Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
132 |
61.4 |
2079690 |
99.1% |
2060972 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,472,714 |
T→C |
intergenic (‑181/+100) |
pyrL ← / ← yjgH |
pyrBI operon leader peptide/RutC family protein YjgH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,472,714 | 0 | T | C | 98.1%
| 197.5
/ ‑3.9
| 53 | intergenic (‑181/+100) | pyrL/yjgH | pyrBI operon leader peptide/RutC family protein YjgH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (22/30); minor base A (0/1); total (22/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
GCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACTT > NC_000913/4472581‑4472842
|
gcgcAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttt < 2:531949/140‑1 (MQ=255)
cAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcc < 2:497259/140‑1 (MQ=255)
cAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcc > 1:381542/1‑140 (MQ=255)
aGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGctttttcctt > 2:85390/1‑140 (MQ=255)
aaTATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTtttcactttcac < 1:671380/140‑1 (MQ=255)
atatTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACg > 1:759559/1‑140 (MQ=255)
atTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCa < 1:113335/140‑1 (MQ=255)
gTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAg < 1:1037865/140‑1 (MQ=255)
gTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAg < 1:468341/140‑1 (MQ=255)
cAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGat > 1:345536/1‑140 (MQ=255)
gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat < 2:644593/140‑1 (MQ=255)
gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat < 2:218556/140‑1 (MQ=255)
gcgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGatat > 1:502114/1‑140 (MQ=255)
cgcAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATg > 1:881357/1‑140 (MQ=255)
aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 1:51363/140‑1 (MQ=255)
aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 1:216182/140‑1 (MQ=255)
aaTTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCAGCTTTTTCCTTTTTCTCTTTCACGCAATCAGATATGCATTTTATTCCATTCAt < 2:586258/140‑1 (MQ=255)
ttAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATcc < 1:99592/140‑1 (MQ=255)
tAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCg < 1:172652/140‑1 (MQ=255)
tCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTtat < 2:898440/140‑1 (MQ=255)
ggTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAggg < 1:116875/140‑1 (MQ=255)
tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:733958/140‑1 (MQ=255)
tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 2:827900/140‑1 (MQ=255)
tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:1027681/140‑1 (MQ=255)
tataTGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCg < 1:30974/140‑1 (MQ=255)
tttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGa > 1:651130/1‑140 (MQ=255)
ttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGaa > 2:618147/1‑140 (MQ=255)
ttttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGaa > 2:608053/1‑140 (MQ=255)
tttATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTTTAGGGCGGAGTGTGaaa > 2:364937/1‑140 (MQ=255)
tATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGTGGAGTGTGAAAGc < 1:1434/140‑1 (MQ=255)
tATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGc > 1:881868/1‑140 (MQ=255)
aTTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCg < 2:502114/140‑1 (MQ=255)
tttGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGa < 1:334659/140‑1 (MQ=255)
tttGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGa < 2:46729/140‑1 (MQ=255)
aTGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACgg > 1:360434/1‑140 (MQ=255)
tgtgATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCGCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGc > 1:238621/1‑140 (MQ=255)
gtgATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCt < 2:273266/140‑1 (MQ=255)
gATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTaa > 1:67765/1‑140 (MQ=255)
gATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTaa > 1:113296/1‑140 (MQ=255)
ttCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAaca < 1:85390/140‑1 (MQ=255)
gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:381542/140‑1 (MQ=255)
gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:113296/140‑1 (MQ=255)
gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:881868/140‑1 (MQ=255)
gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:601396/140‑1 (MQ=255)
gAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTa < 2:67765/140‑1 (MQ=255)
cTGTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTg > 2:374005/1‑140 (MQ=255)
gTCCTTCGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCt > 1:1038658/1‑140 (MQ=255)
cGGTATCAATAATTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGa > 2:43334/1‑140 (MQ=255)
aataatTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATgcgcgc > 2:558077/1‑140 (MQ=255)
aatTGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGCAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTAt < 2:759559/140‑1 (MQ=255)
ttGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCa > 2:861061/1‑140 (MQ=255)
tGCAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCAc > 1:724908/1‑140 (MQ=255)
cAGACATTCCCCGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACtt > 1:782417/1‑140 (MQ=255)
|
GCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATGCATTTTTATTTGATGTGATTCTGCAGGGGAACTGTCCTTCGGTATCAATAATTGCAGACATTCCCTGCTTTTTCCTTTTTCACTTTCACGCAATCAGATATGCATTTTATTCCATTCATCCGACTTATAGGGCGGAGTGTGAAAGCGAACGGCTAACACTATTGCTTACTGCTCAGGGATGCGCGCTATCACTT > NC_000913/4472581‑4472842
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
