Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A34 F60 I1 R1 17 86.3 2937582 99.1% 2911143 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,342,729 A→G T273T (ACA→ACG)  melA → alpha‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,342,7290AG97.2% 264.5 / NA 73T273T (ACA→ACG) melAalpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (34/36);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCC  >  NC_000913/4342595‑4342862
                                                                                                                                      |                                                                                                                                     
gcTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTg                                                                                                                                  <  2:513109/140‑1 (MQ=255)
         gcagggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTaa                                                                                                                         >  1:732890/1‑140 (MQ=255)
           agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGc                                                                                                                       >  2:227848/1‑140 (MQ=255)
           agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGc                                                                                                                       <  2:1280847/140‑1 (MQ=255)
            gggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTTGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGcc                                                                                                                      <  1:890601/140‑1 (MQ=255)
             ggcaggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCa                                                                                                                     >  2:1094412/1‑140 (MQ=255)
             ggcaggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACAGAATCGTCAGAATATTTTGCTGAGTGCACGTCGTGGTTTGTTAAGCCa                                                                                                                     >  2:675549/1‑140 (MQ=255)
               caggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAgg                                                                                                                   <  1:1406169/140‑1 (MQ=255)
                   caCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGt                                                                                                               <  1:1098555/140‑1 (MQ=255)
                   caCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGt                                                                                                               <  1:310968/140‑1 (MQ=255)
                   caCCGAAGCCGAATATTCATGGCAATACTCGCTGCCACAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGt                                                                                                               <  1:647210/140‑1 (MQ=255)
                       gAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGc                                                                                                                       <  2:908040/128‑1 (MQ=255)
                       gAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGc                                                                                                                       >  1:908040/1‑128 (MQ=255)
                        aaGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGGTTATTAAGCCAGGTCGTGAGGa                                                                                                          >  1:533169/1‑140 (MQ=255)
                         aGCCGAATATTCATGTCAATACGCGATGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGAt                                                                                                         <  1:675549/140‑1 (MQ=255)
                             gAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttga                                                                                                     >  1:68165/1‑140 (MQ=255)
                              aaTATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGt                                                                                                               <  1:119841/129‑1 (MQ=255)
                              aaTATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGt                                                                                                               >  2:119841/1‑129 (MQ=255)
                              aaTATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttgat                                                                                                    >  2:1290198/1‑140 (MQ=255)
                               atatTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttgatt                                                                                                   <  1:1466735/140‑1 (MQ=255)
                               atatTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttgatt                                                                                                   <  2:732890/140‑1 (MQ=255)
                               atatTCATGGCAATACTCGCTGCCAGAATATAGTGAGCTACGAAATGTACAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttgatt                                                                                                   <  2:409158/140‑1 (MQ=255)
                                 atTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATttgattga                                                                                                 <  2:1342406/140‑1 (MQ=255)
                                  ttCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAg                                                                                                <  1:1094412/140‑1 (MQ=255)
                                    cATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCg                                                                                              <  1:1290198/140‑1 (MQ=255)
                                     aTGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGt                                                                                             <  2:1185730/140‑1 (MQ=255)
                                       ggCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTta                                                                                           >  2:255704/1‑140 (MQ=255)
                                        gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTtat                                                                                          >  1:273383/1‑140 (MQ=255)
                                            tACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCTTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAg                                                                                      >  2:1236250/1‑140 (MQ=255)
                                             aCTCGCTGCCAGAATATTGTGCCCTACGAAACGTTTAAATAGCTGGGCGATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGt                                                                                     <  2:533169/140‑1 (MQ=255)
                                             aCTCGCTGACAGAATAGAGTGCGCTACGAAGGGGTGAAACAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGt                                                                                     <  2:704520/140‑1 (MQ=255)
                                               tCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTAc                                                                                   <  1:1191079/140‑1 (MQ=255)
                                               tCGCTGCCAGAATATTGAGCGCTACGAAATGTTCACAAATCTGGGCTAATTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTAc                                                                                   <  2:415400/140‑1 (MQ=255)
                                                 gCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCGAGGTCGTGAGGCTTGGATTGAACGTTATAAAGTAccc                                                                                 >  1:704520/1‑139 (MQ=255)
                                                 gCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCg                                                                                 >  2:190143/1‑140 (MQ=255)
                                                  cTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGACAGGTCGGGAGGATTTCATTGAGCGTTATAAAGTACCGc                                                                                >  2:537121/1‑140 (MQ=255)
                                                   tGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTTAGGATTTGTTTGAACGTTATAAAGTACCGCt                                                                               >  2:461531/1‑140 (MQ=255)
                                                      cAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGa                                                                            >  2:1409292/1‑140 (MQ=255)
                                                           tatTGTGCGCTACGAAATGTTCCAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGt                                                                       <  1:590282/140‑1 (MQ=255)
                                                           tatTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGt                                                                       <  1:227848/140‑1 (MQ=255)
                                                                  cgcTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATGAAATACCGCAGGATGAGTACCCGaa                                                                >  1:708941/1‑140 (MQ=255)
                                                                   gcTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGaaa                                                               >  2:720872/1‑140 (MQ=255)
                                                                   gcTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGaaa                                                               >  2:260811/1‑140 (MQ=255)
                                                                     tACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGa                                                                         <  2:1228175/128‑1 (MQ=255)
                                                                     tACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGa                                                                         >  1:1228175/1‑128 (MQ=255)
                                                                     tACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACg                                                             >  1:1123403/1‑140 (MQ=255)
                                                                     tACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACg                                                             >  2:152634/1‑140 (MQ=255)
                                                                       cGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTAAAAAGTACCACTGGCTGAGTACCCGAAACGCt                                                           >  2:432079/1‑140 (MQ=255)
                                                                       cGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGC‑GAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTg                                                          >  1:1280316/1‑140 (MQ=255)
                                                                          aaTGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCg                                                        >  1:300499/1‑140 (MQ=255)
                                                                                 aaaaaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGGGCGTTATAGAGTTCCGATGGATGAGGACCCTAAACGCTGCGTCGagca                                                 >  2:404388/1‑140 (MQ=255)
                                                                                 aaaaaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGagca                                                 >  2:211093/1‑140 (MQ=255)
                                                                                         gggCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGGTGGATGAGTACCCGAATCGCTCCGTCGAGCAGCTGGCGa                                         >  2:454219/1‑140 (MQ=255)
                                                                                          ggCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTGGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGaa                                        <  1:1236250/140‑1 (MQ=255)
                                                                                                   gTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATaa                               <  2:1312762/140‑1 (MQ=255)
                                                                                                   gTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATaa                               <  1:140808/140‑1 (MQ=255)
                                                                                                   gTCACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATaa                               <  1:152634/140‑1 (MQ=255)
                                                                                                    tCACGGAATCGTCAGCACATTTTGCTGAGGACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATaaa                              <  1:614448/140‑1 (MQ=255)
                                                                                                     cACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACt                                      <  2:120209/131‑1 (MQ=255)
                                                                                                     cACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACt                                      >  1:120209/1‑131 (MQ=255)
                                                                                                     cACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAag                             >  2:916348/1‑140 (MQ=255)
                                                                                                     cACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAag                             <  1:281338/140‑1 (MQ=255)
                                                                                                     cACGGAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAag                             >  2:281338/1‑140 (MQ=255)
                                                                                                        ggAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGc                          <  2:1123403/140‑1 (MQ=255)
                                                                                                         gAATCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGTGTACCCGACACGATGCATCGAGCAGTTGGCCAACTGGCATAAAGAGcc                         >  2:614448/1‑139 (MQ=255)
                                                                                                          aaTCGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTg                        <  2:300499/140‑1 (MQ=255)
                                                                                                             cGTCAGAACATTTTGCTGAGTATACACCGCGGTTTATCAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTggag                     <  1:404388/140‑1 (MQ=255)
                                                                                                             cGTCAGAACATTTTGCTGAGTACACTCCGTGGTTTATGAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTggag                     <  1:432079/140‑1 (MQ=255)
                                                                                                             cGTCAGAACATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTggag                     <  2:68165/140‑1 (MQ=255)
                                                                                                             cGTCAGAACATTTTGATGAGTAAACGCCGAGGTTTATGAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTggag                     <  1:537121/140‑1 (MQ=255)
                                                                                                                cAGAACATTTTGC‑GAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGt                 <  2:1280316/140‑1 (MQ=255)
                                                                                                                    aCATTTTGCTGAGTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGtata              >  2:1048246/1‑140 (MQ=255)
                                                                                                                                gTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAGAGAGCTGGAGGAGGATAAAAAAGCCTccc  >  1:431874/1‑140 (MQ=255)
                                                                                                                                gTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTccc  >  1:124126/1‑140 (MQ=255)
                                                                                                                                gTACACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTccc  <  1:211093/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GCTTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCC  >  NC_000913/4342595‑4342862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A