                     gcgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGGTTG‑GGGGGGGGTTTTTTTGG‑‑TGGGGC‑GGC‑GAGTTGCCC‑TGGTTaat                                                                                                    >  2:1538506/1‑139 (MQ=255)

                                      cgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGTCCCCGGCTCCCTAATTTCAACCTCATCCCTGCAACATGGT‑GGTTT‑GGCTGGGGTGTTTCTGT‑CTCGCGC‑CCG‑GC‑GTGCCCTTGGTTAACt                                                                                                   >  2:731362/1‑140 (MQ=255)

                                      cgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTTAGCCTCAGCCTGGCAACATGGCGGGTTG‑GGTGGGGGTTTTTTTGG‑‑TGGGGC‑GGC‑CG‑TTGGCC‑CGGTTTACtt                                                                                                  >  2:144181/1‑140 (MQ=255)

                                      cgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGCAAAATGGC‑GGGTG‑GGGTGGGGGTTTTTTTG‑GTGGGGC‑GGC‑GAGTTCCCC‑CGGTTaatt                                                                                                   >  2:1757366/1‑138 (MQ=255)

                                        cAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTAc                                                                                                <  1:253973/140‑1 (MQ=255)

                                              ccTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTActggtct                                                                                          <  1:723371/140‑1 (MQ=255)

                                                    acagggTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGc                                                                                    <  1:942493/136‑1 (MQ=255)

                                                     gagacagaCATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCg                                                                                   <  1:1186363/133‑1 (MQ=255)

                                                      cAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGa                                                                                  <  1:144181/140‑1 (MQ=255)

                                                             cATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTc                                                                           <  1:266074/140‑1 (MQ=255)

                                                                   tAGCTACTTTATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGGAACATTGC‑GGGTG‑GGGGGGCGTTTGTCTGG‑CTGGCGC‑GGC‑GG‑TTGCCC‑CGGGTAAATTTCTGGTCTGGGGGGGAGGGGTTTATCG‑Tc                                                                    >  2:335226/1‑140 (MQ=255)

                                                                     gCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑g                                                                 <  1:979994/140‑1 (MQ=255)

                                                                     gCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGGGGTTTTTTTG‑CTGGGGC‑GGC‑GG‑TTTCCC‑TGGGTTAATTTCTGGGCTTGTGGGGGGGGGTTTATCG‑Tccg                                                                  >  2:615237/1‑138 (MQ=255)

                                                                     gCTACTTTATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCCGCCTGGCAACATGGC‑GGGTG‑GGGGGGGGGTTTTTTGG‑GTGGCGC‑GGC‑GG‑TTTTCC‑CGGGTTACTTTCTGGGGTGGTGGCGGGGGTTTTATCG‑TCA‑g                                                                 >  2:266074/1‑140 (MQ=255)

                                                                      cTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑gg                                                                <  1:1557098/140‑1 (MQ=255)

                                                                       tACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGa                                                               <  1:412341/140‑1 (MQ=255)

                                                                           ttATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGcc                                                           <  1:937668/140‑1 (MQ=255)

                                                                           gtATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGct                                                           <  1:641088/139‑2 (MQ=255)

                                                                            tATTGGCGGTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTT‑GGGTGGGGGTTTTTTGG‑GTGGCGC‑GGC‑GA‑TTGCCC‑CGGGTTAATTTCTGGGCTGGGGGGGGGGGTTTTATCG‑TCC‑gggaaccc                                                          >  2:187450/1‑134 (MQ=255)

                                                                                   ggTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGGGGTTTTTTGG‑CTGGCGCGGCC‑GT‑TTCCCC‑TGG‑TAAATTTCTTGGCTGGGGGGGGGGGTTTCATCGTTCG‑GGAAG‑CGCAAAATa                                                   >  2:26842/1‑140 (MQ=255)

                                                                                   ggTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGGGGTTTTCTTG‑CTGGCGC‑GACGGA‑TTCCCC‑GGGTT‑ACTTTCTTGGCTGGGGGGGGGGGTTTTATCG‑TCA‑GGAAACCGCCAAATa                                                   >  2:1709026/1‑140 (MQ=255)

                                                                                   ggTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGCGGTTTTTTGG‑CTGGCGG‑GGC‑GAGTTGCCC‑GGGTTAA‑TTTTTGGGCTGGGGGGGGGGGTTTTATTG‑TCC‑GGGAGGCGCAAAATa                                                   >  2:1188990/1‑140 (MQ=255)

                                                                                    gTGCCGCCGGGTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGGTG‑GGGGGGGGGTTTTCTTG‑GTGGCGC‑GGC‑GA‑ATTCCC‑CGGGTTACTTTATGGGGTGGTGGGGGGGGTTTTCTTG‑TCAGGGAAGCCCCAAATTa                                                   >  2:614599/1‑140 (MQ=255)

                                                                                         gccgGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAAt                                             <  1:731362/140‑1 (MQ=255)

                                                                                           cgGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCa                                           >  2:246238/1‑140 (MQ=255)

                                                                                            ggTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGGGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAg                                          >  1:190776/1‑140 (MQ=255)

                                                                                              ttCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCa                                        >  2:150001/1‑140 (MQ=255)

                                                                                                cGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAAc                                      >  1:1528565/1‑140 (MQ=255)

                                                                                                 gCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAAcc                                     <  1:615237/140‑1 (MQ=255)

                                                                                                        tcagcctcagccTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCg                              <  1:335226/140‑1 (MQ=255)

                                                                                                        tcagcctcagccTGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCg                              <  1:448118/140‑1 (MQ=255)

                                                                                                              tcagcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCtt                        <  1:1345/140‑1 (MQ=255)

                                                                                                               cagcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCttt                       <  1:246238/140‑1 (MQ=255)

                                                                                                               cagcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCttt                       >  1:1111809/1‑140 (MQ=255)

                                                                                                               cagcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCttt                       >  1:754081/1‑140 (MQ=255)

                                                                                                               cagcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCttt                       >  1:60701/1‑140 (MQ=255)

                                                                                                               aggcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCttt                       <  1:452699/138‑1 (MQ=255)

                                                                                                                agcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGTGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTa                      <  1:325374/140‑1 (MQ=255)

                                                                                                                agcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTTCTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTa                      >  2:67529/1‑140 (MQ=255)

                                                                                                                agcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTa                      >  2:1150427/1‑140 (MQ=255)

                                                                                                                agcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTa                      >  2:600020/1‑140 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTTCTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:67529/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCtt                        <  1:1738118/137‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  2:1111809/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  2:1323608/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:1647464/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:150001/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:727815/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:653117/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:1188990/140‑1 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     >  2:1647464/1‑140 (MQ=255)

                                                                                                                 gcctGGCAACATGGC‑GGTTG‑GGATGGCGGTTGTCTGA‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGGGGCGAGGGTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGAAATCCGCAACCGTTTTCGTGGCtttcc                     >  2:1031209/1‑138 (MQ=255)

                                                                                                                      gCAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTta                >  1:1373831/1‑140 (MQ=255)

                                                                                                                       cAACATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTtat               >  1:712250/1‑140 (MQ=255)

                                                                                                                        aaCATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTtata              <  2:190776/140‑1 (MQ=255)

                                                                                                                        aaCATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTtata              <  1:654491/140‑1 (MQ=255)

                                                                                                                         aCATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATaa             <  1:600020/140‑1 (MQ=255)

                                                                                                                         aCATGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATaa             <  2:60701/140‑1 (MQ=255)

                                                                                                                         aCATGGC‑GGTTG‑GGATGGCGTTTGTCTGA‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATaa             <  1:1031209/140‑1 (MQ=255)

                                                                                                                            tGGC‑GGTTG‑GGGTGGGGTTTGTTTGG‑CTGGCGC‑GAC‑GG‑TTGCCC‑TGGTTAACTTTCTGGTCTGGGGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAAATTAACAATCAGCAACCGTTTTTGTGGCTTTACACTTATaaaat          >  2:452699/1‑137 (MQ=255)

                                                                                                                            tGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     >  2:553194/1‑129 (MQ=255)

                                                                                                                            tGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:553194/129‑1 (MQ=255)

                                                                                                                            tGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAggg          <  2:1118817/140‑1 (MQ=255)

                                                                                                                            tGGC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAggg          <  1:187450/140‑1 (MQ=255)

                                                                                                                             ggC‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGgt         <  2:1149203/140‑1 (MQ=255)

                                                                                                                               c‑GGTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGgtgt       >  2:680068/1‑140 (MQ=255)

                                                                                                                                 ggTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     <  1:714304/125‑1 (MQ=255)

                                                                                                                                 ggTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTac                     >  2:714304/1‑125 (MQ=255)

                                                                                                                                  gTTG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTa     <  2:712250/140‑1 (MQ=255)

                                                                                                                                    tG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTaag   <  1:26842/140‑1 (MQ=255)

                                                                                                                                    tG‑GGATGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTaag   <  1:1078062/140‑1 (MQ=255)

                                                                                                                                     g‑gggTGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAAATTAATGGTCTGGGGGGGGGGGTTTTCTCG‑TCA‑GGGAACCGCAAAATAAGCAATCAGCAACCGGTTTCGTGGCTTTACACTTATAAGGGGGTTaaaa  >  2:1078062/1‑138 (MQ=255)

                                                                                                                                     g‑gggTGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑CGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCTTGGCTTTTCACTTATAAGGGGGTTaaga  >  2:1401848/1‑140 (MQ=255)

CTAACCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGC‑GGTTG‑GGCTGGCGTTTGTCTGG‑CTGGCGC‑GAC‑GA‑TTGCCC‑TGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCG‑TCA‑GGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGA  >  NC_000913/2809270‑2809541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A