Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 138 62.6 2119710 99.4% 2106991 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,596,427 A→G E146E (GAA→GAG)  lgoD → L‑galactonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,596,4270AG100.0% 169.4 / NA 46E146E (GAA→GAG) lgoDL‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/18);  total (28/18)

TGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAGGTGGTGGTG  >  NC_000913/4596302‑4596562
                                                                                                                             |                                                                                                                                       
tGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAg                                                                                                                           >  1:827454/1‑140 (MQ=255)
tGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAg                                                                                                                           >  1:827415/1‑140 (MQ=255)
  cTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAgcg                                                                                                                         <  2:313857/140‑1 (MQ=255)
  cTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGGGAGTATTTGAGCGTGCCGGGGGGGAACATTTTTCCCCCAGACGGGATTGACCCCCAGGCGGCAGCCTTAATTGAACCTTTCGCTATTAacg                                                                                                                         >  2:268818/1‑140 (MQ=255)
                    cATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTcgcg                                                                                                       <  2:696629/140‑1 (MQ=255)
                     aTTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCgcgc                                                                                                      <  1:268818/140‑1 (MQ=255)
                                       ggCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGa                                                                                    >  1:452180/1‑140 (MQ=255)
                                        gCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAg                                                                                   <  2:597860/140‑1 (MQ=255)
                                            ttttAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAgg                                                                               <  2:189864/140‑1 (MQ=255)
                                               tAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGc                                                                            <  2:918740/140‑1 (MQ=255)
                                                aGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCt                                                                           <  1:706078/140‑1 (MQ=255)
                                                 gTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCtg                                                                          <  1:181352/140‑1 (MQ=255)
                                                      tATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTggtggt                                                                     >  1:567314/1‑140 (MQ=255)
                                                      tATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTggtggt                                                                     >  1:860084/1‑140 (MQ=255)
                                                         ttGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCa                                                                                 >  1:649873/1‑125 (MQ=255)
                                                         ttGAGCGTGCCGGTGCCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCa                                                                                 <  2:649873/125‑1 (MQ=255)
                                                           gAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcgggg                                                                <  1:291750/140‑1 (MQ=255)
                                                                tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggcgggg                                                           >  2:124090/1‑140 (MQ=255)
                                                                tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggcgggg                                                           >  1:648261/1‑140 (MQ=255)
                                                                tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcagggcgggg                                                           >  2:766406/1‑140 (MQ=255)
                                                                tGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGCGGTcggggcgggg                                                           >  2:573539/1‑140 (MQ=255)
                                                                  ccGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGcc                                                         >  1:930840/1‑140 (MQ=255)
                                                                   cGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCa                                                        >  1:933763/1‑140 (MQ=255)
                                                                      tGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggc                                                               >  1:382620/1‑130 (MQ=255)
                                                                      tGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTcggggc                                                               <  2:382620/130‑1 (MQ=255)
                                                                         cGAACATTTTGCCCGCAGACGGTATTGACCCGCATGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGt                                                  <  1:278380/140‑1 (MQ=255)
                                                                                tttGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGgcg                                           >  2:103155/1‑140 (MQ=255)
                                                                                        cAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCa                                   <  1:660175/140‑1 (MQ=255)
                                                                                                     cccGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGGCTGGGCGCGGCGGCAATCGCTAAAGCCg                      >  1:673434/1‑140 (MQ=255)
                                                                                                       cGCAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAAACGCTAAAGCCGAt                    >  1:833122/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCaa                                  <  2:276245/124‑1 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCaa                                  >  1:276245/1‑124 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:184749/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:375589/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:495081/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:698568/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:245417/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:601798/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  1:293300/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:652179/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATgg                  >  2:695897/1‑140 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATg                   <  2:9796/139‑1 (MQ=255)
                                                                                                         cAGGCGGCAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATg                   >  1:9796/1‑139 (MQ=255)
                                                                                                            gcggcAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCAGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGgcgc               <  1:766406/140‑1 (MQ=255)
                                                                                                             cggcAGCATTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGca              <  1:901842/140‑1 (MQ=255)
                                                                                                                 agcaTTGATTGAGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAgg          <  1:184749/140‑1 (MQ=255)
                                                                                                                         ttgaGCCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGGCTGGGCGCCGCGGCAAGCGCTAAAGCCCCCGGTGCACCGggggcggtg  >  2:899044/1‑140 (MQ=255)
                                                                                                                             |                                                                                                                                       
TGCTGTGAAAAAATTTCAGTCATTGGCGTGCATCAGGATGGCGGTTTTAGTGAGTATTTGAGCGTGCCGGTGGCGAACATTTTGCCCGCAGACGGTATTGACCCGCAGGCGGCAGCATTGATTGAACCTTTCGCTATTAGCGCTCATGCGGTGCGTCGCGCAGCCATTGCTCCCGGCGAGCAGGTGCTGGTGGTCGGGGCGGGGCCAATCGGTCTGGGCGCGGCGGCAATCGCTAAAGCCGATGGCGCACAGGTGGTGGTG  >  NC_000913/4596302‑4596562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A