Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 124 90.1 3003320 99.2% 2979293 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,423,417 C→T A191V (GCG→GTG)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,423,4170CT99.0% 330.0 / NA 98A191V (GCG→GTG) ulaEL‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (55/42);  total (56/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTT  >  NC_000913/4423284‑4423551
                                                                                                                                     |                                                                                                                                      
cgcgcgCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGa                                                                                                                                  <  2:1410735/140‑1 (MQ=255)
cgcgcgCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGa                                                                                                                                  <  2:133272/140‑1 (MQ=255)
     gcAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACg                                                                                                                             <  2:708673/140‑1 (MQ=255)
       aGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGAt                                                                                                                           <  1:569581/140‑1 (MQ=255)
        ggTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGAtg                                                                                                                          <  1:33684/140‑1 (MQ=255)
         gTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGAtgt                                                                                                                         <  2:1150940/140‑1 (MQ=255)
         gTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGAtgt                                                                                                                         <  1:1409255/140‑1 (MQ=255)
           gACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGc                                                                                                                       <  1:1257776/140‑1 (MQ=255)
              gCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGa                                                                                                                    <  2:964428/140‑1 (MQ=255)
                   cGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAg                                                                                                               >  2:1036251/1‑140 (MQ=255)
                   cGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAg                                                                                                               >  2:637540/1‑140 (MQ=255)
                       ggAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGc                                                                                                           >  1:1180358/1‑140 (MQ=255)
                       ggAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGc                                                                                                           >  2:918191/1‑140 (MQ=255)
                        gagaTCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCa                                                                                                          <  2:204282/140‑1 (MQ=255)
                         agaTCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCATCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAg                                                                                                         >  2:1333844/1‑140 (MQ=255)
                           aTCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGc                                                                                                       <  2:1261635/140‑1 (MQ=255)
                            tCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGcc                                                                                                      >  1:1186460/1‑140 (MQ=255)
                            tCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGcc                                                                                                      >  1:832410/1‑140 (MQ=255)
                               tGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGa                                                                                                   <  1:899173/140‑1 (MQ=255)
                                ggATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGaa                                                                                                  <  2:393150/140‑1 (MQ=255)
                                 gATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAAt                                                                                                 <  1:645920/140‑1 (MQ=255)
                                  aTTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATc                                                                                                >  2:286419/1‑140 (MQ=255)
                                  aTTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATc                                                                                                <  1:537846/140‑1 (MQ=255)
                                   ttATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCg                                                                                               >  2:957220/1‑140 (MQ=255)
                                      tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGc                                                                                            >  1:20568/1‑140 (MQ=255)
                                      tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGc                                                                                            >  2:684551/1‑140 (MQ=255)
                                      tCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGc                                                                                            >  1:70090/1‑140 (MQ=255)
                                       ccGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCa                                                                                           >  1:635575/1‑140 (MQ=255)
                                          ttGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCatat                                                                                        <  2:379571/140‑1 (MQ=255)
                                          ttGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCatat                                                                                        >  1:93763/1‑140 (MQ=255)
                                             atgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCTGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                                                                                     <  2:1405200/140‑1 (MQ=255)
                                             atgaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgt                                                                                     <  1:924623/140‑1 (MQ=255)
                                               gaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg                                                                                   <  1:188866/140‑1 (MQ=255)
                                               gaGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtcgtcg                                                                                   <  1:909929/140‑1 (MQ=255)
                                                aGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgc                                                                                  <  1:1236861/140‑1 (MQ=255)
                                                 gCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgcg                                                                                 <  2:1410248/140‑1 (MQ=255)
                                                 gCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgcg                                                                                 <  1:842937/140‑1 (MQ=255)
                                                 gCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgcg                                                                                 <  2:171868/140‑1 (MQ=255)
                                                  cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg                                                                                <  1:72092/140‑1 (MQ=255)
                                                  cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg                                                                                >  2:771645/1‑140 (MQ=255)
                                                  cTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCgg                                                                                >  2:1276505/1‑140 (MQ=255)
                                                    ccATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTa                                                                              >  2:166460/1‑140 (MQ=255)
                                                     cATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTAc                                                                             <  1:1332086/140‑1 (MQ=255)
                                                     cATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTAc                                                                             <  2:927431/140‑1 (MQ=255)
                                                           cAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGa                                                                       >  2:501963/1‑140 (MQ=255)
                                                            aaGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaa                                                                      >  2:250459/1‑140 (MQ=255)
                                                             aGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa                                                                     >  2:915171/1‑140 (MQ=255)
                                                                  cTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGacac                                                                >  1:144470/1‑140 (MQ=255)
                                                                  cTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGacac                                                                <  1:1052326/140‑1 (MQ=255)
                                                                     ggATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCaa                                                             >  2:391937/1‑140 (MQ=255)
                                                                         aCGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCt                                                         >  2:972307/1‑140 (MQ=255)
                                                                          cgcgCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGACATATCGTCGCGGTACATGTGAAAGACACCAAACCTg                                                        >  1:122180/1‑140 (MQ=255)
                                                                            cgcACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGc                                                      <  2:832410/140‑1 (MQ=255)
                                                                            cgcACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGc                                                      <  1:684551/140‑1 (MQ=255)
                                                                               aCTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCGTATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTc                                                   >  1:1251357/1‑140 (MQ=255)
                                                                               aCTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTc                                                   >  2:655918/1‑140 (MQ=255)
                                                                                   tctcAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCa                                               >  2:1322379/1‑140 (MQ=255)
                                                                                   tctcAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCa                                               >  2:426869/1‑140 (MQ=255)
                                                                                         caaTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACg                                         >  2:919498/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  2:739244/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  1:546877/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       <  1:286419/140‑1 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  1:719488/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  1:766523/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  1:100957/1‑140 (MQ=255)
                                                                                           aTCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGCCTTCAAAAACGTg                                       >  2:1459325/1‑140 (MQ=255)
                                                                                           aTCCGTGGCTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTg                                       >  2:290782/1‑140 (MQ=255)
                                                                                            tCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGc                                      >  1:367696/1‑140 (MQ=255)
                                                                                             ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                     >  1:823872/1‑140 (MQ=255)
                                                                                             ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                     >  2:291175/1‑140 (MQ=255)
                                                                                             ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                     >  2:750175/1‑140 (MQ=255)
                                                                                             ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                     >  2:699739/1‑140 (MQ=255)
                                                                                             ccGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGcc                                     >  2:624198/1‑140 (MQ=255)
                                                                                                     ccAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCg                             <  1:771645/140‑1 (MQ=255)
                                                                                                     ccAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCg                             <  2:137681/140‑1 (MQ=255)
                                                                                                      cAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGa                            <  2:20568/140‑1 (MQ=255)
                                                                                                      cAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGa                            <  1:128904/140‑1 (MQ=255)
                                                                                                      cAGCTCTACCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGa                            >  2:752808/1‑140 (MQ=255)
                                                                                                             aCCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                     >  1:1166842/1‑140 (MQ=255)
                                                                                                             aCCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                     >  1:1711/1‑140 (MQ=255)
                                                                                                             aCCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                     >  1:749259/1‑140 (MQ=255)
                                                                                                             aCCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                     >  2:1040783/1‑140 (MQ=255)
                                                                                                             aCCCGGATATCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTa                     >  2:829629/1‑140 (MQ=255)
                                                                                                                   atatCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGAt               >  1:674409/1‑140 (MQ=255)
                                                                                                                   atatCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGAt               <  1:250459/140‑1 (MQ=255)
                                                                                                                   atatCGGCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGAt               >  1:445005/1‑140 (MQ=255)
                                                                                                                        ggCAACCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGa          >  1:1490296/1‑140 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  1:637540/140‑1 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  2:367522/140‑1 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  1:1357858/140‑1 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  1:291175/140‑1 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  2:1460434/140‑1 (MQ=255)
                                                                                                                           aaCCTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACg       <  1:665064/140‑1 (MQ=255)
                                                                                                                             ccTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt     >  2:30557/1‑140 (MQ=255)
                                                                                                                             ccTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt     >  1:1232741/1‑140 (MQ=255)
                                                                                                                             ccTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt     >  2:1332553/1‑140 (MQ=255)
                                                                                                                             ccTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt     >  2:452052/1‑140 (MQ=255)
                                                                                                                             ccTGTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGCGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt     >  2:389013/1‑140 (MQ=255)
                                                                                                                                gTCGGTGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgttgtt  <  1:750175/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTT  >  NC_000913/4423284‑4423551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A