Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,286	C→T	A42T (GCC→ACC)	fecC ←	ferric citrate ABC transporter membrane subunit FecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,286	0	C	T	100.0%	119.2 / NA	37	A42T (GCC→ACC)	fecC	ferric citrate ABC transporter membrane subunit FecC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/18); total (19/18)

TTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCAT  >  NC_000913/4513150‑4513409

ttGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGtgcg                                                                                                                          <  2:391817/140‑1 (MQ=255)

 tgtgGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCgg                                                                                                                         >  2:186178/1‑140 (MQ=255)

        cAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCAt                                                                                                                  >  2:491832/1‑140 (MQ=255)

        cAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCAt                                                                                                                  >  1:734682/1‑140 (MQ=255)

         aGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATc                                                                                                                 >  2:568814/1‑140 (MQ=255)

             tttGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCt                                                                                                             <  2:356543/140‑1 (MQ=255)

                  agcagcGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGa                                                                                                        >  2:719368/1‑140 (MQ=255)

                     agcGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAAc                                                                                                     >  2:65237/1‑140 (MQ=255)

                         tGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGa                                                                                                 >  2:401461/1‑140 (MQ=255)

                                gAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCg                                                                                          <  2:991925/140‑1 (MQ=255)

                                          cTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCaaaa                                                                                <  2:986824/140‑1 (MQ=255)

                                              cgcCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGa                                                                            <  1:931887/140‑1 (MQ=255)

                                              cgcCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGa                                                                            >  1:365962/1‑140 (MQ=255)

                                                   aTCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAg                                                                       >  1:768946/1‑140 (MQ=255)

                                                   aTCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAg                                                                       >  2:756378/1‑140 (MQ=255)

                                                   aTCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAg                                                                       >  1:268908/1‑140 (MQ=255)

                                                    tCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGc                                                                      <  1:826889/140‑1 (MQ=255)

                                                    tCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTGGGGGTGTGTCCAGGCAGCTGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACGCAGc                                                                      <  2:243563/140‑1 (MQ=255)

                                                        aaCGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGa                                                                  <  2:84225/140‑1 (MQ=255)

                                                        aaCGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGa                                                                  <  1:568814/140‑1 (MQ=255)

                                                        aaCGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGa                                                                  <  2:673732/140‑1 (MQ=255)

                                                           ggCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGaaaa                                                               <  1:719368/140‑1 (MQ=255)

                                                             cGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAaata                                                             <  2:975757/140‑1 (MQ=255)

                                                             cGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAaata                                                             <  2:858946/140‑1 (MQ=255)

                                                                  aGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAaataataat                                                        >  1:285275/1‑140 (MQ=255)

                                                                           ggCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGtgctgc                                               >  1:48921/1‑140 (MQ=255)

                                                                                aCGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACgg                                          <  2:769277/140‑1 (MQ=255)

                                                                                   aaGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGaa                                       <  1:962823/140‑1 (MQ=255)

                                                                                      gTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGcc                                    >  2:805649/1‑140 (MQ=255)

                                                                                        tttGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGcccc                                  <  2:952670/140‑1 (MQ=255)

                                                                                                      tCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCgg                    <  2:933072/140‑1 (MQ=255)

                                                                                                      tCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCgg                    >  2:540418/1‑140 (MQ=255)

                                                                                                       cTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCggg                   <  1:13720/140‑1 (MQ=255)

                                                                                                            aGCGTTGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGttt              >  1:267678/1‑140 (MQ=255)

                                                                                                                ttGGCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGCGTTTTATc          >  1:47030/1‑140 (MQ=255)

                                                                                                                   gCGTGTGTCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATcgcg       <  2:356168/140‑1 (MQ=255)

                                                                                                                     gtgtgtCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGt     <  1:401461/140‑1 (MQ=255)

                                                                                                                        tgtCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCAt  >  2:48050/1‑140 (MQ=255)

                                                                                                                        tgtCCAGGCAGCAGGGTGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCAt  >  1:759796/1‑140 (MQ=255)

TTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCAT  >  NC_000913/4513150‑4513409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A