Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F60 I1 R1	18	61.6	2092650	99.2%	2075908	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,265,234	T→G	E265D (GAA→GAC)	tdcB ←	catabolic threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,265,234	0	T	G	99.0%	356.9 / NA	95	E265D (GAA→GAC)	tdcB	catabolic threonine dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (60/34); total (60/35)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAATACTGACGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGT  >  NC_000913/3265099‑3265367

aataatACTGACGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGt                                                                                                                                   <  1:1030293/140‑1 (MQ=255)

     taCTGACGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCg                                                                                                                              <  2:667677/140‑1 (MQ=255)

         gACGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCTAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGa                                                                                                                          >  2:191978/1‑140 (MQ=255)

          aCGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGAc                                                                                                                         >  2:981345/1‑140 (MQ=255)

          aCGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGAc                                                                                                                         >  1:912195/1‑140 (MQ=255)

          aCGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGAc                                                                                                                         >  2:663785/1‑140 (MQ=255)

          aCGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGAc                                                                                                                         <  1:574890/140‑1 (MQ=255)

          aCGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGACGTCGTCTTCGCTGAc                                                                                                                         >  1:421993/1‑140 (MQ=255)

           cGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGAcc                                                                                                                        >  2:874556/1‑140 (MQ=255)

               ttttCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGt                                                                                                                                   >  1:703864/1‑125 (MQ=255)

               ttttCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGt                                                                                                                                   <  2:703864/125‑1 (MQ=255)

               ttttCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCa                                                                                                                    <  1:663785/140‑1 (MQ=255)

                 ttCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACg                                                                                                                  <  1:981345/140‑1 (MQ=255)

                      ttttGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTc                                                                                                             >  1:885214/1‑140 (MQ=255)

                                atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAAt                                                                                                   >  1:1007485/1‑140 (MQ=255)

                                atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAAt                                                                                                   >  1:813593/1‑140 (MQ=255)

                                     tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCTGCACGATGTGATCGACTAATTCacg                                                                                              >  1:439317/1‑140 (MQ=255)

                                     tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCacg                                                                                              >  1:29644/1‑140 (MQ=255)

                                     tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCacg                                                                                              >  1:243526/1‑140 (MQ=255)

                                     tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGACTTCGCTGACCAGCACGATGTTATCGACTAATTCacg                                                                                              >  1:350796/1‑140 (MQ=255)

                                     tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTAGTCATAGCTGACCAGCTCGATATCATCGACTAATTCacg                                                                                              >  2:471603/1‑140 (MQ=255)

                                      cTAATTTACCGCTTAATAATGCCGCACATGCCAGAGCGCCTGCGCCTTAGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCacga                                                                                             <  2:288198/140‑1 (MQ=255)

                                         aTTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTATGGCAATCATACTGTTTCTGATGTCGTCTTTGCTGACCCGCAGGAAGTCATCCACTAATTCacgaacg                                                                                          >  1:366408/1‑140 (MQ=255)

                                          tttACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCacgaacga                                                                                         >  1:767689/1‑140 (MQ=255)

                                          tttACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCacgaacga                                                                                         >  2:203652/1‑140 (MQ=255)

                                               cGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCACTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCg                                                                                    >  2:671594/1‑140 (MQ=255)

                                                 cTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTa                                                                                  >  1:980369/1‑140 (MQ=255)

                                                   taataaTGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAg                                                                                <  2:818137/140‑1 (MQ=255)

                                                         tGCAGCACATGCCAGAGCGCCTACGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATATCATCGATTAAGTCACGAACGATTTCGTAAGTTAAAt                                                                          >  1:267175/1‑140 (MQ=255)

                                                           cagcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTa                                                                        >  2:796988/1‑140 (MQ=255)

                                                           cagcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTa                                                                        >  1:816517/1‑140 (MQ=255)

                                                            agcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAc                                                                       >  2:714400/1‑140 (MQ=255)

                                                             gcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAcc                                                                      <  2:816517/140‑1 (MQ=255)

                                                             gcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCACTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAcc                                                                      <  1:671594/140‑1 (MQ=255)

                                                             gcaCATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTAGTGCGCTGACTTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAcc                                                                      <  2:420908/140‑1 (MQ=255)

                                                             gcaCATGCCAGAGCGCCCGCGCGTTCGGTGAGGACATTATTGCGCTGATTTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAcc                                                                      <  2:439317/140‑1 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  1:690560/1‑140 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  1:664683/1‑140 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  1:629957/1‑140 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  1:166791/1‑140 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     <  2:980369/140‑1 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  2:766784/1‑140 (MQ=255)

                                                              cacaTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTAccc                                                                     >  2:996871/1‑140 (MQ=255)

                                                                caTGCCAGTGCGCCTGATCCTTCGGTGACGACTTTCTTGTGCTGAATTTAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCcgg                                                                   <  2:500208/140‑1 (MQ=255)

                                                                caTGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCcgg                                                                   >  1:233882/1‑140 (MQ=255)

                                                                 aTGCCAGAGCGCCTGCGACTTCCGTGACGACGTTAATGCTCTGAATTAGGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCcggg                                                                  <  2:366408/140‑1 (MQ=255)

                                                                   gCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTaa                                                                                 >  2:158387/1‑123 (MQ=255)

                                                                   gCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTaa                                                                                 <  1:158387/123‑1 (MQ=255)

                                                                   gCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCcgggcg                                                                >  1:963307/1‑140 (MQ=255)

                                                                   gCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCcgggcg                                                                >  2:786604/1‑140 (MQ=255)

                                                                   gCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGAGGTCATCGACTAATTAACTAACGATTACGTAATTTAAATTACCcgggcg                                                                >  2:509074/1‑140 (MQ=255)

                                                                      agagCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGga                                                             <  2:6617/140‑1 (MQ=255)

                                                                        agCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGgaga                                                           >  1:782608/1‑140 (MQ=255)

                                                                          cgcctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAAGTACCCGGGCGGGAGACa                                                         >  2:326197/1‑140 (MQ=255)

                                                                            cctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATc                                                       >  2:237567/1‑140 (MQ=255)

                                                                             ctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATca                                                      <  2:29644/140‑1 (MQ=255)

                                                                             ctgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATca                                                      >  2:578504/1‑140 (MQ=255)

                                                                              tgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATcac                                                     <  2:233882/140‑1 (MQ=255)

                                                                              tgcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATcac                                                     <  2:953262/140‑1 (MQ=255)

                                                                               gcgcctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATcaca                                                    >  2:987243/1‑140 (MQ=255)

                                                                                  cctTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAAcc                                                 <  1:584897/140‑1 (MQ=255)

                                                                                           acgacTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAg                                        <  2:885214/140‑1 (MQ=255)

                                                                                           acgacTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAg                                        <  1:578504/140‑1 (MQ=255)

                                                                                              acTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACGTCACAACCATCCGCCAGGGt                                     <  1:573744/140‑1 (MQ=255)

                                                                                              acTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGt                                     <  2:1007485/140‑1 (MQ=255)

                                                                                                  tATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCg                                 >  1:962939/1‑140 (MQ=255)

                                                                                                    ttGCGCTGAATTAAGGCCATCAAACTGTTTCTGATTTCGACTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGt                               <  2:350796/140‑1 (MQ=255)

                                                                                                       cgcTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGt                            >  1:644305/1‑140 (MQ=255)

                                                                                                        gcTGAATTAAGGCTATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGtt                           <  2:962939/140‑1 (MQ=255)

                                                                                                         cTGAATTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATTACAACCGTCCGCCAGGGTGCCGGTAGTTc                          >  1:289316/1‑140 (MQ=255)

                                                                                                         cTGAATTAAGGCAATAATACTGTTTTTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTc                          <  1:509074/140‑1 (MQ=255)

                                                                                                            aactAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGt                       <  2:629957/137‑1 (MQ=255)

                                                                                                            aaTTAAGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGt                       >  1:101659/1‑140 (MQ=255)

                                                                                                                 aGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGtt                           <  1:567897/131‑1 (MQ=255)

                                                                                                                 aGGCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGtt                           >  2:567897/1‑131 (MQ=255)

                                                                                                                   gCAATCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGt                <  1:786604/140‑1 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGTGACATCACAACCATCCTCCAGGGTGCCGGTAGTTCGGTGCGTGGTTAt             >  2:379414/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGCGACATCACAACCATCCGCCAGGGTGGCGGTAATTCGGTGCGTGGTTAt             >  2:479250/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTAt             >  1:180293/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTAt             >  2:872792/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGAGCCGGTAGTTCGGTGCGTGGTTAt             >  2:71690/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAAACATCCGCCAGGTTGCCGGTAGTTCGGTGCGTGGTTAt             >  1:452300/1‑140 (MQ=255)

                                                                                                                      aTCATACTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGCTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTAt             >  1:681614/1‑140 (MQ=255)

                                                                                                                      aTCATACGGTTTCTGATGTGGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTAt             >  2:604845/1‑140 (MQ=255)

                                                                                                                           aCTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTtctc        <  2:813593/140‑1 (MQ=255)

                                                                                                                           aCTGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTtctc        <  2:539195/140‑1 (MQ=255)

                                                                                                                             tGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCg      >  2:52950/1‑140 (MQ=255)

                                                                                                                             tGTTTCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCg      <  1:874556/140‑1 (MQ=255)

                                                                                                                              gTTTCTGATGTCGTCTTCGCTGTCCAGCACGATGTCAACGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTCCTCCgg     <  1:471603/140‑1 (MQ=255)

                                                                                                                               tttCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGa    >  2:872019/1‑140 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAg   <  1:581832/139‑1 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAg   >  2:581832/1‑139 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt  >  1:29606/1‑140 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt  >  1:162049/1‑140 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt  >  2:713734/1‑140 (MQ=255)

                                                                                                                                 tCTGATGTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTAGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGt  >  2:104717/1‑140 (MQ=255)

AATAATACTGACGGTTTTTCTGTTTTGAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCCTTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACGATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATTACCCGGGCGGGAGACATCACAACCATCCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTATTTCTCCGGAGT  >  NC_000913/3265099‑3265367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A