Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A41 F60 I1 R1	13	41.9	1423038	99.2%	1411653	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,400,694	G→A	G8D (GGT→GAT)	hflX →	ribosome rescue factor HflX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,694	0	G	A	100.0%	179.3 / NA	50	G8D (GGT→GAT)	hflX	ribosome rescue factor HflX
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/33); total (17/33)

ATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCC  >  NC_000913/4400559‑4400826

aTACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAg                                                                                                                                  <  1:377248/140‑1 (MQ=255)

aTACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAg                                                                                                                                  <  2:532296/140‑1 (MQ=255)

    ttCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAgg                                                                                                                              <  1:577777/140‑1 (MQ=255)

    ttCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAgg                                                                                                                              <  1:352243/140‑1 (MQ=255)

     tCCGCGCAACAGGACAGCGAATAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGc                                                                                                                             <  2:647375/140‑1 (MQ=255)

     tCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGc                                                                                                                             <  2:240646/140‑1 (MQ=255)

     tCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGc                                                                                                                             <  2:318304/140‑1 (MQ=255)

        gcgcAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAgg                                                                                                                              <  2:689380/136‑1 (MQ=255)

        gcgcAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAgg                                                                                                                              >  1:689380/1‑136 (MQ=255)

        gcgcAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGt                                                                                                                          <  2:708574/140‑1 (MQ=255)

             aCAGGACAGCGAAGAAACCGACTAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTgg                                                                                                                     <  2:647338/140‑1 (MQ=255)

              cAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGc                                                                                                                             <  1:650283/131‑1 (MQ=255)

              cAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGc                                                                                                                             >  2:650283/1‑131 (MQ=255)

              cAGGACAGCGAAG‑AACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTa                                                                                                                   <  1:319952/140‑1 (MQ=255)

               aGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTa                                                                                                                   <  1:330825/140‑1 (MQ=255)

               aGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTa                                                                                                                   <  2:616741/140‑1 (MQ=255)

                      cGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCt                                                                                                            >  1:34243/1‑140 (MQ=255)

                         agaaACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTAtt                                                                                                         <  2:238848/140‑1 (MQ=255)

                                gAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCa                                                                                                  >  2:303982/1‑140 (MQ=255)

                                    aaGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGAc                                                                                              <  1:120475/140‑1 (MQ=255)

                                    aaGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGAc                                                                                              <  1:210770/140‑1 (MQ=255)

                                     aGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACa                                                                                             <  2:206941/140‑1 (MQ=255)

                                           cGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCGATTTTACGCAAGACAAAGata                                                                                       >  2:647369/1‑140 (MQ=255)

                                                 gTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAg                                                                                 <  2:34243/140‑1 (MQ=255)

                                                         acacGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAg                                                                         <  1:394994/140‑1 (MQ=255)

                                                           acGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAAGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGa                                                                       <  2:263267/140‑1 (MQ=255)

                                                               ggAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGttt                                                                   >  1:192485/1‑140 (MQ=255)

                                                                     aGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAAtct                                                             <  2:489613/140‑1 (MQ=255)

                                                                                  tCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTccg                                                <  1:267543/140‑1 (MQ=255)

                                                                                   ccccGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTccgc                                               >  1:44259/1‑140 (MQ=255)

                                                                                    cccGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTccgcc                                              >  1:300771/1‑140 (MQ=255)

                                                                                    cccGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGGACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTccgcc                                              >  1:221865/1‑140 (MQ=255)

                                                                                            tCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGa                                      <  1:303982/140‑1 (MQ=255)

                                                                                            tCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGa                                      <  2:44259/140‑1 (MQ=255)

                                                                                                tttAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCa                                  <  2:292596/140‑1 (MQ=255)

                                                                                                   agagGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTg                               <  2:173158/140‑1 (MQ=255)

                                                                                                     agGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGa                                      <  2:597417/131‑1 (MQ=255)

                                                                                                     agGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGa                                      >  1:597417/1‑131 (MQ=255)

                                                                                                     agGGTTATACGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCa                             >  1:669786/1‑140 (MQ=255)

                                                                                                           ataCGCTTGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGAt                       >  2:455832/1‑140 (MQ=255)

                                                                                                                 ttGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCgg                 <  1:647369/140‑1 (MQ=255)

                                                                                                                  tGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGt                >  1:422820/1‑140 (MQ=255)

                                                                                                                  tGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGt                <  2:300771/140‑1 (MQ=255)

                                                                                                                  tGTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGt                <  2:669786/140‑1 (MQ=255)

                                                                                                                   gTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGc                              >  2:265704/1‑125 (MQ=255)

                                                                                                                   gTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGc                              <  1:265704/125‑1 (MQ=255)

                                                                                                                   gTTTGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTa               >  2:31554/1‑140 (MQ=255)

                                                                                                                   gTTTGACCGTTATGATGCTGATGAGCAAGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTa               >  2:236231/1‑140 (MQ=255)

                                                                                                                    tttGACCGTTATGATGCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAg              >  2:226245/1‑140 (MQ=255)

                                                                                                                                gatgCTGATGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGcgcc  <  2:221865/140‑1 (MQ=255)

ATACTTCCGCGCAACAGGACAGCGAAGAAACCGAATAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCC  >  NC_000913/4400559‑4400826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A