Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A33 F60 I1 R1	23	100.4	3424510	99.4%	3403962	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,274,902	C→T	G47D (GGC→GAC)	yjcB ←	uncharacterized protein YjcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,274,902	0	C	T	98.5%	461.5 / ‑1.8	135	G47D (GGC→GAC)	yjcB	uncharacterized protein YjcB
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (70/63); total (71/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 141 total aligned reads displayed.

CAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGT  >  NC_000913/4274766‑4275035

cAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCa                                                                                                                                    <  2:1198853/140‑1 (MQ=255)

   aaTCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGAc                                                                                                                                 >  2:1213552/1‑140 (MQ=255)

    aTCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTG‑GATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCg                                                                                                                               <  1:1185783/140‑1 (MQ=255)

     tCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCg                                                                                                                               <  2:1536361/140‑1 (MQ=255)

        ttGGCCATTGTGGCGGTGTCTGATTCATCTCTTTTATCATCACCCACTCCATGTTGTGCCAGATAGCGGGGATGTCCCCCATTGTGGTTCCAATCAACCCCATTTCCAAACAACAGGCGGAAACAAAGGCCAGACCcctg                                                                                                                            >  2:1047687/1‑140 (MQ=255)

        ttGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCgctg                                                                                                                            >  2:1359073/1‑140 (MQ=255)

         tGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCgctgc                                                                                                                           <  1:61626/140‑1 (MQ=255)

             cATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGAc                                                                                                                                 <  2:1140507/130‑1 (MQ=255)

                tgtgGCGGCTGCTGGTTCTTCTCTTCTATCATCACCCACCCGCTGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCCAGCAACCCCACTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCAc                                                                                                                    >  2:734596/1‑140 (MQ=255)

                  tgGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCg                                                                                                                  <  2:1221594/140‑1 (MQ=255)

                     cGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCg                                                                                                                               <  1:102935/124‑1 (MQ=255)

                       gtgtCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGc                                                                                                             <  1:733255/140‑1 (MQ=255)

                       gtgtCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGc                                                                                                             <  2:950265/140‑1 (MQ=255)

                        tgtCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGcc                                                                                                            <  1:1639150/140‑1 (MQ=255)

                           cTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGa                                                                                                         <  2:466851/140‑1 (MQ=255)

                              aTTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACAt                                                                                                      >  2:242555/1‑140 (MQ=255)

                                  aTCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATc                                                                                                  >  1:1182355/1‑140 (MQ=255)

                                    ctctTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAg                                                                                                >  1:561075/1‑140 (MQ=255)

                                        tctATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCAGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcg                                                                                            >  1:1243149/1‑140 (MQ=255)

                                         ctATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGa                                                                                                         >  2:1332151/1‑126 (MQ=255)

                                         ctATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGa                                                                                                         <  1:1332151/126‑1 (MQ=255)

                                         ctATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACcga                                                                                           >  2:234138/1‑140 (MQ=255)

                                              atcaCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaa                                                                                      >  1:934318/1‑140 (MQ=255)

                                               tcaCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaaa                                                                                     <  2:514952/140‑1 (MQ=255)

                                               tcaCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaaa                                                                                     <  1:234138/140‑1 (MQ=255)

                                                 aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACg                                                                                   >  1:1700993/1‑140 (MQ=255)

                                                 aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACg                                                                                   >  1:694921/1‑140 (MQ=255)

                                                 aCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACg                                                                                   >  1:369151/1‑140 (MQ=255)

                                                  ccGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACgg                                                                                  <  2:694921/140‑1 (MQ=255)

                                                  ccGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACgg                                                                                  <  1:1484484/140‑1 (MQ=255)

                                                   cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGa                                                                                               >  1:755924/1‑126 (MQ=255)

                                                   cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGa                                                                                               <  2:755924/126‑1 (MQ=255)

                                                   cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaaa                                                                                     >  1:652377/1‑136 (MQ=255)

                                                   cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGa                                                                                 >  2:836689/1‑140 (MQ=255)

                                                   cGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGa                                                                                 >  1:86951/1‑140 (MQ=255)

                                                    gACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGAt                                                                                >  2:5682/1‑140 (MQ=255)

                                                    gACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGAt                                                                                >  1:981674/1‑140 (MQ=255)

                                                      cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGt                                                                              >  1:1020887/1‑140 (MQ=255)

                                                      cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGt                                                                              >  1:1375545/1‑140 (MQ=255)

                                                      cTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGt                                                                              >  1:771905/1‑140 (MQ=255)

                                                        cGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaaa                                                                                     <  1:1322343/131‑1 (MQ=255)

                                                        cGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGaaaa                                                                                     >  2:1322343/1‑131 (MQ=255)

                                                         gATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGa                                                                           <  1:1047687/140‑1 (MQ=255)

                                                         gATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGa                                                                           <  1:1359073/140‑1 (MQ=255)

                                                              tgtgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGtgtg                                                                      <  2:1375545/140‑1 (MQ=255)

                                                              tgtgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGtgtg                                                                      <  2:1450377/140‑1 (MQ=255)

                                                               gtgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGc                                                                     <  2:1700993/140‑1 (MQ=255)

                                                                tgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCt                                                                    >  2:213230/1‑140 (MQ=255)

                                                                tgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCt                                                                    >  1:1693211/1‑140 (MQ=255)

                                                                tgCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGGGTGCt                                                                    >  2:472925/1‑140 (MQ=255)

                                                                   cAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGc                                                                 <  1:93250/140‑1 (MQ=255)

                                                                     gATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCa                                                               >  1:866407/1‑140 (MQ=255)

                                                                     gATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCa                                                               >  1:1679338/1‑140 (MQ=255)

                                                                       tAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                             >  1:892807/1‑140 (MQ=255)

                                                                          cGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAAc                                                          >  2:1503405/1‑140 (MQ=255)

                                                                          cGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAAc                                                          >  2:786313/1‑140 (MQ=255)

                                                                                tGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCAtt                                                    >  2:280619/1‑140 (MQ=255)

                                                                                tGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAA‑ATCATTa                                                   >  1:730831/1‑140 (MQ=255)

                                                                                 gTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                   <  1:1134088/140‑1 (MQ=255)

                                                                                  tCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTAc                                                  <  2:1044835/140‑1 (MQ=255)

                                                                                  tCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTAc                                                  >  1:170458/1‑140 (MQ=255)

                                                                                    cGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACgg                                                <  1:1213552/140‑1 (MQ=255)

                                                                                    cGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACgg                                                <  1:280619/140‑1 (MQ=255)

                                                                                      ccATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCa                                              <  2:208016/140‑1 (MQ=255)

                                                                                      ccATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCa                                              <  1:786313/140‑1 (MQ=255)

                                                                                       cATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCAc                                             >  2:1623571/1‑140 (MQ=255)

                                                                                       cATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCAc                                             >  1:201562/1‑140 (MQ=255)

                                                                                        aTTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACt                                            <  1:1430686/140‑1 (MQ=255)

                                                                                        aTTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACt                                            <  2:1020887/140‑1 (MQ=255)

                                                                                        aTTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACt                                            <  2:369151/140‑1 (MQ=255)

                                                                                            tgATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTaaga                                        >  1:312692/1‑140 (MQ=255)

                                                                                            tgATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTaaga                                        >  1:1080493/1‑140 (MQ=255)

                                                                                                 ccAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTg                                   >  2:636639/1‑140 (MQ=255)

                                                                                                   aaGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGcc                                 >  2:622234/1‑140 (MQ=255)

                                                                                                    aGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCa                                >  1:404789/1‑140 (MQ=255)

                                                                                                    aGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCa                                >  2:559184/1‑140 (MQ=255)

                                                                                                    aGCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCa                                >  2:10158/1‑140 (MQ=255)

                                                                                                     gCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                               <  2:312692/140‑1 (MQ=255)

                                                                                                     gCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                               >  1:304563/1‑140 (MQ=255)

                                                                                                     gCAACCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                               <  1:242555/140‑1 (MQ=255)

                                                                                                       aaCCCCATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCg                             <  1:559184/140‑1 (MQ=255)

                                                                                                         ccccATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGc                                  <  1:386461/133‑1 (MQ=255)

                                                                                                         ccccATTGCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGc                                  >  2:386461/1‑133 (MQ=255)

                                                                                                                gCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAAc                    >  2:182849/1‑140 (MQ=255)

                                                                                                                gCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAAc                    <  2:401638/140‑1 (MQ=255)

                                                                                                                gCGAACCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAAc                    <  1:10158/140‑1 (MQ=255)

                                                                                                                   aaCCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCAc                 >  1:560762/1‑140 (MQ=255)

                                                                                                                   aaCCAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCAc                 >  1:1101835/1‑140 (MQ=255)

                                                                                                                     ccAACAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGc               <  1:1503405/140‑1 (MQ=255)

                                                                                                                       aaCAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGATCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCa             <  2:170458/140‑1 (MQ=255)

                                                                                                                       aaCAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCa             <  2:935690/140‑1 (MQ=255)

                                                                                                                        aCAGGCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAg            >  2:1398713/1‑140 (MQ=255)

                                                                                                                           ggCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtgg         <  1:483362/140‑1 (MQ=255)

                                                                                                                           ggCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtgg         <  2:1442003/140‑1 (MQ=255)

                                                                                                                           ggCGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtgg         >  2:1152669/1‑140 (MQ=255)

                                                                                                                             cGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtggtg       <  1:1122825/140‑1 (MQ=255)

                                                                                                                             cGGAAACAACGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtggtg       >  1:1222039/1‑140 (MQ=255)

                                                                                                                                 aacaacGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAggg   <  1:1647916/140‑1 (MQ=255)

                                                                                                                                  acaacGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGt  <  1:366260/140‑1 (MQ=255)

                                                                                                                                  acaacGTCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGt  <  1:58874/140‑1 (MQ=255)

CAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACAGGCGGAAACAACGCCCAGACCGCTGCTGATCACCGCAAGCCCGACATAATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGT  >  NC_000913/4274766‑4275035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A