Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,326,444	A→G	G97G (GGT→GGC)	yjdM ←	conserved protein YjdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,444	0	A	G	100.0%	193.4 / NA	51	G97G (GGT→GGC)	yjdM	conserved protein YjdM
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/21); total (30/21)

ATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTCAGCCA  >  NC_000913/4326311‑4326580

aTAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCg                                                                                                                                    >  1:143438/1‑140 (MQ=255)

      cgcgCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTg                                                                                                                              >  1:514449/1‑140 (MQ=255)

          cAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAAt                                                                                                                          >  2:807471/1‑140 (MQ=255)

              cGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGAt                                                                                                                      >  1:281054/1‑140 (MQ=255)

               gTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATg                                                                                                                     <  1:396994/140‑1 (MQ=255)

                      cAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGt                                                                                                              <  1:398743/140‑1 (MQ=255)

                          cATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGcc                                                                                                          <  2:87556/140‑1 (MQ=255)

                          cATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGcc                                                                                                          <  1:898669/140‑1 (MQ=255)

                                gCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAAc                                                                                                    >  1:870763/1‑140 (MQ=255)

                                gCACCGTTGCCGGATGCGGCGCGAGCACCTTATCCGGCTTACCGATGACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAAc                                                                                                    >  2:832878/1‑140 (MQ=255)

                                   ccGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAg                                                                                                 >  1:227401/1‑140 (MQ=255)

                                   ccGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAg                                                                                                 >  1:58092/1‑140 (MQ=255)

                                      ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  1:925533/1‑140 (MQ=255)

                                      ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  1:905716/1‑140 (MQ=255)

                                      ttGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCg                                                                                              >  2:764582/1‑140 (MQ=255)

                                       tGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGCTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCgg                                                                                             >  1:777526/1‑140 (MQ=255)

                                              tGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTACACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGtttt                                                                                      >  1:603813/1‑140 (MQ=255)

                                                   cgcgAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACt                                                                                 >  2:378762/1‑140 (MQ=255)

                                                            cTTATCCGGCTTACAGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCTACGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTTGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCa                                                                        <  2:908813/140‑1 (MQ=255)

                                                                   ggCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCa                                                                 <  2:744772/140‑1 (MQ=255)

                                                                   ggCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCa                                                                 <  2:143438/140‑1 (MQ=255)

                                                                      ttACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCa                                                              >  2:143892/1‑140 (MQ=255)

                                                                        aCCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATc                                                            >  2:838537/1‑140 (MQ=255)

                                                                        aCCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATc                                                            >  1:346492/1‑140 (MQ=255)

                                                                           gATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGaa                                                         >  2:448082/1‑140 (MQ=255)

                                                                                caatacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCt                                                    <  2:386551/140‑1 (MQ=255)

                                                                                   tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  2:556257/1‑140 (MQ=255)

                                                                                   tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  1:100943/1‑140 (MQ=255)

                                                                                   tacaatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTAcc                                                 >  1:697764/1‑140 (MQ=255)

                                                                                      aatCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCttt                                              <  1:828027/140‑1 (MQ=255)

                                                                                        tCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCa                                            >  1:883069/1‑140 (MQ=255)

                                                                                        tCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCa                                            >  1:309140/1‑140 (MQ=255)

                                                                                          aGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCAcc                                          <  1:838537/140‑1 (MQ=255)

                                                                                           gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:1009131/1‑140 (MQ=255)

                                                                                           gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:494192/1‑140 (MQ=255)

                                                                                           gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  2:207539/1‑140 (MQ=255)

                                                                                           gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  1:594220/1‑140 (MQ=255)

                                                                                           gTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCt                                         >  2:182965/1‑140 (MQ=255)

                                                                                            ttCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCtt                                        <  2:227401/140‑1 (MQ=255)

                                                                                             tCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTc                                       <  1:764582/140‑1 (MQ=255)

                                                                                              cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:66899/140‑1 (MQ=255)

                                                                                              cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:925533/140‑1 (MQ=255)

                                                                                              cTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCa                                      <  2:514449/140‑1 (MQ=255)

                                                                                               ttttaCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAg                                     <  2:603813/140‑1 (MQ=255)

                                                                                                   tCACAAACTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCt                                 <  1:832878/140‑1 (MQ=255)

                                                                                                        aaCTCAGATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAat                            <  1:532889/140‑1 (MQ=255)

                                                                                                              gATTTCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGt                      <  2:281054/140‑1 (MQ=255)

                                                                                                                  tCAGTTTCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACg                  <  2:346492/140‑1 (MQ=255)

                                                                                                                       ttCATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTc             >  1:455989/1‑140 (MQ=255)

                                                                                                                         cATCGGACCAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGc           >  1:460031/1‑140 (MQ=255)

                                                                                                                                ccAAAGCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTcagc    <  2:178605/140‑1 (MQ=255)

                                                                                                                                  aaagCCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTcagcca  <  2:870763/140‑1 (MQ=255)

ATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAGCTACCTTTCACCTTCAGATCTTTAATGATGGTAACGCTGTCGCCGTCAGCCA  >  NC_000913/4326311‑4326580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A