Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F28 I1 R1
|
134 |
60.5 |
2175152 |
94.0% |
2044642 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,215 |
(G)8→9 |
noncoding (72/87 nt) |
leuP ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,207 | 1 | . | G | 95.6%
| 139.7
/ 0.3
| 45 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (23/20); total (24/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 |
AGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCC > NC_000913/4606067‑4606340
|
aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa < 2:392753/140‑1 (MQ=255)
gTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑g < 1:642189/140‑1 (MQ=255)
tAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggg‑gtgg > 1:80195/1‑140 (MQ=255)
tGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGa > 1:722360/1‑140 (MQ=255)
gcgagggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGaa < 2:412426/136‑1 (MQ=255)
cgagggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAAc > 1:250240/4‑140 (MQ=255)
agggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAAccc > 2:817142/2‑140 (MQ=255)
ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAAccccc > 1:674909/1‑140 (MQ=255)
ttGAACCCCCGCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGt < 1:685497/140‑1 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGt < 2:674909/140‑1 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGt < 1:658663/140‑1 (MQ=255)
aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAg > 1:548621/1‑140 (MQ=255)
aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAg < 1:50811/140‑1 (MQ=255)
ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAacac > 2:489004/1‑140 (MQ=255)
cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACt < 1:782801/140‑1 (MQ=255)
cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACt > 1:187986/1‑140 (MQ=255)
cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:250240/140‑1 (MQ=255)
cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 2:722360/140‑1 (MQ=255)
tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca > 2:991043/1‑140 (MQ=255)
tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGATTTGAACCCCCACGCCCGAAATAACACTAACACCtt > 2:1076506/1‑139 (MQ=37)
cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc < 2:187986/140‑1 (MQ=255)
cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt > 2:435226/1‑140 (MQ=255)
cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa > 2:264734/1‑140 (MQ=255)
gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc > 1:247930/1‑140 (MQ=255)
aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa < 1:435226/140‑1 (MQ=255)
cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAtt < 1:264734/140‑1 (MQ=255)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCCTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAACTGAAGCTAGCGCACGTAGCAATTcc > 2:351545/1‑140 (MQ=25)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTATTATTACTTTAAATGATATCCTCCCGTTTAGGGCGAG‑GGGG‑GGGGACTTCAACTCCCACGCACTAAATAACACTCGCACCTGGAGCAATTGCTGCTACCATTTcc > 2:131753/1‑140 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc > 1:607668/1‑139 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg > 1:56793/1‑140 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:991043/140‑1 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:489004/140‑1 (MQ=255)
tCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 1:601119/1‑140 (MQ=255)
cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 2:548621/140‑1 (MQ=255)
aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:247930/140‑1 (MQ=255)
aaTACCGCCGCATTCGAACAGTCATCTTACTTTAGTTGATATCGAAACGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:436917/140‑1 (MQ=17)
tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg > 2:208667/1‑140 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:755479/136‑1 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 2:755479/1‑136 (MQ=255)
cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGCCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg > 1:950965/1‑137 (MQ=255)
cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGCGGGGACTTGAACCCCCACGCCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg < 2:950965/137‑1 (MQ=255)
cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc < 1:817142/140‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACga > 2:661220/1‑139 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc < 1:1067297/135‑1 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc > 2:1067297/1‑135 (MQ=255)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGTGAACAATATAAACGAGGt > 2:702046/1‑140 (MQ=18)
cTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg > 2:618773/1‑140 (MQ=255)
tttGATATCGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg < 1:618773/140‑1 (MQ=255)
cGCCTCGTTTGGTGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCCATATCAACGTGGTTTTTGGTGCGA‑ggggggg > 2:203070/1‑140 (MQ=255)
gTGCGA‑‑GGGGTGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAAcc > 1:367438/1‑140 (MQ=255)
tGCGAG‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGAAccc > 2:308510/1‑140 (MQ=255)
|
AGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCC > NC_000913/4606067‑4606340
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
