Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	23	45.9	1548470	99.2%	1536082	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,939	A→C	I15M (ATT→ATG)	ytfE ←	iron‑sulfur cluster repair protein YtfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,939	0	A	C	100.0%	123.9 / NA	33	I15M (ATT→ATG)	ytfE	iron‑sulfur cluster repair protein YtfE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (15/18); total (15/18)

CGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTT  >  NC_000913/4431809‑4432073

cGAGCTTTGCCAGTTCACCTTCAATGACCTCCACAACCAGTTCTTTACCTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAgc                                                                                                                               <  2:288792/140‑1 (MQ=255)

  aGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGTGCGAGGCATAGAGAgcgc                                                                                                                             >  1:412837/1‑140 (MQ=255)

       tGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGtt                                                                                                                        <  1:517520/140‑1 (MQ=255)

       tGCCAGTTCAGCTTCAAGGCCCTCAACATCCTGTTTTTTTCGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGtt                                                                                                                        <  2:325666/140‑1 (MQ=255)

                      aaTGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGt                                                                                                         >  2:729654/1‑140 (MQ=255)

                               aaCTTCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGa                                                                                                    <  2:85295/136‑1 (MQ=255)

                               aaCATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGa                                                                                                    >  1:85295/1‑136 (MQ=255)

                                 cATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGcc                                                                                              <  1:669266/140‑1 (MQ=255)

                                  aTCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCa                                                                                             <  2:617841/140‑1 (MQ=255)

                                      aGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGc                                                                                         >  1:594879/1‑140 (MQ=255)

                                         tCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGa                                                                                                    >  1:698386/1‑126 (MQ=255)

                                         tCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGa                                                                                                    <  2:698386/126‑1 (MQ=255)

                                            ttACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAc                                                                                   >  2:176460/1‑140 (MQ=255)

                                             tACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCa                                                                                             <  1:11178/129‑1 (MQ=255)

                                             tACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCa                                                                                             >  2:11178/1‑129 (MQ=255)

                                             tACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                                                                                  >  2:549500/1‑140 (MQ=255)

                                             tACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                                                                                  <  1:176460/140‑1 (MQ=255)

                                                  gccgccGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAtt                                                                             >  2:513849/1‑140 (MQ=255)

                                                             cgcCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagat                                                                  <  1:729654/140‑1 (MQ=255)

                                                                cAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagataag                                                               <  1:549500/140‑1 (MQ=255)

                                                                  gCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGTGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATaagataagat                                                             <  2:412837/140‑1 (MQ=255)

                                                                            aCCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTaaa                                                   <  1:513849/140‑1 (MQ=255)

                                                                             ccGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAAt                                                  <  2:594879/140‑1 (MQ=255)

                                                                              cGCCACAGCAGTAATGCATATCGTACTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATg                                                 <  1:324764/140‑1 (MQ=255)

                                                                                                     tatTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATTAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATaa                          >  2:407287/1‑140 (MQ=255)

                                                                                                     tatTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATaa                          >  2:703948/1‑140 (MQ=255)

                                                                                                      atTTACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAAc                         >  2:163815/1‑140 (MQ=255)

                                                                                                        ttACGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAAccc                       <  1:14615/140‑1 (MQ=255)

                                                                                                           cGAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTa                    <  1:163815/140‑1 (MQ=255)

                                                                                                            gAAACAGAGCTGAAGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATTAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTAc                   <  1:407287/140‑1 (MQ=255)

                                                                                                                        aaGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTGAAGCCAAAAGCTATAACACTTACGTAGCAGAGGCt       >  2:265355/1‑140 (MQ=255)

                                                                                                                        aaGCGCGAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCCTTAAGGCAAAACCTTTAACCCTTACGTAGCAGAGGCt       >  1:251752/1‑140 (MQ=255)

                                                                                                                             cgAGGCATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTAttt  >  1:727954/1‑140 (MQ=255)

CGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTT  >  NC_000913/4431809‑4432073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A