Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A35 F60 I1 R1	20	97.3	5307866	61.2%	3248413	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,296,381	+GC	intergenic (+587/+55)	gltP → / ← yjcO	glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,380	1	.	C	98.6%	256.9 / ‑3.3	69	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (30/38); total (31/38)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
*	NC_000913	4,296,380	2	.	G	98.6%	255.2 / ‑3.3	69	intergenic (+586/+56)	gltP/yjcO	glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (30/38); total (31/38)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGT  >  NC_000913/4296303‑4296509

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aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAAc                                                                       >  2:2190774/1‑140 (MQ=0)

          aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                             >  2:2437434/1‑140 (MQ=255)

               gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                        >  2:1936989/1‑140 (MQ=255)

               gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                        >  1:1179798/1‑140 (MQ=255)

               gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                        >  2:1402157/1‑140 (MQ=255)

               gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                        >  1:2555510/1‑140 (MQ=255)

                ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                       >  2:1474171/1‑140 (MQ=255)

                 cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                                                      >  1:1121559/1‑140 (MQ=255)

                 cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                                                      >  2:1231372/1‑140 (MQ=255)

                  tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                     <  1:1051071/140‑1 (MQ=255)

                  tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                     <  2:1368168/140‑1 (MQ=255)

                  tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa                                                     >  2:1251092/1‑140 (MQ=255)

                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                    <  1:1024104/140‑1 (MQ=255)

                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                    <  2:1923351/140‑1 (MQ=255)

                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                    <  2:2138561/140‑1 (MQ=255)

                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATACGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                    >  2:1748405/1‑140 (MQ=255)

                    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                                   >  1:1845618/1‑140 (MQ=255)

                     gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CGCCGCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                    >  1:1559031/1‑140 (MQ=14)

                        aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                               >  1:2571492/1‑140 (MQ=255)

                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                              >  1:2107793/1‑140 (MQ=255)

                          aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                             >  2:1341003/1‑140 (MQ=255)

                          aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                             <  1:2124064/140‑1 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac                                          >  2:2154883/1‑140 (MQ=17)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  2:2620265/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1367583/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         <  1:1503416/140‑1 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1143945/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         <  1:2412523/140‑1 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1111827/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1989336/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1831941/1‑140 (MQ=255)

                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                         >  1:1666309/1‑140 (MQ=255)

                                cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                       <  1:2242198/140‑1 (MQ=255)

                                cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                       <  1:1884574/140‑1 (MQ=255)

                                   tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                                    <  1:2243577/140‑1 (MQ=255)

                                    aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt                                   >  2:2500089/1‑140 (MQ=255)

                                      ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                                 >  2:2185006/1‑140 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:2059780/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:2305808/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:2461239/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               >  2:1904890/1‑140 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:1831941/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:1457401/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  1:1615134/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  2:1456091/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  1:1874215/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  1:2150953/140‑1 (MQ=255)

                                        tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                               <  1:2382608/140‑1 (MQ=255)

                                          aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                             >  1:2373762/1‑140 (MQ=255)

                                           tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                            >  1:2218670/1‑140 (MQ=255)

                                             tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                          <  1:2102242/140‑1 (MQ=255)

                                                  tGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                     <  2:1801188/140‑1 (MQ=255)

                                                   gATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                    <  1:2141457/140‑1 (MQ=255)

                                                    aTTTTGTAGGTCTGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                   >  2:1575339/1‑140 (MQ=255)

                                                    aTTTTGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                   >  2:1945108/1‑140 (MQ=255)

                                                       ttGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACGGCt                <  2:2598847/140‑1 (MQ=255)

                                                       ttGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                <  1:1572288/140‑1 (MQ=255)

                                                       ttGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                <  1:1765632/140‑1 (MQ=255)

                                                       ttGTAGGTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                <  1:1959276/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  1:2437434/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  1:2549843/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  2:1367583/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  2:1551775/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  2:2555510/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  1:1341003/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  1:1474171/140‑1 (MQ=255)

                                                            ggTCGGATAAGGCGTCTATGC‑‑‑‑TGCATCGGACATCAATGCCTGATGCGACGCTTGGCGC‑GTTTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt           <  2:2459917/140‑1 (MQ=38)

                                                              tCGGATAATGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCTTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGCCCGGTATCAATCCCTTCCATACAGCTCAGGTTc         <  1:1553731/140‑1 (MQ=25)

                                                                 gATAAGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc      <  2:1932779/140‑1 (MQ=255)

                                                                     aGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGt  <  2:1121559/140‑1 (MQ=255)

                                                                     aGGCGTTTACGC‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGt  <  2:1529055/140‑1 (MQ=255)

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AACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGC‑GTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGT  >  NC_000913/4296303‑4296509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A