Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F60 I1 R1 57 32.8 3585168 30.6% 1097061 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,037,324 A→T noncoding (65/76 nt) alaT → tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,3240AT100.0% 18.8 / NA 7noncoding (65/76 nt)alaTtRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/0);  total (7/0)

GGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCT  >  NC_000913/4037193‑4037338
                                                                                                                                   |              
ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:553503‑M2/68‑140 (MQ=255)
ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:626168‑M2/68‑140 (MQ=255)
ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca        >  2:777186‑M2/68‑140 (MQ=255)
  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt      >  1:1195724‑M2/66‑138 (MQ=255)
  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt      >  1:998237‑M2/66‑138 (MQ=255)
     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt   >  1:797699‑M2/63‑135 (MQ=255)
     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt   >  2:984878‑M2/63‑135 (MQ=255)
      gtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCttggctccatttgtt  <  1:566582‑M2/79‑7 (MQ=255)
      gtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCttggctccatttgtt  >  2:566582‑M2/62‑134 (MQ=255)
                                                                                                                                   |              
GGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCT  >  NC_000913/4037193‑4037338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A