Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	27	74.7	2598802	99.1%	2575412	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,002	C→T	intergenic (+2/‑74)	yigL → / → bioP	phosphosugar phosphatase YigL/biotin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,002	0	C	T	100.0%	165.3 / NA	47	intergenic (+2/‑74)	yigL/bioP	phosphosugar phosphatase YigL/biotin transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/32); total (15/32)

TCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAC  >  NC_000913/4010866‑4011138

tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGtt                                                                                                                                       >  1:51037/1‑140 (MQ=255)

     ggCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTa                                                                                                                                  >  2:363437/1‑140 (MQ=255)

           gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            <  2:117707/140‑1 (MQ=255)

            aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  1:916375/140‑1 (MQ=255)

            aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  2:687961/140‑1 (MQ=255)

            aaaGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTc                                                                                                                           <  2:1186752/140‑1 (MQ=255)

              aGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAg                                                                                                                         <  1:1223502/140‑1 (MQ=255)

                    cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            >  2:609598/1‑131 (MQ=255)

                    cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGt                                                                                                                            <  1:609598/131‑1 (MQ=255)

                         tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTg                                                                                                              >  1:820324/1‑140 (MQ=255)

                                tGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTc                                                                                                       >  1:975762/1‑140 (MQ=255)

                                 gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGt                                                                                                                     <  2:77825/125‑1 (MQ=255)

                                 gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGt                                                                                                                     >  1:77825/1‑125 (MQ=255)

                                 gcgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCg                                                                                                      <  1:1018712/140‑1 (MQ=255)

                                  cgcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGc                                                                                                     <  2:33178/140‑1 (MQ=255)

                                   gcACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCt                                                                                                    <  2:551316/140‑1 (MQ=255)

                                    cACCAGCGTCTGAAAGACCTTCATCCCTAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:25872/140‑1 (MQ=255)

                                    cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:390222/140‑1 (MQ=255)

                                    cACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  2:820324/140‑1 (MQ=255)

                                          cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   <  1:795742/134‑1 (MQ=255)

                                          cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTa                                                                                                   >  2:795742/1‑134 (MQ=255)

                                           gTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGa                                                                                            <  2:1013448/140‑1 (MQ=255)

                                            tCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGAt                                                                                           <  1:606955/140‑1 (MQ=255)

                                                   gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGtt                                                                                    <  1:363437/140‑1 (MQ=255)

                                                    aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTa                                                                                   >  2:258853/1‑140 (MQ=255)

                                                     ccTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTaa                                                                                  >  2:1227985/1‑140 (MQ=255)

                                                          aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCaaa                                                                             <  2:975762/140‑1 (MQ=255)

                                                              cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGt                                                                         >  2:1013268/1‑140 (MQ=255)

                                                                       aGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATtg                                                                <  1:1227985/140‑1 (MQ=255)

                                                                       aGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTCTCCATtg                                                                <  1:1013268/140‑1 (MQ=255)

                                                                          gATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTgg                                                             <  1:757649/140‑1 (MQ=255)

                                                                           aTTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgc                                                            >  2:301383/1‑140 (MQ=255)

                                                                            ttGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATAATTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcg                                                           <  1:254106/140‑1 (MQ=255)

                                                                             tGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcgc                                                          <  2:1046472/140‑1 (MQ=255)

                                                                             tGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGgcgc                                                          <  1:301383/140‑1 (MQ=255)

                                                                                     aTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTa                                                        >  2:394592/1‑134 (MQ=255)

                                                                                     aTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTa                                                        <  1:394592/134‑1 (MQ=255)

                                                                                      tGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTatca                                                 >  2:905206/1‑140 (MQ=255)

                                                                                          gacgacGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATcac                                             <  1:572868/140‑1 (MQ=255)

                                                                                            cgacgCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATcacca                                           <  2:51037/140‑1 (MQ=255)

                                                                                              acgCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACg                                         <  1:714521/140‑1 (MQ=255)

                                                                                               cgcgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGa                                        <  1:905206/140‑1 (MQ=255)

                                                                                                 cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      >  2:1846/1‑140 (MQ=255)

                                                                                                 cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      <  1:811241/140‑1 (MQ=255)

                                                                                                 cgGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGAtt                                      <  1:1182147/140‑1 (MQ=255)

                                                                                                                             tttATCGTAATTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATg          <  1:399544/140‑1 (MQ=255)

                                                                                                                                     aaTTGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAc  >  1:1208979/1‑140 (MQ=255)

TCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACTTCGCTACAATGGATACCCGTTAATCAAAGAGTTTTCCATTGTGGCGCTACTTATCATCACCACGATTCTGTGGGCCTTCTCCTTTAGCTTTTATGGCGAGTAC  >  NC_000913/4010866‑4011138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A