Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F28 I1 R1
|
134 |
60.5 |
2175152 |
94.0% |
2044642 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,330 |
(G)8→9 |
noncoding (72/87 nt) |
leuQ ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,322 | 1 | . | G | 86.1%
| 124.4
/ 14.6
| 43 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (2/4); new base G (17/20); total (19/24) |
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCCTCGTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAG > NC_000913/4606191‑4606449
|
cGCCTCGTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCCATATCAACGTGGTTTTTGGTGCGA‑gg‑ggggg > 2:203070/1‑140 (MQ=255)
gTGCGA‑GGGGTGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAAcc > 1:367438/1‑140 (MQ=255)
tGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAAccc > 2:308510/1‑140 (MQ=255)
ggggggg‑gggaACTTGAACCCCCGCGTCTGTAAGAACACTAACACCTAAAGCTAGCACGTATACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCa < 2:867523/137‑1 (MQ=21)
gaggggg‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCa < 1:351545/137‑1 (MQ=255)
gaggggg‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCa < 1:308510/137‑1 (MQ=255)
aggggg‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCAc > 1:210039/3‑140 (MQ=255)
gggACTTGAACCCCCTCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGTGGGGGGACTTGAACCCCCACGTCCGTa < 2:56793/140‑1 (MQ=255)
gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTa < 2:244738/140‑1 (MQ=255)
gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTa < 2:367438/140‑1 (MQ=255)
gACTTGAACCCCCTCGTCCGTAAGAACACTAAAGCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAg < 2:607668/140‑1 (MQ=25)
cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGa > 1:681827/1‑140 (MQ=255)
aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt < 2:210039/140‑1 (MQ=255)
cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCTCCACCTTCGCCCAGTACAAGCAATATCAACGAGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa < 2:1081221/140‑1 (MQ=25)
acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg < 2:681827/140‑1 (MQ=255)
acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg < 1:203070/140‑1 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 2:807750/1‑134 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 1:807750/134‑1 (MQ=255)
acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGAGCGAGGG‑GGGGGGTCTTGAACCCCCACGTCCGTAAGGTCACTAACACCTGAAGCAAGcgcgcttatc > 2:122231/1‑134 (MQ=37)
tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 1:525394/1‑140 (MQ=255)
aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg > 2:980462/1‑126 (MQ=255)
aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg < 1:980462/126‑1 (MQ=255)
gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 2:574656/1‑140 (MQ=255)
gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 1:208667/140‑1 (MQ=255)
gtAGCGCGTCTACCAATTCCGCCCGCTTCGCCCAGTTCGTGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 2:601119/139‑1 (MQ=21)
tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa > 2:1082022/1‑140 (MQ=255)
tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa > 1:686238/1‑140 (MQ=255)
tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa > 1:408432/1‑140 (MQ=255)
ttCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 1:574656/140‑1 (MQ=255)
ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt < 1:702046/140‑1 (MQ=255)
cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 2:991938/122‑1 (MQ=255)
cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc > 1:991938/1‑122 (MQ=255)
ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt > 1:948842/1‑140 (MQ=255)
gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa < 2:525394/140‑1 (MQ=255)
ccAGTGCGATCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCGCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt < 1:1033652/140‑1 (MQ=255)
gCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa > 1:134964/1‑140 (MQ=255)
atCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg > 2:445985/1‑140 (MQ=255)
atCAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg > 1:191320/1‑140 (MQ=255)
cAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg > 2:160054/1‑140 (MQ=255)
cAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 1:218487/140‑1 (MQ=255)
cAACGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 1:160054/140‑1 (MQ=255)
aaCGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc < 2:686238/140‑1 (MQ=255)
aaCGTGGTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCAGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc < 2:408432/140‑1 (MQ=255)
gTTTTTGGTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTTAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa > 1:504424/1‑140 (MQ=255)
ggTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCGACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg < 2:346580/135‑1 (MQ=255)
ggTGCGAGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCGACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg > 1:346580/1‑135 (MQ=255)
|
CGCCTCGTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAG > NC_000913/4606191‑4606449
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
