Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	57	32.8	3585168	30.6%	1097061	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,037,291	T→C	noncoding (32/76 nt)	alaT →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,037,291	0	T	C	100.0%	69.8 / NA	20	noncoding (32/76 nt)	alaT	tRNA‑Ala
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/8); total (12/8)

GGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCT  >  NC_000913/4037155‑4037338

gcctttagctcagctggttagagcacacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTg                                              <  2:202297‑M2/35‑1 (MQ=255)

   tttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGcaa                                           >  1:1192624‑M2/103‑139 (MQ=255)

    ttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTgcaag                                          >  1:1001111‑M2/102‑138 (MQ=255)

                    gagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGtt                          <  1:193817‑M2/55‑1 (MQ=255)

                       cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                       <  1:241233‑M2/58‑1 (MQ=255)

                        gcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAt                      <  2:240913‑M2/59‑1 (MQ=255)

                          acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                    >  2:1179866‑M2/80‑140 (MQ=255)

                          acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                    >  1:619772‑M2/80‑140 (MQ=255)

                             cctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGc                 <  2:414813‑M2/64‑1 (MQ=255)

                              ctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCgct                <  2:834224‑M2/65‑2 (MQ=255)

                                  taagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc            <  2:1001111‑M2/69‑6 (MQ=255)

                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:553503‑M2/68‑140 (MQ=255)

                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:626168‑M2/68‑140 (MQ=255)

                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:777186‑M2/68‑140 (MQ=255)

                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt      >  1:998237‑M2/66‑138 (MQ=255)

                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt      >  1:1195724‑M2/66‑138 (MQ=255)

                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt   >  1:797699‑M2/63‑135 (MQ=255)

                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt   >  2:984878‑M2/63‑135 (MQ=255)

                                            gtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCttggctccatttgtt  <  1:566582‑M2/79‑7 (MQ=255)

                                            gtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCttggctccatttgtt  >  2:566582‑M2/62‑134 (MQ=255)

GGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCT  >  NC_000913/4037155‑4037338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A