Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	120	56.0	1898522	99.4%	1887130	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336,134	T→C	T300A (ACG→GCG)	adiC ←	arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,134	0	T	C	100.0%	76.0 / NA	22	T300A (ACG→GCG)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/1); total (21/1)

GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAACGCG  >  NC_000913/4336012‑4336266

gctgAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACg                                                                                                                     >  1:15260/1‑140 (MQ=255)

         gAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAgccgc                                                                                                            >  2:569320/1‑140 (MQ=255)

         gAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAgccgc                                                                                                            >  1:459218/1‑140 (MQ=255)

                             cgacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAAccc                                                                                        >  1:404100/1‑140 (MQ=255)

                              gacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCg                                                                                       >  1:745329/1‑140 (MQ=255)

                               acgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGc                                                                                      >  2:384772/1‑140 (MQ=255)

                                          ccccGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGa                                                                           >  2:39891/1‑140 (MQ=255)

                                               ccACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGaa                                                                      >  2:68925/1‑140 (MQ=255)

                                               ccACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGaa                                                                      >  1:744453/1‑140 (MQ=255)

                                                       gtccccGTTTTATTTCCACGGGCAAAAATCGGTGGGAACAGCCCGTCATCGGCAGCGGTTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGc                                                              <  2:744453/137‑1 (MQ=255)

                                                          cccGCTTTTTTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGcccc                                                           >  1:651482/1‑140 (MQ=255)

                                                            cGCTTTATTTACAGGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcgg                                                         <  2:606361/140‑1 (MQ=255)

                                                               tttatttaCACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcggcgg                                                      >  2:305862/1‑140 (MQ=255)

                                                               tttatttaCACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcggcgg                                                      >  1:457545/1‑140 (MQ=255)

                                                                  atttaCACGGGAAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGgtgt                                                   <  2:195313/140‑1 (MQ=255)

                                                                      acacGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCAcc                                               <  1:305862/140‑1 (MQ=255)

                                                                         cGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCaa                                            >  2:643341/1‑140 (MQ=255)

                                                                           ggCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACg                                          >  2:159246/1‑140 (MQ=255)

                                                                            gCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGc                                         >  2:236945/1‑140 (MQ=255)

                                                                                      gTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCg                               >  1:191178/1‑140 (MQ=255)

                                                                                       ggggAACAGCCCGCCACGGGCAGCGGCTTCCGCCGCTTGCCCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCgg                              <  2:457545/139‑1 (MQ=255)

                                                                                               gTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCg                      >  2:75682/1‑140 (MQ=255)

                                                                                                 ccGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGaa                    <  2:513982/140‑1 (MQ=255)

                                                                                                    ccagcggcggcggCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATgg                 <  2:745329/136‑1 (MQ=255)

                                                                                                        cGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGaa             >  2:9235/1‑140 (MQ=255)

                                                                                                          gCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGc           >  2:213809/1‑140 (MQ=255)

                                                                                                          gCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGc           >  1:150416/1‑140 (MQ=255)

                                                                                                              cGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGaa       >  2:236192/1‑140 (MQ=255)

                                                                                                                   ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  2:150416/140‑1 (MQ=255)

                                                                                                                   ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  1:776571/140‑1 (MQ=255)

                                                                                                                   ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  1:75682/140‑1 (MQ=255)

GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAACGCG  >  NC_000913/4336012‑4336266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A