Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	27	74.7	2598802	99.1%	2575412	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,515	T→A	D182E (GAT→GAA)	ilvC →	ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,515	0	T	A	100.0%	358.6 / NA	101	D182E (GAT→GAA)	ilvC	ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (52/49); total (52/49)

Only 100 of 101 total aligned reads displayed.

GTCGAAGTGGGCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTGCGGTA  >  NC_000913/3958378‑3958651

gtcgAAGTGGGCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAAcc                                                                                                                                        >  2:467866/1‑140 (MQ=255)

gtcgAAGTGGGCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAAcc                                                                                                                                        >  1:1246472/1‑140 (MQ=255)

  cgAAGTGGGCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGa                                                                                                                                      <  1:130743/140‑1 (MQ=255)

        gggCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCg                                                                                                                                <  2:49623/140‑1 (MQ=255)

               cAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCAt                                                                                                                         >  2:96833/1‑140 (MQ=255)

               cAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCAt                                                                                                                         >  2:1287405/1‑140 (MQ=255)

                    ccGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    >  2:857715/1‑140 (MQ=255)

                    ccGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    <  1:271429/140‑1 (MQ=255)

                    ccGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    <  1:863300/140‑1 (MQ=255)

                        aaaGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGc                                                                                                                <  1:909882/140‑1 (MQ=255)

                         aaGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGcc                                                                                                               <  1:513217/140‑1 (MQ=255)

                         aaGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGcc                                                                                                               <  1:740543/140‑1 (MQ=255)

                            atatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCaaa                                                                                                            >  1:1164627/1‑140 (MQ=255)

                              atCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCGGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    <  1:768056/130‑1 (MQ=255)

                              atCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCGGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    >  2:768056/1‑130 (MQ=255)

                              atCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGc                                                                                                          >  1:484896/1‑140 (MQ=255)

                               tCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGcc                                                                                                         >  1:60420/1‑140 (MQ=255)

                                 aCCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTg                                                                                                       >  1:477812/1‑140 (MQ=255)

                                   cGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTggg                                                                                                     <  2:60420/140‑1 (MQ=255)

                                   cGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTggg                                                                                                     >  2:572304/1‑140 (MQ=255)

                                      aGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    >  2:1097207/1‑122 (MQ=255)

                                      aGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGa                                                                                                                    <  1:1097207/122‑1 (MQ=255)

                                          aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGTGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTgg                                                                                                      <  2:593147/132‑1 (MQ=255)

                                          aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGTGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTgg                                                                                                      >  1:593147/1‑132 (MQ=255)

                                          aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                              <  1:467866/140‑1 (MQ=255)

                                          aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                              >  1:732609/1‑140 (MQ=255)

                                            ggTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTggg                                                                                                     <  2:1223021/131‑1 (MQ=255)

                                            ggTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTggg                                                                                                     >  1:1223021/1‑131 (MQ=255)

                                            ggTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            <  1:572304/140‑1 (MQ=255)

                                            ggTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            <  1:712756/140‑1 (MQ=255)

                                             gTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAAc                                                                                           >  2:1162638/1‑140 (MQ=255)

                                             gTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAAc                                                                                           >  2:630029/1‑140 (MQ=255)

                                               tGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCg                                                                                         >  1:242091/1‑140 (MQ=255)

                                               tGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCg                                                                                         >  1:230717/1‑140 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            >  2:1021543/1‑135 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            >  1:613887/1‑135 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            <  1:671128/135‑1 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            <  1:1021543/135‑1 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            <  2:613887/135‑1 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCaa                                                                                            >  2:671128/1‑135 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                       >  2:577482/1‑140 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                       >  1:1052704/1‑140 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                       >  2:794112/1‑140 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                       >  2:437143/1‑140 (MQ=255)

                                                 cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                       >  2:293306/1‑140 (MQ=255)

                                                  gcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggtg                                                                                      >  2:265427/1‑140 (MQ=255)

                                                      aaaTGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCa                                                                                  <  2:1052704/140‑1 (MQ=255)

                                                      aaaTGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCa                                                                                  <  1:437143/140‑1 (MQ=255)

                                                         tGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCg                                                                               <  2:1246472/140‑1 (MQ=255)

                                                            ccAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGc                                                                            <  1:630029/140‑1 (MQ=255)

                                                              aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                          <  1:98321/140‑1 (MQ=255)

                                                               ggCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCggg                                                                         <  1:1162638/140‑1 (MQ=255)

                                                                gCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgggg                                                                        >  1:675994/1‑139 (MQ=255)

                                                                     gAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCt                                                                   >  1:174158/1‑140 (MQ=255)

                                                                     gAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCt                                                                   >  2:684503/1‑140 (MQ=255)

                                                                         tGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGaa                                                               <  1:857715/140‑1 (MQ=255)

                                                                          gCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAAt                                                              >  2:369128/1‑140 (MQ=255)

                                                                           cGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCggg                                                                         >  2:139221/1‑128 (MQ=255)

                                                                           cGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCggg                                                                         <  1:139221/128‑1 (MQ=255)

                                                                           cGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGaa                                                               >  1:223641/1‑138 (MQ=255)

                                                                           cGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGaa                                                               <  2:223641/138‑1 (MQ=255)

                                                                               aaGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTcc                                                         <  2:174158/140‑1 (MQ=255)

                                                                                        aaCGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCg                                                <  1:293306/140‑1 (MQ=255)

                                                                                        aaCGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCg                                                <  1:577482/140‑1 (MQ=255)

                                                                                         acatGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTc                                                          >  2:719071/4‑129 (MQ=255)

                                                                                         acatGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTc                                                          <  1:719071/126‑1 (MQ=255)

                                                                                            tGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAg                                            >  1:955081/1‑140 (MQ=255)

                                                                                              ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGt                                           >  2:844551/1‑139 (MQ=255)

                                                                                              ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGt                                           <  1:844551/139‑1 (MQ=255)

                                                                                              ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTg                                          >  2:910356/1‑140 (MQ=255)

                                                                                              ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTg                                          >  1:11107/1‑140 (MQ=255)

                                                                                              ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTg                                          >  1:660149/1‑140 (MQ=255)

                                                                                                  cGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAg                                            <  2:182718/134‑1 (MQ=255)

                                                                                                  cGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAg                                            >  1:182718/1‑134 (MQ=255)

                                                                                                  cGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAAt                                      >  2:920189/1‑140 (MQ=255)

                                                                                                  cGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAAt                                      >  1:1101565/1‑140 (MQ=255)

                                                                                                  cGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAAt                                      <  2:477812/140‑1 (MQ=255)

                                                                                                     cGTACCGACGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTg                                   >  1:241709/1‑140 (MQ=255)

                                                                                                             cGCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATg                           <  2:1088341/140‑1 (MQ=255)

                                                                                                              gCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAAt                                      <  1:1026850/128‑1 (MQ=255)

                                                                                                              gCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAAt                                      >  2:1026850/1‑128 (MQ=255)

                                                                                                              gCTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATgg                          <  1:265427/140‑1 (MQ=255)

                                                                                                               cTGATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATggg                         >  2:814417/1‑140 (MQ=255)

                                                                                                                 gATTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCg                       >  1:1030499/1‑140 (MQ=255)

                                                                                                                  aTTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                      <  1:729900/140‑1 (MQ=255)

                                                                                                                  aTTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                      <  1:814417/140‑1 (MQ=255)

                                                                                                                  aTTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                      <  2:11107/140‑1 (MQ=255)

                                                                                                                  aTTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                      <  2:1101565/140‑1 (MQ=255)

                                                                                                                  aTTGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                      <  2:230717/140‑1 (MQ=255)

                                                                                                                    tGCCGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGc                    >  2:1272799/1‑140 (MQ=255)

                                                                                                                      ccGTTCACCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCaa                  >  1:796426/1‑140 (MQ=255)

                                                                                                                            aCCCGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCTAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATc            <  1:634772/140‑1 (MQ=255)

                                                                                                                              ccGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCt          >  2:152637/1‑140 (MQ=255)

                                                                                                                              ccGGAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCt          <  1:238663/140‑1 (MQ=255)

                                                                                                                                 gAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg       <  1:1109560/140‑1 (MQ=255)

                                                                                                                                 gAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg       <  2:242091/140‑1 (MQ=255)

                                                                                                                                 gAAAACGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg       >  1:692291/1‑140 (MQ=255)

                                                                                                                                     aCGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTGCGGt   <  1:1249288/140‑1 (MQ=255)

                                                                                                                                     aCGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTGCGGt   <  1:708353/140‑1 (MQ=255)

                                                                                                                                      cGAACCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTGCGGTa  <  2:796426/140‑1 (MQ=255)

GTCGAAGTGGGCGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTGCGGTA  >  NC_000913/3958378‑3958651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A