Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F60 I1 R1	18	145.0	4913530	99.1%	4869308	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,273,801	T→C	D24G (GAC→GGC)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,273,801	0	T	C	99.4%	583.5 / NA	158	D24G (GAC→GGC)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (77/79); total (78/80)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

TTCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGTCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACAAAC  >  NC_000913/4273665‑4273933

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGTACCGGAAAGCCCGGTCAGGACAATGAGCTTGCcgc                                                                                                                                       >  2:739724/1‑140 (MQ=255)

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc                                                                                                                                       <  2:91789/140‑1 (MQ=255)

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc                                                                                                                                       <  2:151704/140‑1 (MQ=255)

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc                                                                                                                                       >  2:1580483/1‑140 (MQ=255)

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc                                                                                                                                       <  2:1997257/140‑1 (MQ=255)

ttCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTACCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc                                                                                                                                       <  2:2127081/140‑1 (MQ=255)

 tCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTACCGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc‑‑g                                                                                                                                    <  1:2262292/140‑1 (MQ=255)

 tCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc‑‑g                                                                                                                                    <  1:338600/140‑1 (MQ=255)

 tCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCcgc‑‑g                                                                                                                                    <  2:1779914/140‑1 (MQ=255)

     tCAGTGACAGAAACTACCGCGCATAGGCGGAAAGGGACTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCTGGTCACGACAATGAGCTTGCCGC‑‑ggg‑‑gg                                                                                                                              <  1:1186692/140‑1 (MQ=255)

        gTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATa                                                                                                                           <  1:2064830/140‑1 (MQ=255)

         tGACAGAAACTGCCGTGCGTAGGCGTAAAAGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATaa                                                                                                                          <  1:888690/140‑1 (MQ=255)

           aCAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCGGAACCCGAAAGCACGGTTACGACAAGGAGCGTGCCGC‑‑GGC‑‑GGATAACg                                                                                                                        >  2:1120034/1‑140 (MQ=255)

               aaaCTGCCCCGCGTAGCGGGAAGGGGATTCAACGTAACGTCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGt                                                                                                                    <  2:1181466/140‑1 (MQ=255)

                 aCTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑ggg‑‑g                                                                                                                               <  2:2381061/127‑1 (MQ=255)

                 aCTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑ggg‑‑g                                                                                                                               >  1:2381061/1‑127 (MQ=255)

                   tGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGAt                                                                                                                <  2:2147992/140‑1 (MQ=255)

                   tGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGAt                                                                                                                <  1:186739/140‑1 (MQ=255)

                   tGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGAt                                                                                                                >  2:1708562/1‑140 (MQ=255)

                   tGCCGCGCGTAGGCGCAAAGGGATTCAACATAACGGCTCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGAt                                                                                                                <  1:1152194/140‑1 (MQ=255)

                   tGCCGCGCGTAGGCGAAAAGGGATTCAACCTAACGGCCCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGAt                                                                                                                <  1:627886/140‑1 (MQ=255)

                     ccGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑‑GG‑‑GGATAACGAGGTTGATGtt                                                                                                             >  2:253387/1‑140 (MQ=255)

                        cgcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttt                                                                                                            >  1:420419/1‑139 (MQ=255)

                        cgcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttt                                                                                                            <  2:420419/139‑1 (MQ=255)

                         gcgTAGGCGGAAAGGTATTCAATGTGACGCCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  1:690391/140‑1 (MQ=255)

                         gcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  2:1299394/140‑1 (MQ=255)

                         gcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  2:1202357/140‑1 (MQ=255)

                         gcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  2:2279356/140‑1 (MQ=255)

                         gcgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  2:98457/140‑1 (MQ=255)

                         gcgTAGGCGGAAAGGCATTCAACGTCACGCCGCTGCCATTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  1:632527/140‑1 (MQ=255)

                          cgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          <  2:2424293/139‑1 (MQ=255)

                          cgTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGttttt                                                                                                          >  1:2424293/1‑139 (MQ=255)

                               gCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGAtt                                                                                                    >  2:2323116/1‑140 (MQ=255)

                               gCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGAtt                                                                                                    <  2:2348782/140‑1 (MQ=255)

                                cGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTa                                                                                                   <  2:1996931/140‑1 (MQ=255)

                                         aTTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑AGATAACGAGGTGGAGGTTTTTGAGATTATGGGTGCgg                                                                                          >  1:915690/1‑140 (MQ=255)

                                               cGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGccc                                                                                    >  1:610491/1‑140 (MQ=255)

                                                 tAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCg                                                                                  >  2:428182/1‑140 (MQ=255)

                                                      gcgcTGCCCTTCGGCATATAAGGTGTCGAAGGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACtt                                                                             <  2:1526497/140‑1 (MQ=255)

                                                      gcgcTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACtt                                                                             <  1:1498426/140‑1 (MQ=255)

                                                      gcgcTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTAGAGGTTATGGGTGGGGGCGCCCCGAACtt                                                                             >  1:769377/1‑140 (MQ=255)

                                                      gcgcTGCCCTTCGGCATATAAGCTGCCGACAGCGAGCCAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACtt                                                                             <  1:739724/140‑1 (MQ=255)

                                                       cgcTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGcccc                                                                                   >  1:309824/1‑133 (MQ=255)

                                                        gcTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCg                                                                           <  1:1708562/140‑1 (MQ=255)

                                                         cTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATATCGAGGTTGATGTATTTGAGATTATGGGTGCAGGCGCCCCGAACTTCGa                                                                          >  2:892150/1‑140 (MQ=255)

                                                         cTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGa                                                                          >  1:160350/1‑140 (MQ=255)

                                                                tCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATc                                                                   >  1:1579485/1‑140 (MQ=255)

                                                                 cGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATcc                                                                  >  2:2015135/1‑140 (MQ=255)

                                                                 cGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATcc                                                                  >  2:1826531/1‑140 (MQ=255)

                                                                  ggCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCa                                                                 <  2:1439865/140‑1 (MQ=255)

                                                                      tataAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCtt                                                                      >  1:1342923/1‑131 (MQ=255)

                                                                      tataAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCa                                                             >  1:2429294/1‑140 (MQ=255)

                                                                      tataAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCa                                                             >  2:503977/1‑140 (MQ=255)

                                                                      tataAGGTGTCGAAAGCGAGCGAGGATTTCCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCa                                                             >  2:479710/1‑140 (MQ=255)

                                                                       ataAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCAc                                                            <  2:610491/140‑1 (MQ=255)

                                                                       ataAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCAc                                                            >  1:488564/1‑140 (MQ=255)

                                                                        taAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCAcc                                                           >  1:220850/1‑140 (MQ=255)

                                                                        taAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGAGTGCGGGGGCCTCGAACTTCGGTCTTATCCATTCAcc                                                           >  1:1002101/1‑140 (MQ=255)

                                                                           ggTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCttt                                                        >  1:2170199/1‑140 (MQ=255)

                                                                           ggTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCttt                                                        >  1:1720409/1‑140 (MQ=255)

                                                                             tgtCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTcc                                                      >  1:1996165/1‑140 (MQ=255)

                                                                             tgtCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAAATTCGATCTTTTCCATTCACCTTTcc                                                      >  1:969021/1‑140 (MQ=255)

                                                                              gtCGAAAGCGAGCGAGGTTTTGACAGAACCCAAAAACCCCGTCACGACCATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTccc                                                     <  2:699503/140‑1 (MQ=255)

                                                                              gtCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTccc                                                     >  2:1970281/1‑140 (MQ=255)

                                                                              gtCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTccc                                                     >  1:202951/1‑140 (MQ=255)

                                                                               tCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCg                                                    >  2:1640124/1‑140 (MQ=255)

                                                                               tCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCg                                                    >  2:128270/1‑140 (MQ=255)

                                                                               tCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCg                                                    >  2:1863004/1‑140 (MQ=255)

                                                                               tCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGCCGCCCCGAACTCCGCTCTTATGCATTCACCTTTCCCg                                                    >  1:1085678/1‑140 (MQ=255)

                                                                               tCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGACGCCCCGCACTTCGATCTTATACATTCACCTTTCCCg                                                    >  1:1043787/1‑140 (MQ=255)

                                                                                cGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCgg                                                   >  1:1375963/1‑140 (MQ=255)

                                                                                            agGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑‑GG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCG‑CCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCttttt                                     <  1:253387/140‑1 (MQ=255)

                                                                                             ggATTTGCCAGAACCTGAAAGCCCGATCACGACAATGCGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCtttt                                      <  1:984855/140‑1 (MQ=255)

                                                                                                tttGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGCGCGGGCGCCCCGAACTTCGATGTTATCCAATCAACTGTCCCGGCTTAAACGCTTTTTTg                                   >  1:1080155/1‑140 (MQ=255)

                                                                                                   gCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGccc                                >  1:2301608/1‑140 (MQ=255)

                                                                                                        aaCCCGAAAGCCCGGTCACGACAATGAGCTTGCCGCGGGGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTACGATCTTAGCCAGTCAGCTTTCCCGGATTAGACGCTTTTTTGCCCGGt                             >  2:668646/1‑140 (MQ=255)

                                                                                                        aaCCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCgg                              <  2:573218/137‑1 (MQ=255)

                                                                                                        aaCCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCgg                              >  1:573218/1‑137 (MQ=255)

                                                                                                        aaCCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTgg                           >  1:2363698/1‑140 (MQ=255)

                                                                                                         aCCCGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGc                          <  1:428182/140‑1 (MQ=255)

                                                                                                          cccGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCa                         <  1:506329/140‑1 (MQ=255)

                                                                                                          cccGAAAGCCCGGTCACCACAAAGAGCTTGCCGC‑‑GGG‑‑GGTTAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCa                         <  2:1043787/140‑1 (MQ=255)

                                                                                                           ccGAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCAt                        <  2:220850/140‑1 (MQ=255)

                                                                                                             gAAAGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGAATTAAAGGCTTTTATGCCCGGTGGCATgg                      >  1:868542/1‑140 (MQ=255)

                                                                                                               aaGCCCGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTg                    <  1:831532/140‑1 (MQ=255)

                                                                                                                  cccGGTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTa                 >  2:285986/1‑140 (MQ=255)

                                                                                                                     ggTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCg              >  2:589706/1‑140 (MQ=255)

                                                                                                                     ggTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCg              >  1:1574915/1‑140 (MQ=255)

                                                                                                                     ggTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCg              >  2:1763106/1‑140 (MQ=255)

                                                                                                                      gTCACGACAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCgg             >  2:501242/1‑140 (MQ=255)

                                                                                                                          cGACAATGAGCTTGCAGC‑‑GGGGCGGTTAACGAGGTTGCTGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCg           <  1:668646/140‑1 (MQ=255)

                                                                                                                            aCAATGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTCTGTTGCCCGGTGGCATGGTGCTACCGGCGATca       >  2:1059789/1‑140 (MQ=255)

                                                                                                                              aaTGAGCTTGCCGC‑‑GGG‑‑GGATAACGCGGTCGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATcaca     <  1:1172029/140‑1 (MQ=255)

                                                                                                                              aaTGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATcaca     <  1:1863004/140‑1 (MQ=255)

                                                                                                                                tGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCTATCTTATCCATTCACCTTTCCCGCATTAAACGCTTTTTTGGCCGGTGGCTTGGTGCTACCGGCGATCACaaa   >  2:840659/1‑140 (MQ=255)

                                                                                                                                tGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGTATTAAACGATTTGTTGACCGGTGGCTTGGTCCTACCGGCGATCACaaa   >  2:951214/1‑140 (MQ=255)

                                                                                                                                tGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACaaa   >  1:11291/1‑140 (MQ=255)

                                                                                                                                tGAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACaaa   <  1:2271653/140‑1 (MQ=255)

                                                                                                                                agAGCTTGCCGC‑‑GGG‑‑CGATAACTAGATTGGTGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACaaa   <  1:1012186/139‑1 (MQ=255)

                                                                                                                                 gAGCTTGCCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACAAAc  <  1:2021835/140‑1 (MQ=255)

TTCCATCAGTGACAGAAACTGCCGCGCGTAGGCGGAAAGGGATTCAACGTAACGGCGCTGCCCTTCGGCATATAAGGTGTCGAAAGCGAGCGAGGATTTGCCAGAACCCGAAAGCCCGGTCACGACAATGAGCTTGTCGC‑‑GGG‑‑GGATAACGAGGTTGATGTTTTTGAGATTATGGGTGCGGGCGCCCCGAACTTCGATCTTATCCATTCACCTTTCCCGGATTAAACGCTTTTTTGCCCGGTGGCATGGTGCTACCGGCGATCACAAAC  >  NC_000913/4273665‑4273933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A